Effects of NaCl and Na2SO4 stress on content and distribution of K + and Na + of Echinochloa frumentacea seedlings

doi:10.3969/j.issn.1004-1524.2021.03.03

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 396-403.DOI: 10.3969/j.issn.1004-1524.2021.03.03

• Crop Science • Previous Articles Next Articles

Effects of NaCl and Na₂SO₄ stress on content and distribution of K ⁺ and Na ⁺ of Echinochloa frumentacea seedlings

LU Anqiao^a, ZHANG Fengju^b, WANG Xueqin^b, XU Xing^a^,^b^,^*()

a. College of Agronomy, Ningxia University, Ningxia 750021, China
b. Institute of Environment Engineering, Ningxia University, Ningxia 750021, China

Received:2020-08-28 Online:2021-04-02 Published:2021-03-25
Contact: XU Xing

Abstract

Abstract:

In order to explore the characteristic of K ⁺ and Na ⁺ absorption and distribution at seedling stage of Echinochloa frumentacea under salt stress. The variety Haizi-1 was used as test material, eight different concentrations (0, 25, 50, 75, 100, 125, 150, 200 mmol·L ^-1) of NaCl and Na₂SO₄ were set to simulate conditions of salt stress in this study. The results showed that with the increase of NaCl and Na₂SO₄ concentration, Na ⁺ content was increased, K ⁺ content was decreased, K ⁺/Na ⁺ ratio were all decreased in leaf, stem sheath and root; Content and distribution of Na ⁺ was root>stem sheath>leaf, Na ⁺ content in leaf and stem sheath were significantly lower than that in root. K ⁺/Na ⁺ ratio in leaf and stem sheath was higher than that in root. Content and distribution of K ⁺ was stem sheath>root>leaf under low salt concentration, and under the medium and high salt concentration, content and distribution of K ⁺ was stem sheath>leaf>root. Transport selectivity coefficient from root to stem sheath of K ⁺ and Na ⁺ (ST1_{K, Na}) and transport selectivity coefficient from stem sheath to leaf of K ⁺ and Na ⁺ (ST2_{K, Na}) under salt stress were significantly higher than those of the control at salt concentration>25 mmol·L ^-1, and ST1_{K, Na} was higher than ST2_{K, Na}. When the salt concentration was≥125 mmol·L ^-1, K ⁺/Na ⁺ ratio became stable in above ground under Na₂SO₄ stress, K ⁺ content in stem sheath and root and ST1_K,Na were higher than those under the same concentration of NaCl stress, and Na ⁺ content in leaf and stem sheath were lower than that under the same concentration of NaCl stress. ST2_{K, Na} showed an upward trend under Na₂SO₄ stress, and increased firstly and then decreased under NaCl stress. In conclusion, it had been shown that under salt stress, root system of E. frumentacea had a strong restriction on sodium ion transport to the above-ground part and had higher selective absorption capacity for K ⁺, and it had more tolerance to Na₂SO₄ stress than NaCl stress under higher concentration of salt stress.

Key words: salt stress, Echinochloa frumentacea, seedlings stage, K ⁺, Na ⁺, transport selectivity coefficient

CLC Number:

S516

LU Anqiao, ZHANG Fengju, WANG Xueqin, XU Xing. Effects of NaCl and Na₂SO₄ stress on content and distribution of K ⁺ and Na ⁺ of Echinochloa frumentacea seedlings[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 396-403.

Figures/Tables 4

Fig.1 Effect of salt stress on K+ content in different organs of Echinochloa frumentacea seedlings Different letters above bars indicated significant differences at P<0.05 under the same salt stress among different salt concentrations. The same as below.

Fig.2 Effect of salt stress on Na+ content in different organs of Echinochloa frumentacea seedlings

Table 1 Effect of salt stress on K+/Na+ ratio in different organs of the Echinochloa frumentacea seedlings

处理浓度 Treatment concentration/ (mmol·L^-1)	NaCl			Na₂SO₄
处理浓度 Treatment concentration/ (mmol·L^-1)	叶片K⁺/Na⁺ K⁺/Na⁺ in leaf	茎鞘 K⁺/Na⁺ K⁺/ $N_{a}^{+}$ in stem and sheath	根系K⁺/Na⁺ K⁺/Na⁺ in root	叶片K⁺/Na⁺ K⁺/Na⁺ in leaf	茎鞘K⁺/Na⁺ K⁺/Na⁺ in stem and sheath	根系K⁺/Na⁺ K⁺/Na⁺ in root
0	3.54±0.062 a	5.03±0.110 a	3.13±0.022 a	3.54±0.062 a	5.03±0.110 a	3.13±0.022 a
25	0.96±0.043 b	1.12±0.054 b	0.69±0.001 b	0.99±0.049 b	0.98±0.006 b	0.58±0.003 b
50	0.79±0.005 c	0.86±0.004 c	0.51±0.003 c	0.66±0.003 c	0.64±0.002 c	0.38±0.001 c
75	0.62±0.004 d	0.55±0.004 d	0.31±0.003 d	0.45±0.006 d	0.44±0.002 d	0.26±0.004 d
100	0.55±0.003 e	0.43±0.003 e	0.23±0.002 e	0.36±0.019 e	0.35±0.007 e	0.20±0.005 e
125	0.40±0.004 f	0.32±0.004 f	0.19±0.0001 f	0.36±0.003 e	0.34±0.001 e	0.17±0.001 f
150	0.33±0.009 g	0.27±0.006 f	0.16±0.006 g	0.37±0.004 e	0.35±0.004 e	0.16±0.001 fg
200	—	—	—	0.35±0.081 e	0.26±0.007 f	0.15±0.002 g

Table 1 Effect of salt stress on K+/Na+ ratio in different organs of the Echinochloa frumentacea seedlings

处理浓度 Treatment concentration/ (mmol·L^-1)	NaCl			Na₂SO₄
处理浓度 Treatment concentration/ (mmol·L^-1)	叶片K⁺/Na⁺ K⁺/Na⁺ in leaf	茎鞘 K⁺/Na⁺ K⁺/ $N_{a}^{+}$ in stem and sheath	根系K⁺/Na⁺ K⁺/Na⁺ in root	叶片K⁺/Na⁺ K⁺/Na⁺ in leaf	茎鞘K⁺/Na⁺ K⁺/Na⁺ in stem and sheath	根系K⁺/Na⁺ K⁺/Na⁺ in root
0	3.54±0.062 a	5.03±0.110 a	3.13±0.022 a	3.54±0.062 a	5.03±0.110 a	3.13±0.022 a
25	0.96±0.043 b	1.12±0.054 b	0.69±0.001 b	0.99±0.049 b	0.98±0.006 b	0.58±0.003 b
50	0.79±0.005 c	0.86±0.004 c	0.51±0.003 c	0.66±0.003 c	0.64±0.002 c	0.38±0.001 c
75	0.62±0.004 d	0.55±0.004 d	0.31±0.003 d	0.45±0.006 d	0.44±0.002 d	0.26±0.004 d
100	0.55±0.003 e	0.43±0.003 e	0.23±0.002 e	0.36±0.019 e	0.35±0.007 e	0.20±0.005 e
125	0.40±0.004 f	0.32±0.004 f	0.19±0.0001 f	0.36±0.003 e	0.34±0.001 e	0.17±0.001 f
150	0.33±0.009 g	0.27±0.006 f	0.16±0.006 g	0.37±0.004 e	0.35±0.004 e	0.16±0.001 fg
200	—	—	—	0.35±0.081 e	0.26±0.007 f	0.15±0.002 g

Fig.3 Effect of salt stress on K+, Na+ transport selectivity coefficient of the Echinochloa frumentacea seedling

References 29

[1]	MUNNS R . Genes and salt tolerance: bringing them together[J]. New Phytologist, 2005,167(3):645-663.
[2]	杨劲松 . 中国盐渍土研究的发展历程与展望[J]. 土壤学报, 2008,45(5):837-845.
	YANG J S . Development and prospect of the research on salt-affected soils in China[J]. Acta Pedologica Sinica, 2008,45(5):837-845.(in Chinese with English abstract)
[3]	魏国强, 朱祝军, 方学智 , 等. NaCl胁迫对不同品种黄瓜幼苗生长、叶绿素荧光特性和活性氧代谢的影响[J]. 中国农业科学, 2004,37(11):1754-1759.
	WEI G Q, ZHU Z J, FANG X Z , et al. The effects of NaCl stress on plant growth, chlorophyll fluorescence characteristics and active oxygen metabolism in seedlings of two cucumber cultivars[J]. Scientia Agricultura Sinica, 2004,37(11):1754-1759. (in Chinese with English abstract)
[4]	王佺珍, 刘倩, 高娅妮 , 等. 植物对盐碱胁迫的响应机制研究进展[J]. 生态学报, 2017,37(16):5565-5577.
	WANG Q Z, LIU Q, GAO Y N , et al. Review on the mechanisms of the response to salinity-alkalinity stress in plants[J]. Acta Ecologica Sinica, 2017,37(16):5565-5577.(in Chinese with English abstract)
[5]	李品芳, 侯振安, 龚元石 . NaCl胁迫对苜蓿和羊草苗期生长及养分吸收的影响[J]. 植物营养与肥料学报, 2001,7(2):211-217.
	LI P F, HOU Z A, GONG Y S . Effect of NaCl stress on growth and nutrient absorption in seedling of Medicago sativa and Leymus chinesis[J]. Plant Nutrition and Fertilizer Science, 2001,7(2):211-217.(in Chinese with English abstract)
[6]	王玉凤, 薛盈文, 杨克军 , 等. NaCl胁迫对玉米幼苗不同器官离子含量的影响[J]. 生态学杂志, 2011,30(8):1654-1661.
	WANG Y F, XUE Y W, YANG K J , et al. Effects of NaCl stress on ion contents in different organs of maize (Zea mays L.) seedlings[J]. Chinese Journal of Ecology, 2011,30(8):1654-1661. (in Chinese with English abstract)
[7]	张永亮, 聂微微, 任秀珍 , 等. 复盐胁迫下二种虉草K +、Na +吸收与运输的特点 [J]. 中国草地学报, 2010,32(3):28-33.
	ZHANG Y L, NIE W W, REN X Z , et al. Effects of mixed saline stress on the absorption and transportation of K + and Na + of two reed canarygrass [J]. Chinese Journal of Grassland, 2010,32(3):28-33. (in Chinese with English abstract)
[8]	MUNNS R, TERMAAT A . Whole-plant responses to salinity[J]. Functional Plant Biology, 1986,13(1):143.
[9]	陈少良, 李金克, 尹伟伦 , 等. 盐胁迫条件下杨树组织及细胞中钾、钙、镁的变化[J]. 北京林业大学学报, 2002,24(5):84-88.
	CHEN S L, LI J K, YIN W L , et al. Tissue and cellular K +, Ca ²+ and Mg ²+ of poplar under saline salt stress conditions [J]. Journal of Beijing Forestry University, 2002,24(5):84-88. (in Chinese with English abstract)
[10]	ZHU J K . Regulation of ion homeostasis under salt stress[J]. Current Opinion in Plant Biology, 2003,6(5):441-445. DOI URL PMID
[11]	陈惠哲 , NATALIA L, 朱德峰, 等. 盐胁迫下水稻苗期Na +和K +吸收与分配规律的初步研究 [J]. 植物生态学报, 2007,31(5):937-945.
	CHEN H Z, NATALIA L, ZHU D F , et al. Absorption and distribution of Na + and K + in rice seedling under salt stress [J]. Journal of Plant Ecology (Chinese Version), 2007,31(5):937-945. (in Chinese with English abstract)
[12]	万力生, 耿本仁, 邵生荣 , 等. 一种很有前途的草料兼用作物: 湖南稷子[J]. 宁夏农林科技, 1984(1):37-38.
	WAN L S, GENG B R, SHAO S R , et al. A promising dual-purpose forage and feed of Echinochloa frumentacea[J]. Ningxia Journal of Agriculture and Forestry Science and Technology, 1984(1):37-38.(in Chinese)
[13]	海宝, 双全 . 湖南稷子的栽培及利用[J]. 内蒙古草业, 1997(4):48-49.
	HAI B, SHUANG Q . Cultivation and utilization of Echinochloa frumentacea[J]. Grassland and Prataculture, 1997(4):48-49.(in Chinese)
[14]	万力生, 姜文奎, 刘升林 , 等. 海子1号湖南稷子高产性能的研究[J]. 草业科学, 1991(1):13-18.
	WAN L S, JIANG W K, LIU S L , et al. A study on the high-yield property of billon dollar grass[J]. Pratacultural Science, 1991(1):13-18. (in Chinese with English abstract)
[15]	张金林, 陈托兄, 严学兵 , 等 . 烯效唑( S3307)对湖南稷子整株水平Na +、K +选择性和游离脯氨酸分配的影响 [J]. 草业学报, 2006,15(2):42-47.
	ZHANG J L, CHEN T X, YAN X B , et al. Effects of uniconazole (S3307) on selectivity for Na +, K + and distribution of free proline in Echinochloa frumentacea [J]. Acta Prataculturae Sinica, 2006,15(2):42-47. (in Chinese with English abstract)
[16]	王锁民, 陈托兄, 张金林 . 6-苄氨基嘌呤(BA)和脱落酸(ABA)对湖南稷子Na +、K +选择性和游离脯氨酸分配的调节 [J]. 西北植物学报, 2004,24(4):588-595.
	WANG S M, CHEN T X, ZHANG J L . Regulation of 6 benzylaminopurine (BA) and abscisic acid (ABA) on selectivity for Na + over K + and the distribution of free proline in Echinochloa frumentacea [J]. Acta Botanica Boreali-Occidentalia Sinica, 2004,24(4):588-595. (in Chinese with English abstract)
[17]	王锁民, 沈禹颖, 朱兴运 . 湖南稷子离子吸收与分配特性研究[J]. 草业学报, 1995,4(2):71-74.
	WANG S M, SHEN Y Y, ZHU X Y . Studies on the features of ion absorption and distribution in Echinochioa frumentacea[J]. Acta Prataculturae Sinica, 1995,4(2):71-74. (in Chinese with English abstract)
[18]	李品芳, 杨志成 . NaCl胁迫下高羊茅生长及K +、Na +吸收与运输的动态变化 [J]. 草业学报, 2005,14(4):58-64.
	LI P F, YANG Z C . Dynamic effect of NaCl on absorption and transportation of K + and Na + in Festuca arundinacea [J]. Acta Prataculturae Sinica, 2005,14(4):58-64. (in Chinese with English abstract)
[19]	王明泉 . 寒地玉米种质资源苗期耐盐性评价与耐盐机制研究[D]. 大庆: 黑龙江八一农垦大学, 2019.
	WANG M Q . Salt tolerance evaluation and its mechanism study for maize germplasm in cold region at seedling stage[D]. Daqing: Heilongjiang Bayi Agricultural University, 2019. (in Chinese with English abstract)
[20]	王锁民, 朱兴运 . 碱茅离子吸收与分配特性研究[J]. 草业学报, 1994,3(1):39-43.
	WANG S M, ZHU X Y . Studies on the characteristics of ion absorption and distribution in Puccinellia tenuiflora[J]. Acta Prataculturae Sinica, 1994,3(1):39-43. (in Chinese with English abstract)
[21]	高永生, 王锁民, 张承烈 . 植物盐适应性调节机制的研究进展[J]. 草业学报, 2003,12(2):1-6.
	GAO Y S, WANG S M, ZHANG C L . Plant adaptive and regulatory mechanism under salt stress[J]. Acta Prataculturae Sinica, 2003,12(2):1-6. (in Chinese with English abstract)
[22]	赵春梅, 崔继哲, 金荣荣 . 盐胁迫下植物体内保持高K +/Na +比率的机制 [J]. 东北农业大学学报, 2012,43(7):155-160.
	ZHAO C M, CUI J Z, JIN R R . Mechanism of maintaining high K +/Na + ratios under salt stress in plant [J]. Journal of Northeast Agricultural University, 2012,43(7):155-160. (in Chinese with English abstract)
[23]	唐晓倩, 李焕勇, 杨秀艳 , 等. 短期NaCl胁迫对西伯利亚白刺幼苗Na +、K +分配和平衡的影响 [J]. 林业科学研究, 2017,30(6):1022-1027.
	TANG X Q, LI H Y, YANG X Y , et al. Effects of short-time salt stress on distribution and balance of Na + and K + in Nitraria sibirica Pall. seedlings [J]. Forest Research, 2017,30(6):1022-1027.(in Chinese with English abstract)
[24]	董秋丽, 夏方山, 董宽虎 . 盐胁迫对芨芨草种子萌发苗Na +, K +含量与质膜H +-ATPase活性的影响 [J]. 草地学报, 2010,18(6):823-828.
	DONG Q L, XIA F S, DONG K H . Effects of NaCl and Na₂SO₄ stress on the content of Na +, K + and plasma membrane H +-ATPase activity of Achnatherum splendens seedling [J]. Acta Agrectir Sinica, 2010,18(6):823-828. (in Chinese with English abstract)
[25]	董放, 曹靖, 李先婷 , 等. 不同盐分类型胁迫对豌豆幼苗离子吸收、累积及运输的影响[J]. 草业学报, 2016,25(11):66-75.
	DONG F, CAO J, LI X T , et al. Effects of various types of salt stress on ion absorption, accumulation and transportation in pea (Pisum sativum)seedlings[J]. Acta Prataculturae Sinica, 2016,25(11):66-75.(in Chinese with English abstract)
[26]	高雪, 朱林, 张会丽 . 盐胁迫对甜高粱和青贮玉米不同器官K +、Na +含量的影响 [J]. 河南农业科学, 2017,46(12):29-35.
	GAO X, ZHU L, ZHANG H L . Effects of soil saline stress on K + and Na + content of different organs of sweet Sorghum and silage corn [J]. Journal of Henan Agricultural Sciences, 2017,46(12):29-35.(in Chinese with English abstract)
[27]	王志春, 杨福, 陈渊 , 等. 苏打盐碱胁迫下水稻体内的Na +、K +响应 [J]. 生态环境, 2008,17(3):1198-1203.
	WANG Z C, YANG F, CHEN Y , et al. Sodium and potassium responses to sodicity stress in rice[J]. Ecology and Environment, 2008,17(3):1198-1203. (in Chinese with English abstract)
[28]	ZHANG J L, SHI H Z . Physiological and molecular mechanisms of plant salt tolerance[J]. Photosynjournal Research, 2013,115(1):1-22.
[29]	翁森红, 蒋尤泉, 王承斌 . 牧草耐盐性鉴定指标和方法的初步研究[J]. 中国草地, 1992(1):30-34.
	WENG S H, JIANG Y Q, WANG C B . A primary study of the identification of salt tolerant indexes and methods of forage crops[J]. Grassland of China, 1992(1):30-34. (in Chinese with English abstract)

Effects of NaCl and Na₂SO₄ stress on content and distribution of K ⁺ and Na ⁺ of Echinochloa frumentacea seedlings

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 29

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SONG Xindan, CHEN Binbin, MA Zengling, XU Lili, LIN Lidong, WU Mingjiang. Effects of salinity level on photosynthetic characteristics of Sargassum fusiforme seedlings [J]. , 2020, 32(9): 1634-1644.
[2]	SHI Jing, LIU Dongyang, ZHANG Fenghua. Physiological response and salt tolerance mechanism of cotton seedlings to salt stress [J]. , 2020, 32(7): 1141-1148.
[3]	BIAN Jianwen, CUI Yan, YANG Songqi, LUO Guanghong, MENG Xiangang. Effects of Chlamydomonas debaryana Gor. and Anabaena azotica Ley. on wheat seedling growth under salt stress [J]. , 2020, 32(10): 1748-1756.
[4]	ZHU Xiaolin, WEI Xiaohong, WANG Baoqiang, WANG Xian, ZHANG Mingjun. Transcriptome analysis of tomato under salt stress induced by c-GMP [J]. , 2020, 32(10): 1788-1797.
[5]	WANG Zhe, CHAI Li’ang, FAN Huaifu, DU Changxia. Progress in proteomics analysis of plant response to salt stress [J]. , 2019, 31(6): 1021-1028.
[6]	LI Yang, LIU Kai, WEI Jipeng, ZHANG Lan, LI Xin, HAN Wenyan, LI Qingyun. Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress [J]. , 2018, 30(7): 1160-1167.
[7]	LIN Yicheng, FU Qinglin, GUO Bin, LIU Chen, DING Nengfei. Effects of salt stress on anthocyanin content and activities of antioxidant enzymes in leaves of Photinia frasery [J]. , 2018, 30(6): 970-977.
[8]	WU Jianhua, WU Zhongxia, WANG Yuanhua, FENG Yingna, YAN Zhiming, CAI Shanya, WANG Quanzhi, SUN Ying. Effect of salicylic acid and proline on gene expression profiles in response to salt stress in lettuce [J]. , 2017, 29(9): 1489-1497.
[9]	SHAO Jincai, LIU Yuxia, YANG Jiaxin, XU Xinglong, ZHANG Qixiang, LI Qingwei. Effects of salt stress on germination and seeding of Chimonanthus praecox seeds [J]. , 2017, 29(7): 1139-1143.
[10]	GAO Fan, XIA Hui, YUAN Xuezhen, HUANG Shouyi, LIU Ji, LIANG Dong. Effects of exogenous melatonin on phenolic substance content and antioxidant ability of kiwifruit seedlings under salt stress [J]. , 2017, 29(7): 1144-1150.
[11]	XIAO Wenfei, NI Shen, QIU Jieren, WANG Shuzhen, XIN Ya, RUAN Songlin. Cloning and analysis of OsCSP2 gene promoter in rice (Oryza sativa L.) [J]. , 2017, 29(6): 857-863.
[12]	ZHANG Ling, WANG Hua, ZHOU Jing, XU Qiang. Effects of NaCl stress on chlorophyll fluorescence characteristics and physiological characteristics in seedlings of two pepper cultivars [J]. , 2017, 29(4): 597-604.
[13]	YAN Daoliang， ZHENG Bingsong. Effects of soaking seeds in trehalose on physiological characteristics of wheat Yangmai19 under salt stress [J]. , 2016, 28(8): 1271-.
[14]	MEI Yingxue， WEI Wei， ZHANG Shiwan， ZHANG Yunlu， WANG Jinyuan， WANG Qian， SU Xin， MA Lianju*. Effect of PEG pretreatment on antioxidant enzymes activity under salt stress in root of rice seedling [J]. , 2016, 28(8): 1304-.
[15]	ZHANG Jinxia1，2， DONG Dekun2， HU XingWang1，2， CHEN Fen2，3， ZHU Danhua2， *（. Research on salt tolerance of three soybean varieties at both germination and seedling stage [J]. , 2016, 28(7): 1101-.