Correlation analysis of HSP70 gene polymorphism and semen quality in Xuefeng Black-bone chicken

doi:10.3969/j.issn.1004-1524.2020.08.07

›› 2020, Vol. 32 ›› Issue (8): 1378-1384.DOI: 10.3969/j.issn.1004-1524.2020.08.07

• Animal Science • Previous Articles Next Articles

Correlation analysis of HSP70 gene polymorphism and semen quality in Xuefeng Black-bone chicken

YU Zonggang^1,2, JIANG Jun², YAO Yaling³, GUO Ying³, LI Chuang¹, YAN Haifeng^1,2,*

1. Hunan Institute of Animal and Veterinary Science, Changsha 410131, China;
2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
3. Hunan Hongjiang Songyun Poultry Industry Co., Ltd., Hongjiang 418200, China

Received:2020-02-26 Online:2020-08-25 Published:2020-08-28

Abstract

Abstract: To study HSP70 gene polymorphism of Xuefeng black-bone chicken and its relationship with frozen semen quality of chicken. Molecular markers related to freezing resistance of chicken semen were screened out to provide technical support for freezing preservation and molecular breeding of chicken semen. One hundred mature white Xuefeng black bone roosters of the same batch were selected. The semen volume, semen density and frozen semen vitality of rooster were measured. Blood was collected from the pterygium vein. gDNA was extracted from the blood sample and HSP70 gene was amplified. Three mixed pools of low, medium and high semen quality were constructed and sequenced. After sequencing a single sample, the genotyping was completed by comparing the sequencing peak diagram. The number of individuals with different genotypes was counted and the correlation analysis of semen quality was further carried out. The results showed that there was a mutation G>A at 436 bp in HSP70 gene, and G was the dominant gene. The population was divided into AA, AG and GG genotypes with the frequencies of 22%, 44% and 34%, respectively. The genetic diversity analysis PIC of this locus was 0.371 4, which was a moderate polymorphic locus. Hardy-Weinberg equilibrium fitness test showed χ²=1.1480 (P>0.05), which showed that the population conformed to Hardy-Weinberg equilibrium law. Correlation analysis between gene polymorphism and semen quality showed that the sperm volume and semen density of GG genotype individuals were significantly higher than those of AG type and AA type individuals (P<0.01). The viability after freezing of GG genotype individuals was significantly higher than that of AG type individuals (P<0.05), and was significantly higher than that of AA type individuals (P<0.01). The semen collection volume and semen density of AG type individuals were significantly higher than those of AA type individuals (P<0.01), and the difference of frozen semen activity was not significant. To sum up, the mutant G>A site of HSP70 gene was significantly related to the frozen semen quality of chicken. The locus could be used as a candidate gene for individual semen antifreeze of Xuefeng black-bone chicken. The individual with genotype GG had the best semen anti-freezing, followed by AG type individual.

Key words: HSP70 gene, SNP, semen quality, frozen semen, Xuefeng black-bone chicken, sequencing

CLC Number:

S831

YU Zonggang, JIANG Jun, YAO Yaling, GUO Ying, LI Chuang, YAN Haifeng. Correlation analysis of HSP70 gene polymorphism and semen quality in Xuefeng Black-bone chicken[J]. , 2020, 32(8): 1378-1384.

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Correlation analysis of HSP70 gene polymorphism and semen quality in Xuefeng Black-bone chicken

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