Degradation and dietary risk of chlorpyrifos after its application during different periods of cowpea planting

doi:10.3969/j.issn.1004-1524.2021.06.16

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (6): 1104-1109.DOI: 10.3969/j.issn.1004-1524.2021.06.16

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Degradation and dietary risk of chlorpyrifos after its application during different periods of cowpea planting

WANG Di¹^,²(), DI Shanshan², WANG Xinquan², ZHANG Changpeng², WANG Xiangyun²^,^*(), WANG Meng³^,^*(), ZHANG Chenghui⁴

1. College of Horticulture, Hainan University, Haikou 570228, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory for Pesticide Residue Detection of Ministry of Agriculture and Rural Affairs, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Plant Protection, Hainan University, Haikou 570228, China
4. College of Food Science and Engineering, Hainan University, Haikou 570228, China

Received:2021-01-08 Online:2021-06-25 Published:2021-06-25
Contact: WANG Xiangyun,WANG Meng

Abstract

Abstract:

In recent years, the banned pesticide chlorpyrifos was frequently detected in cowpeas, which has become one of the key problems of high pesticide residue over standard rate in cowpeas. The degradation and risk assessment of chlorpyrifos in the different growth stages (sowing, seedling and podding) of cowpea were investigated. In field experiments, chlorpyrifos was applied to the different growth stages of cowpea. Cowpea samples were collected at mature stage, which were extracted with acetonitrile and purified by C18. The residue concentrations of chlorpyrifos was determined by UPLC-MS/MS, and dietary risk assessment was calculated. The limit of quantitation (LOQ) value of chlorpyrifos in cowpea samples was 0.01 mg·kg^-1. The average recoveries of chlorpyrifos in cowpea samples at four spiked levels (0.01, 0.1, 1 and 5 mg·kg^-1) ranged from 76.3% to 88.3% with relative standard deviation (RSD) in the range of 2.7%-6.4%. When applying chlorpyrifos at sowing stage, seedling stage and podding stage (one application and two applications), the residue concentrations of chlorpyrifos in cowpea samples were below LOQ. When once application at high podding stage, the degradation curve was Ct=1 726.6e^-0.431^t (R²=0.981 5), fitting the first-order kinetic equation, and the half-life was 1.6 d. Ten days after applying, the concentrations of chlorpyrifos in cowpea samples were below 0.05 mg·kg^-1. The risks of chronic and acute dietary intake were lower than 100%. Therefore, the applying of chlopyrifos at sowing period and seedling period of cowpea will not lead to the residual concentrations exceeding the MRL, but the preharvest interval was at least 10 days after applying chlopyrifos at podding period, and the use of chlorpyrifos in the peak pod period should be prohibited. In conclusion, it is very necessary to prohibit the use of chlorpyrifos in cowpea production in China under the background that the precise application of chlorpyrifos in cowpea production has not been formulated.

Key words: cowpea, chlorpyrifos, pesticide residue, risk assessment

CLC Number:

S643.4

WANG Di, DI Shanshan, WANG Xinquan, ZHANG Changpeng, WANG Xiangyun, WANG Meng, ZHANG Chenghui. Degradation and dietary risk of chlorpyrifos after its application during different periods of cowpea planting[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1104-1109.

Figures/Tables 3

References 22

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添加水平 Spiking level/(mg·kg^-1)	平均回收率 Average recovery/%	相对标准偏差 RSD/%
0.01	81.8	5.0
0.1	76.3	6.4
1	88.3	5.2
5	84.6	2.7

添加水平 Spiking level/(mg·kg^-1)	平均回收率 Average recovery/%	相对标准偏差 RSD/%
0.01	81.8	5.0
0.1	76.3	6.4
1	88.3	5.2
5	84.6	2.7

距最后 1 次施药后时间 Days after last application/d	残留量 Residue concentrations/(mg·kg^-1)	慢性膳食摄入风险 %ADI/%	急性膳食摄入风险 %ARfD/%
0	2.09	60.84	14.14
1	1.16	33.87	7.87
2	0.84	24.60	5.72
3	0.40	11.53	2.68
5	0.23	6.59	1.53
7	0.05	1.40	0.32
10	0.02	0.60	0.14
14	<0.01	0.17	0.04

距最后 1 次施药后时间 Days after last application/d	残留量 Residue concentrations/(mg·kg^-1)	慢性膳食摄入风险 %ADI/%	急性膳食摄入风险 %ARfD/%
0	2.09	60.84	14.14
1	1.16	33.87	7.87
2	0.84	24.60	5.72
3	0.40	11.53	2.68
5	0.23	6.59	1.53
7	0.05	1.40	0.32
10	0.02	0.60	0.14
14	<0.01	0.17	0.04

Degradation and dietary risk of chlorpyrifos after its application during different periods of cowpea planting

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