Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress

doi:10.3969/j.issn.1004-1524.2021.03.07

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 429-436.DOI: 10.3969/j.issn.1004-1524.2021.03.07

• Horticultrual Science • Previous Articles Next Articles

Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress

GOU Bingdiao, DUAN Panpan, YANG Nan, ZHAO Shufang, WANG Yongfu, ZHANG Gaoyuan, WEI Bingqiang^*()

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2020-08-25 Online:2021-03-25 Published:2021-03-25
Contact: WEI Bingqiang

Abstract

Abstract:

In order to screen the photosynthetic parameters for identifying and predicting the heterosis of pepper, using the parents and their F₁ hybrid of pepper as materials, effects of low temperature and low light on net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular carbon dioxide concentration (C_i), transpiration rate (T_r) and chlorophyll content of pepper seedlings were comprehensively analyzed by the method of subordinate function values, grey correlation and correlation analysis. The results showed that the order of photosynthetic parameters to predict the heterosis of pepper from high to low was C_i, G_s, chlorophyll content, T_r and P_n. In addition, superiority from mid-parent heterosis and super-parent heterosis were chosen as the most suitable standards for appraising heterosis of pepper under low temperature and low light stress. The order of low temperature and low light resistance of the three materials was F₁ hybrid>male parent>female parent. This study enriched the heterosis theory of pepper, and provided theoretical basis for selecting photosynthetic parameters to predict the heterosis of pepper.

Key words: pepper, low temperature and low light stress, photosynthetic parameters, heterosis

CLC Number:

S641.3

GOU Bingdiao, DUAN Panpan, YANG Nan, ZHAO Shufang, WANG Yongfu, ZHANG Gaoyuan, WEI Bingqiang. Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 429-436.

Figures/Tables 9

Table 1 Subordinative function value and photosynthetic parameter values of three pepper accessions under low temperature and low light stress

样品 Sample	隶属函数值(X₀) Subordinative function value	P_n(X₁)/(μmol· m^-2·s^-1)	G_s (X₂)/(μmol· m^-2·s^-1)	C_i (X₃)/(μmol· mol^-1)	T_r(X₄)/(mmol· m^-2·s^-1)	C (X₅)
MP	0.724	5.003	0.035	326.629	1.682	41.433
FP	0.706	2.765	0.016	340.754	0.500	39.467
F₁	1.049	6.295	0.051	369.194	2.699	46.567

Table 2 Standardized results of grey correlation analysis of photosynthetic parameter values of different pepper materials under low temperature and low light stress

样品 Sample	X₀'	X₁'	X₂'	X₃'	X₄'	X₅'
MP	0.724	0.916	0.663	-2.446	0.387	-0.589
FP	0.706	-93.064	-7.187	-1.145	-26.752	-7.832
F₁	1.049	1.594	4.325	3.378	7.541	8.174

Table 3 Correlative modulus, correlative degree and correlative order between low temperature and low light resistance of different pepper materials and photosynthetic parameter values

样品 Sample	ξ₁	ξ₂	ξ₃	ξ₄	ξ₅
MP	1.000	0.996	0.933	1.001	0.989
FP	0.334	0.854	0.97	0.63	0.853
F₁	0.989	0.935	0.974	0.879	0.856
关联度	0.774	0.928	0.959	0.837	0.899
Correlative degree
关联序	5	2	1	4	3
Correlative order

Fig.1 Effects of low temperature and low light stress on net photosynthetic rate and heterosis of pepper leaves at seeding stage CK, Control; T1, Low temperature and low light treatment. Data on the bars marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.

Fig.2 Effects of low temperature and low light stress on stomatal conductance and heterosis of pepper leaves at seeding stage

Fig.3 Effects of low temperature and low light stress on Ci and heterosis of pepper leaves at seeding stage

Fig.4 Effects of low temperature and low light stress on transpiration rate and heterosis of pepper leaves at seeding stage

Fig.5 Effects of low temperature and low light stress on chlorophyll content and heterosis of pepper leaves at seeding stage

Table 4 Correlation analysis between photosynthetic relative indexes and heterosis of pepper at seedling stage under low temperature and low light stress

指标 Index	P_n	G_s	C_i	T_r	C	超中优势 Mid-parent heterosis	超亲优势 Super-parent heterosis
G_s	0.889^**
C_i	0.489	0.626
T_r	0.891^**	0.987^**	0.587
C	0.757^*	0.867^**	0.676^*	0.913^**
超中优势 Mid-parent heterosis	-0.355	-0.252	-0.006	-0.133	0.213
超亲优势 Super-parent heterosis	0.034	0.18	-0.071	0.251	0.466	0.866
离中优势 Superiority from mid-parent	0.707	0.573	-0.604	0.552	0.022	-0.113	-0.065

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Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress

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