Fingerprint construction and analysis of genetic diversity with SSR markers for 46 japonica rice varieties in Zhejiang Province

Fingerprint construction and analysis of genetic diversity with SSR markers for 46 japonica rice varieties in Zhejiang Province

（1 College of Life and Environment Sciences， Hangzhou Normal University， Hangzhou 310036， China; 2 Institute of Crop and Nuclear Technology Utilization， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China; 3 College of Chemistry and Life Science， Zhejiang Normal University， Jinhua 321004， China; 4 Jiaxing Acaclemy of Agricultural Sciences, Jiaxing 314016, China）

Online:2014-09-25 Published:2014-10-11

Abstract

Abstract: In order to provide references for rice breeding in Zhejiang Province， 12 pairs of rice SSR primers recommended by Ministry of Agriculture （NY/T 1433—2007） were adopted in the present research to construct DNA fingerprints for 46 japonica rice varieties bred in Zhejiang Province since 2008， as well as to analyze their genetic relationship. It was shown that 84 alleles were detected by 12 pairs of SSR markers， and the average frequency of polymorphism （FP） was 0192. The similarity coefficient of 46 cultivars was between 0735-1000 with an average of 0879， which indicated a close affinity among the tested cultivars and a narrow breeding background in Zhejiang Province.

Key words: conventional japonica rice, fingerprint, SSR marker, genetic diversity

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Fingerprint construction and analysis of genetic diversity with SSR markers for 46 japonica rice varieties in Zhejiang Province

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