Previous Articles     Next Articles

Cloning of HvSAMS gene from Hosta ventricosa flowers and its expression analysis

  

  1. (College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China)
  • Online:2015-06-25 Published:2015-06-26

Abstract: The full\|length of cDNA of SAMS gene from Hosta ventricosa flowers was obtained by RACE and RT\|PCR, which was designated as HvSAMS. Its open reading frame has 1 191 bp and is not interrupted by any intron, encoding a polypeptide of 396 amino acids. Multiple alignment analysis based on the amino acids indicated that HvSAMS had the similarity of 95% with that from Elaeagnus umbellata, Dendrobium crumenatum, and Lycoris radiata. Phylogenetic analysis showed that HvSAMS had the closest relation with that from Gladiolus grandiflorus. The expression patterns of HvSAMS were investigated by means of semi\|quantitative RT\|PCR. HvSAMS was expressed highly in flowers and leaves, moderately in roots, but poorly in stems. HvSAMS transcript was significantly down\|regulated in senescing flowers, but significantly up\|regulated in wounded leaves. HvSAMS may not be involved with ethylene production because the changes in HvSAMS expression were not closely related to ethylene emission.

Key words: Hosta ventricosa, S\, adenosyl\, L\, methionine synthetase, cloning, expression analysis