Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage

doi:10.3969/j.issn.1004-1524.2018.04.02

›› 2018, Vol. 30 ›› Issue (4): 528-536.DOI: 10.3969/j.issn.1004-1524.2018.04.02

• Plant Protection • Previous Articles Next Articles

Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage

WU Degong, FANG Wenhao, DU Junli^*, YU Haibing, WANG Changjin, LI Jinpeng, CHU Lina, TANG Li

College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China

Received:2017-08-01 Online:2018-04-20 Published:2018-04-19

Abstract

Abstract: Two different aphid resistant maize (Zea mays L.) varieties, Zhengdan 958(resistant variety) and Liyu 16(susceptible variety),were used in this study, and the dynamic changes of malondialdehyde (MDA), soluble sugar, chlorophyll (Chl), soluble protein and five protective enzymes were measured after different density of aphids (0,10,20,40,80 heads per plant) suction stress in three leaf stages. The results showed that: in the different density of aphids sucking stress, the soluble sugar content of Zhengdan 958 and Liyu 16 increased with the density of aphids, and the growth rate of Zhengdan 958 was less than Liyu 16. Chlorophyll content of two varieties decreased with aphid density increased, and the decrease rate of Liyu 16 was higher than that of Zhengdan 958. The change of soluble protein content was almost the same as the content of malondialdehyde (MDA), showing an upward trend, but the percentage change of MDA, soluble protein content in Zhengdan 958 was lower than that of Liyu 16.Therefore, there was a negative correlation between chlorophyll content and aphid density, while soluble sugar content, soluble protein and malondialdehyde (MDA) content were positively correlated with aphid density. The chlorophyll content change rate could be used as an index of aphid resistance, but the soluble sugar content, soluble protein and malondialdehyde (MDA) content were negatively correlated with the aphid resistance of maize. The activities of superoxide dismutase(SOD), peroxidase(PPO) and catalase(CAT) in Zhengdan 958 and Liyu 16 increased first and then decreased with the increase of aphid density. But the activities of five protective enzymes were higher than the control group; while the activity of polyphenol oxidase(PPO) and phenylalanine ammonia lyase(PAL) showed upward trend with the increase of aphid density. According to the results, it can be seen that the enzyme activity was related to the density of aphid at maize seedling stage. PPO and PAL play a leading role in the protective enzymes of maize, and SOD, POD and CAT play a protective role in lower aphid density.

Key words: Rhopalosiphum maidis, Zea mays, MDA, soluble sugar, chlorophyll, soluble protein, protective enzyme

CLC Number:

S433.1

WU Degong, FANG Wenhao, DU Junli, YU Haibing, WANG Changjin, LI Jinpeng, CHU Lina, TANG Li. Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage[J]. , 2018, 30(4): 528-536.

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Effects of different aphid density stress on physiological substances in resistant and susceptible maize varieties at seedling stage

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