SNP screening and bioinformatics analysis of MSTN in Japanese White Rabbit

doi:10.3969/j.issn.1004-1524.2018.12.05

›› 2018, Vol. 30 ›› Issue (12): 2018-2023.DOI: 10.3969/j.issn.1004-1524.2018.12.05

• Animal Science • Previous Articles Next Articles

SNP screening and bioinformatics analysis of MSTN in Japanese White Rabbit

REN Weihe, REN Wenwen, MA Boyan, GAO Chenghong, LI Hongxu, LIU Lixia, CAO Xin, ZHANG Li^*

School of Life Sciences and Engineering, Northwestern University of Nationalities, Lanzhou 730124, China

Received:2018-03-09 Online:2018-12-25 Published:2018-12-28

Abstract

Abstract: To investigate the relationship within MSTN gene and growth and meat quality traits in Japanese White Rabbit, a mixed DNA pool was built, PCR amplification and direct sequencing techniques were used to screen single nucleotide polymorphism (SNP) of this gene, and the SNP loci with significant effect on the meat quality of Japanese White Rabbit were screened. Functional prediction and homology analysis of proteins encoded by MSTN gene of Japanese White Rabbit were carried out at molecular level by bioinformatics method. The results showed that, the sequence length of MSTN gene coding region of Japanese White Rabbit was 1 128 bp, encoding 375 amino acids, which was an unstable water-soluble protein. The protein molecular weight was 42.8 ku, theoretical equal electric point was 6.97, protein tertiary structure was mixed type, and the irregular curl occupied the highest proportion. One SNP site was found in the non coding region of the amplification area, which did not affect the change of encoded amino acids. Homology analysis showed that, the similarity between MSTN gene of Japanese White Rabbit and common rabbit was 100%, and MSTN gene of Japanese White Rabbit had a higher similarity to the home cat and the kinship was close.

Key words: Japanese White Rabbit, MSTN gene, single nucleotide polymorphism, bioinformatics

CLC Number:

S823.8

REN Weihe, REN Wenwen, MA Boyan, GAO Chenghong, LI Hongxu, LIU Lixia, CAO Xin, ZHANG Li. SNP screening and bioinformatics analysis of MSTN in Japanese White Rabbit[J]. , 2018, 30(12): 2018-2023.

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SNP screening and bioinformatics analysis of MSTN in Japanese White Rabbit

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