Establishment of a high temperature resistant family and determination of antioxidant capacity of razor clam Sinonovacula constricta

doi:10.3969/j.issn.1004-1524.2022.02.07

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (2): 266-274.DOI: 10.3969/j.issn.1004-1524.2022.02.07

• Animal Science • Previous Articles Next Articles

Establishment of a high temperature resistant family and determination of antioxidant capacity of razor clam Sinonovacula constricta

SHEN Benlong(), XUE Baobao, MENG Delong, SHEN Heding^*()

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, National Demonstration Center for Experimental Fisheries Science Education,Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, Shanghai 201306, China

Received:2020-09-04 Online:2022-02-25 Published:2022-03-02
Contact: SHEN Heding

Abstract

Abstract:

In order to continue to select the new high-temperature tolerant varieties s of razor clam Sinonovacula constricta Shenzhe No. 1, 16 full-sib families were established in the early stage of this experiment, and the growth of each family was recorded on the 2, 8, 12, 24, and 36 days. In the spat stage, 3 families of longer shell length and 3 families of shorter shell length were selected, and a total of 6 families were selected to the semi-lethal temperature experiment and determination of antioxidant capacity. The results showed that the survival rate of family 6 and 14 was significantly higher than that of other families at the spat stage, and the survival rate of family 14 was the highest. In the semi-lethal temperature test, the semi-lethal temperature of family 16 reached the highest of 36.64 ℃, and there was a significant difference in the semi-lethal temperature between families with long shell length and families with short shell length (P<0.05). Peroxidase (POD), total antioxidant capacity (T-AOC) and glutathione (GSH) increased with the increase of temperature. The three antioxidant indices of families with longer shell length were significantly higher than those of families with shorter shell length at high temperature (P<0.05). Through family selection and high temperature experiments, the offspring with resistance to high temperature were selected, and the determination of antioxidant capacity proved the necessity of family selection from the perspective of physiology and biochemistry. After comprehensively evaluating the shell length, survival rate and heat tolerance data of each family, it is finally determined that family 14 was the best family suitable for production, and family 15, family 16 could be used as candidate families.

Key words: Sinonovacula constricta, high temperature tolerance, family

CLC Number:

S968.31

SHEN Benlong, XUE Baobao, MENG Delong, SHEN Heding. Establishment of a high temperature resistant family and determination of antioxidant capacity of razor clam Sinonovacula constricta[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 266-274.

Figures/Tables 6

References 27

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家系编号 Family number	第2天 The 2nd day		第8天 The 8th day		第22天 The 22nd day		第36天 The 36th day
家系编号 Family number	SL/μm	SH/μm	SL/μm	SH/μm	SL/mm	SH/mm	SL/mm	SH/mm
1	135.58 ±8.58 a	105.51 ±5.93 ab	249.40 ±15.53 a	207.73 ±19.01 a	—	—	—	—
2	136.83 ±7.03 a	107.00 ±4.25 ab	252.36 ±20.15 a	209.20 ±19.53 ab	—	—	—	—
3	138.75 ±7.49 ab	109.00 ±5.38 b	261.36 ±12.3 bc	221.20 ±13.37 cde	2.15 ±0.41 ab	1.05 ±0.17 a	4.88 ±0.67 a	1.94 ±0.26 b
4	136.58 ±8.37 a	106.83 ±4.74 ab	261.43 ±9.75 bc	218.36 ±9.68 bcd	2.17 ±0.35 ab	1.06 ±0.16 a	4.97 ±0.54 a	1.99 ±0.22 ab
5	136.75 ±8.49 a	107.75 ±5.17 ab	256.36 ±12.93 ab	212.86 ±12.77 abc	2.07 ±0.22 a	1.03 ±0.11 a	4.76 ±1.16 a	1.90 ±0.46 a
6	138.00 ±8.32 a	107.08 ±4.56 ab	270.53 ±12.83 def	225.36 ±15.03 def	2.52 ±0.44 defg	1.23 ±0.20 bc	6.43 ±0.94 def	2.64 ±0.42 gi
7	138.33 ±7.61 ab	108.08 ±4.94 ab	264.20 ±15.60 bcde	219.86 ±15.78 cd	2.36 ±0.33 bcd	1.18 ±0.21 b	5.68 ±0.65 bc	2.28 ±0.26 cd
8	143.08 ±9.82 b	108.83 ±4.90 ab	271.36 ±12.75 def	225.90 ±12.08 def	2.57 ±0.62defg	1.28 ±0.28 bcd	6.54 ±1.13 defg	2.62 ±0.45 fgi
9	137.83 ±7.49a	107.58 ±4.85 ab	262.53 ±21.66 bcd	218.83 ±21.86 bcd	—	—	—	—
10	137.33 ±7.79 a	108.00 ±4.97 ab	270.53 ±15.49 ef	227.06 ±14.96 def	2.48 ±0.43 cdef	1.27 ±0.19 bcd	6.26 ±0.92 cde	2.51 ±0.36 efg
11	138.08 ±7.84 a	105.66 ±4.56 a	263.70 ±13.05 bcd	220.60 ±12.47 cd	2.24 ±0.26 abc	1.05 ±0.11 a	5.43 ±0.88 b	2.18 ±0.35 bc
12	136.33 ±7.92 a	106.50 ±4.50 ab	269.03 ±14.68 cdef	225.40 ±13.78 def	2.46 ±0.36 cdef	1.23 ±0.18 bc	5.96 ±0.93 bc	2.39 ±0.37 de
13	138.33 ±8.71 ab	106.50 ±4.72 ab	266.36 ±14.43 cdef	216.43 ±38.93 abcd	2.43 ±0.4 cdef	1.26 ±0.25 bcd	6.01 ±1.29 bcd	2.42 ±0.51 def
14	138.91 ±7.43 ab	108.33 ±5.37 ab	275.03 ±14.49 f	232.10 ±12.76 f	2.75 ±0.4 g	1.34 ±0.19 cd	7.04 ±0.72 g	2.83 ±0.29 i
15	139.33 ±7.90 ab	107.91 ±4.96 ab	274.70 ±14.35 f	232.06 ±13.38 f	2.71 ±0.61 fg	1.34 ±0.24 cd	6.93 ±1.31 fg	2.78 ±0.52 i
16	139.83 ±10.01 ab	107.16 ±4.68 ab	273.03 ±13.83 ef	231.03 ±13.31 ef	2.66 ±0.55 efg	1.36 ±0.25 d	6.71 ±0.85 efg	2.69 ±0.33 gi

家系编号 Family number	第2天 The 2nd day		第8天 The 8th day		第22天 The 22nd day		第36天 The 36th day
家系编号 Family number	SL/μm	SH/μm	SL/μm	SH/μm	SL/mm	SH/mm	SL/mm	SH/mm
1	135.58 ±8.58 a	105.51 ±5.93 ab	249.40 ±15.53 a	207.73 ±19.01 a	—	—	—	—
2	136.83 ±7.03 a	107.00 ±4.25 ab	252.36 ±20.15 a	209.20 ±19.53 ab	—	—	—	—
3	138.75 ±7.49 ab	109.00 ±5.38 b	261.36 ±12.3 bc	221.20 ±13.37 cde	2.15 ±0.41 ab	1.05 ±0.17 a	4.88 ±0.67 a	1.94 ±0.26 b
4	136.58 ±8.37 a	106.83 ±4.74 ab	261.43 ±9.75 bc	218.36 ±9.68 bcd	2.17 ±0.35 ab	1.06 ±0.16 a	4.97 ±0.54 a	1.99 ±0.22 ab
5	136.75 ±8.49 a	107.75 ±5.17 ab	256.36 ±12.93 ab	212.86 ±12.77 abc	2.07 ±0.22 a	1.03 ±0.11 a	4.76 ±1.16 a	1.90 ±0.46 a
6	138.00 ±8.32 a	107.08 ±4.56 ab	270.53 ±12.83 def	225.36 ±15.03 def	2.52 ±0.44 defg	1.23 ±0.20 bc	6.43 ±0.94 def	2.64 ±0.42 gi
7	138.33 ±7.61 ab	108.08 ±4.94 ab	264.20 ±15.60 bcde	219.86 ±15.78 cd	2.36 ±0.33 bcd	1.18 ±0.21 b	5.68 ±0.65 bc	2.28 ±0.26 cd
8	143.08 ±9.82 b	108.83 ±4.90 ab	271.36 ±12.75 def	225.90 ±12.08 def	2.57 ±0.62defg	1.28 ±0.28 bcd	6.54 ±1.13 defg	2.62 ±0.45 fgi
9	137.83 ±7.49a	107.58 ±4.85 ab	262.53 ±21.66 bcd	218.83 ±21.86 bcd	—	—	—	—
10	137.33 ±7.79 a	108.00 ±4.97 ab	270.53 ±15.49 ef	227.06 ±14.96 def	2.48 ±0.43 cdef	1.27 ±0.19 bcd	6.26 ±0.92 cde	2.51 ±0.36 efg
11	138.08 ±7.84 a	105.66 ±4.56 a	263.70 ±13.05 bcd	220.60 ±12.47 cd	2.24 ±0.26 abc	1.05 ±0.11 a	5.43 ±0.88 b	2.18 ±0.35 bc
12	136.33 ±7.92 a	106.50 ±4.50 ab	269.03 ±14.68 cdef	225.40 ±13.78 def	2.46 ±0.36 cdef	1.23 ±0.18 bc	5.96 ±0.93 bc	2.39 ±0.37 de
13	138.33 ±8.71 ab	106.50 ±4.72 ab	266.36 ±14.43 cdef	216.43 ±38.93 abcd	2.43 ±0.4 cdef	1.26 ±0.25 bcd	6.01 ±1.29 bcd	2.42 ±0.51 def
14	138.91 ±7.43 ab	108.33 ±5.37 ab	275.03 ±14.49 f	232.10 ±12.76 f	2.75 ±0.4 g	1.34 ±0.19 cd	7.04 ±0.72 g	2.83 ±0.29 i
15	139.33 ±7.90 ab	107.91 ±4.96 ab	274.70 ±14.35 f	232.06 ±13.38 f	2.71 ±0.61 fg	1.34 ±0.24 cd	6.93 ±1.31 fg	2.78 ±0.52 i
16	139.83 ±10.01 ab	107.16 ±4.68 ab	273.03 ±13.83 ef	231.03 ±13.31 ef	2.66 ±0.55 efg	1.36 ±0.25 d	6.71 ±0.85 efg	2.69 ±0.33 gi

Establishment of a high temperature resistant family and determination of antioxidant capacity of razor clam Sinonovacula constricta

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