Comparative studies of polysaccharides of three Dendrobium species from Zhejiang Province and exploration of their physiological functions

doi:10.3969/j.issn.1004-1524.20221009

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (8): 1888-1895.DOI: 10.3969/j.issn.1004-1524.20221009

• Food Science • Previous Articles Next Articles

Comparative studies of polysaccharides of three Dendrobium species from Zhejiang Province and exploration of their physiological functions

LI Jingrui¹(), TAO Wenyang², YANG Ying², ZHOU Wanyi², LU Shengmin²^,^*(), WANG Yangguang¹^,^*()

1. College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
2. Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-07-07 Online:2023-08-25 Published:2023-08-29

Abstract

Abstract:

To comparatively study the basic physicochemical properties and immunological efficacy of the three Dendrobium officinale(D. officinale) stems polysaccharides from the industrial base of Yandang Mountain, and to provide reference for their precise utilization, in this paper, polysaccharides from D. officinale cv. Yanhu 1 (DOP1), D. officinale cv. Yanhu 3 (DOP2) and D. officinale cv. Shenglan 8 (DOP3) were extracted. Their monosaccharide composition, total sugar content, molecular weight and monosaccharide linkages were respectively analyzed by high performance liquid chromatography (HPLC), phenol-sulfuric acid method, high performance gel permeation chromatography (HPGPC) and fourier transform infrared spectrometer (FT-IR). Their effects on immune function were further investigated. The results showed that all the three polysaccharides contained three polysaccharide fractions with different relative molecular weights, and DOP2 having the highest relative molecular weights. All the three DOP consisted of four monosaccharides, including arabinose, galactose, glucose and mannose, but they had different monosaccharide ratios. Their results of FT-IR spectra were almost the same, which showed the presence of pyran heteropolysaccharides with a predominantly β-configuration. All the three polysaccharides had certain proliferative effects on RAW 264.7 cells and the DOP3 was the best (P<0.000 1). In addition, DOP3 (1 600 μg·mL^-1) significantly promoted the release of NO from RAW 264.7 cells (P<0.05). The results indicated that there were significant differences in the physicochemical properties and immune efficacy of the three polysaccharides, and the immunomodulatory activity of DOP3 was superior to the other two Dendrobium polysaccharides, which might be related to their differences in structure and composition.

Key words: Dendrobium officinale, polysaccharide, molecular weight, monosaccharide composition, immunomodulation

CLC Number:

S567

LI Jingrui, TAO Wenyang, YANG Ying, ZHOU Wanyi, LU Shengmin, WANG Yangguang. Comparative studies of polysaccharides of three Dendrobium species from Zhejiang Province and exploration of their physiological functions[J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1888-1895.

Figures/Tables 6

Fig.1 The monosaccharide composition of three polysaccharides from Dendrobium stem including DOP1, DOP2 and DOP3

Table 1 Total sugar content in DOP

指标Index	DOP1	DOP2	DOP3
Glc含量 Glc content/%	36.23 ±0.69	50.43 ±0.35	32.59 ±0.58
Man含量 Man content/%	55.46 ±0.51	44.35 ±0.49	51.17 ±0.62
总糖含量 Total sugar content/%	91.69 ±1.20	94.78 ±0.84	83.76 ±1.20

Fig.2 High performance gel permeation chromatogram of three polysaccharides from Dendrobium stem including DOP1, DOP2 and DOP3

Fig.3 FT-IR spectra of three polysaccharides from Dendrobium stem including DOP1 (a), DOP2 (b) and DOP3 (c)

Fig.4 Effects of three polysaccharides from Dendrobium stem including DOP1 (a), DOP2 (b) and DOP3 (c) on proliferations rate in RAW 264.7 cell

Fig.5 Effect of three polysaccharides from Dendrobium stem including DOP1 (a), DOP2 (b) and DOP3 (c) on NO release from RAW 264.7 cells

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Comparative studies of polysaccharides of three Dendrobium species from Zhejiang Province and exploration of their physiological functions

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