Abstract: The paper discussed the feasibility of esterase isozyme，polyphenol oxidase isozyme and peroxidase isozyme utilized in Clivia miniata classification and breeding adopting the polyacrylamide discontinuous system vertical slab electrophoresis technique by using different organs of Clivia miniata as the test materials. The results showed that the peroxidase isozyme pattern of mature leaves of Clivia miniata plants was clear and stable and this method was suitable for the application in Clivia miniata. The results of peroxidase isozyme system cluster analysis among different F1 crossbreds of Clivia miniata showed that 70% F1 tend to paternal，and 30% F1 variation occurred in Oil Carpenter×Round combination；50% F1 tend to parent，and 10% F1 tend to paternal in Round×Oil Carpenter combination；70% F1 tend to parent，20% F1 tend to paternal，and 10% F1 variation occurred in Erhualian×Round combination；Accordingly， the scientific basis was provided for the classification and breeding of Clivia miniata.

Key words: Clivia miniata, isozyme, F1, cluster analysis