Transcriptome sequencing and analysis of potential pathogenicity-related genes in Isaria fumosorosea

doi:10.3969/j.issn.1004-1524.20221707

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (9): 2169-2180.DOI: 10.3969/j.issn.1004-1524.20221707

• Plant Protection • Previous Articles Next Articles

Transcriptome sequencing and analysis of potential pathogenicity-related genes in Isaria fumosorosea

XIE Meiqiong¹(), WANG Longjiang², HE Yurong³, LYU Lihua⁴

1. College of Life Sciences and Resources and Environment, Yichun University, Yichun 336000, Jiangxi, China
2. College of Chemistry and Bioengineering, Yichun University, Yichun 336000, Jiangxi, China
3. College of Plant Protection, South China Agricultural University, Guangzhou 510642, China
4. Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China

Received:2022-11-29 Online:2023-09-25 Published:2023-10-09

Abstract

Abstract:

In order to explore the expression patterns of transcriptome genes of Isaria fumosorosea and to provide data for the functional study of I. fumosorosea, the transcriptom of I. fumosorosea IFCF01 was sequenced and analyzed using high-throughput non-parametric sequencing technology. A total of 540 896 444 clean reads were obtained by transcriptome sequencing. A total of 36 466 unigenes with the total length of 34 551 468 bp and an average length of 947 bp were obtained after assembly. The functional annotation showed that a total of 22 741 unigenes were annotated in 6 major databases,such as Nr, GO, COG and KEGG, accounting for 62.36% of the total unigenes. A total of 21 737 unigenes were annotated in the Nr database, with the highest number of anotation to Beauveria bassiana. 10 888 unigenes were annotated to 25 functional categories in COG database, and the number of genes involved in general function predicted only was the largest, matching on 4 060 unigenes. The GO annotation shoewed that a total of 9 617 unigenes were annotated, distributed in 49 subcategories of three functional modules: biological process function, molecular function and cell composition. A total of 14 364 unigenes were annotated in the KEGG database, involving 108 KEGG metabolic pathways. With the help of pathogen-host interaction gene database, a total of 86 unigenes associated with the potential pathogenicity of I. fumosorosea were identified. A total of 8 572 simple sequence repeats (SSR) loci were detected in transcriptome data of I. fumosorosea, with a frequency of 18.18%. The results of this study could lay the foundation for gene resources mining, functional gene research and SSR molecular markers for I. fumosorosea.

Key words: Isaria fumosorosea, transcriptome sequencing, gene, SSR molecular marker

CLC Number:

S476.12

XIE Meiqiong, WANG Longjiang, HE Yurong, LYU Lihua. Transcriptome sequencing and analysis of potential pathogenicity-related genes in Isaria fumosorosea[J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2169-2180.

Figures/Tables 10

References 34

[1]	郑宇, 丁雪玲, 姚凤銮, 等. 玫烟色棒束孢FZ-01鉴定及对烟粉虱的防治潜力评估[J]. 中国生物防治学报, 2019, 35(6): 876-883.
	ZHENG Y, DING X L, YAO F L, et al. Identification of Isaria fumosorosea FZ-01 and evaluation of its bio-control potential against Bemisia tabaci[J]. Chinese Journal of Biological Control, 2019, 35(6): 876-883. (in Chinese with English abstract)
[2]	王宏民, 赵义涛, 郭恒, 等. 两种表面活性剂对玫烟色棒束孢PF904菌株侵染小菜蛾幼虫的影响[J]. 昆虫学报, 2021, 64(2): 205-212.
	WANG H M, ZHAO Y T, GUO H, et al. Influence of two surfactants on the infection of Isaria fumosoroseus strain PF904 on Plutella xylostella (Lepidoptera: Plutellidae) larvae[J]. Acta Entomologica Sinica, 2021, 64(2): 205-212. (in Chinese with English abstract)
[3]	曹娜. 玫烟色棒束孢发酵条件及对刺吸式口器害虫控制潜力研究[D]. 合肥: 安徽农业大学, 2018.
	CAO N. Study on diphasic fermentation of Isaria fumosorosea and control potential to piercing-sucking mouthpart pests[D]. Hefei: Anhui Agricultural University, 2018. (in Chinese with English abstract)
[4]	王成. 玫烟色棒束孢的遗传多态性[D]. 贵阳: 贵州大学, 2017.
	WANG C. Genetic polymorphism of Isaria fumosorosea[D]. Guiyang: Guizhou University, 2017. (in Chinese with English abstract)
[5]	韩志慧. 玫烟色棒束孢IF-1106代谢蛋白粗提物的杀虫活性研究[D]. 太谷: 山西农业大学, 2019.
	HAN Z H. Study on insecticidal activity of crude extract of metabolic protein of Isaria fumosorosea IF-1106[D]. Taigu: Shanxi Agricultural University, 2019. (in Chinese with English abstract)
[6]	赵义涛, 王宏民, 郭恒, 等. 虫生真菌玫烟色棒束孢PF904表面活性剂的筛选[J]. 农药学学报, 2020, 22(1): 154-162.
	ZHAO Y T, WANG H M, GUO H, et al. Screening of surfactants for entomogenous fungus Isaria fumosorosea PF₉₀₄[J]. Chinese Journal of Pesticide Science, 2020, 22(1): 154-162. (in Chinese with English abstract)
[7]	么瑞娜. 玫烟色棒束孢cDNA文库的构建及与小菜蛾Serpin基因的互作因子筛选[D]. 广州: 华南农业大学, 2017.
	YAO R N. Construction of cDNA library of Isaria fumosorosea and screening of interaction factors from library against serpin fromplutella xylostella[D]. Guangzhou: South China Agricultural University, 2017. (in Chinese with English abstract)
[8]	黄宇. 玫烟色棒束孢Ifcdp1基因的功能研究[D]. 广州: 华南农业大学, 2017.
	HUANG Y. Functional alanlysis of Ifcdp1 gene from Isaria fumosorosea[D]. Guangzhou: South China Agricultural University, 2017. (in Chinese with English abstract)
[9]	孟祥云. 玫烟色棒束孢几丁质酶Ⅱ基因的克隆、表达及两种几丁质酶的诱导转录[D]. 合肥: 安徽农业大学, 2011.
	MENG X Y. Cloning and expression of the chitinase Ⅱ genes from Isaria fumosorosea and inductive transcription of two chitinase genes[D]. Hefei: Anhui Agricultural University, 2011. (in Chinese with English abstract)
[10]	汤强, 刘玉军, 方玲, 等. 玫烟色棒束孢几丁质酶基因上游调控序列克隆与结构预测分析[J]. 热带作物学报, 2012, 33(1): 99-106.
	TANG Q, LIU Y J, FANG L, et al. Cloning and sequence analysis of the chitinase gene upstream from Isaria fumosorosea[J]. Chinese Journal of Tropical Crops, 2012, 33(1): 99-106. (in Chinese with English abstract)
[11]	董章勇, 舒永馨, 陈欣瑜, 等. 玫烟色棒束孢基因组候选效应因子的预测分析[J]. 西北农业学报, 2019, 28(12): 2078-2083.
	DONG Z Y, SHU Y X, CHEN X Y, et al. Prediction and analysis of candidate effectors from genome of Isaria fumosorosea[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2019, 28(12): 2078-2083. (in Chinese with English abstract)
[12]	GAO Q, JIN K, YING S H, et al. Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M. acridum[J]. PLoS Genetics, 2011, 7(1): e1001264.
[13]	ZHENG P, XIA Y L, XIAO G H, et al. Genome sequence of the insect pathogenic fungus Cordyceps militaris, a valued traditional Chinese medicine[J]. Genome Biology, 2011, 12(11): R116.
[14]	XIAO G H, YING S H, ZHENG P, et al. Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana[J]. Scientific Reports, 2012, 2: 483.
[15]	HAO Z G, LI Y Y, JIANG Y Y, et al. Genome sequence analysis of the fungal pathogen Fusarium graminearum using Oxford nanopore technology[J]. Journal of Fungi, 2021, 7(9): 699.
[16]	SHANG Y F, XIAO G H, ZHENG P, et al. Divergent and convergent evolution of fungal pathogenicity[J]. Genome Biology and Evolution, 2016, 8(5): 1374-1387.
[17]	GRABHERR M G, HAAS B J, YASSOUR M, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology, 2011, 29(7): 644-652.
[18]	WINNENBURG R, URBAN M, BEACHAM A, et al. PHI-base update: additions to the pathogen-host interaction database[J]. Nucleic Acids Research, 2008, 36(suppl_1): D572-D576.
[19]	XIANG L X, HE D, DONG W R, et al. Deep sequencing-based transcriptome profiling analysis of bacteria-challenged Lateolabrax japonicus reveals insight into the immune-relevant genes in marine fish[J]. BMC Genomics, 2010, 11: 472.
[20]	ZIMMERMANN G. The entomopathogenic fungi Isaria farinosa(formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control[J]. Biocontrol Science and Technology, 2008, 18(9): 865-901.
[21]	陈纬, 袁文静, 关雪, 等. 土壤中棒束孢菌的生物多样性及对黄曲条跳甲的活性[J]. 华南农业大学学报, 2021, 42(4): 75-82.
	CHEN W, YUAN W J, GUAN X, et al. Biodiversity of Isaria in soil and its activity against Phyllotreta striolata[J]. Journal of South China Agricultural University, 2021, 42(4): 75-82. (in Chinese with English abstract)
[22]	王滨, 曹娜, GHULAM Ali Bugti. 玫烟色棒束孢对多种刺吸式口器害虫防治潜力的室内评测[J]. 中国生物防治学报, 2018, 34(1): 86-91.
	WANG B, CAO N, BUGTI G. Laboratory assessment on virulence of Isaria fumosorosea against multiple species of sucking insects[J]. Chinese Journal of Biological Control, 2018, 34(1): 86-91. (in Chinese with English abstract)
[23]	王宏民, 李赫, 张天浩, 等. 玫烟色棒束孢Pr1酶活力、Pr1酶基因表达量与其毒力的相关性[J]. 生物工程学报, 2019, 35(1): 114-120.
	WANG H M, LI H, ZHANG T H, et al. Correlation between Pr1 protease activity, Pr1 gene expression and strain virulence of Isaria fumosorosea[J]. Chinese Journal of Biotechnology, 2019, 35(1): 114-120. (in Chinese with English abstract)
[24]	汤强. 玫烟色棒束孢毒力基因的克隆与表达及球孢白僵菌高毒力重组菌株的获得[D]. 合肥: 安徽农业大学, 2009.
	TANG Q. Cloning and expression of the virulence genes from Isaria fumosorosea and generation of transgenic Beauveria bassiana strains with these genes[D]. Hefei: Anhui Agricultural University, 2009. (in Chinese with English abstract)
[25]	吕利华, 何余容, 武亚敬, 等. 玫烟色拟青霉对小菜蛾致病力的时间-剂量-死亡率模型模拟[J]. 昆虫学报, 2007, 50(6): 567-573.
	LV L H, HE Y R, WU Y J, et al. The time-dose-mortality model of a Paecilomyces fumosoroseus isolate on the diamondback moth, Plutella xylostella[J]. Acta Entomologica Sinica, 2007, 50(6): 567-573. (in Chinese with English abstract)
[26]	李晓艳. 越橘果实转录组文库Solexa测序及花色素苷合成相关基因的表达分析[D]. 长春: 吉林农业大学, 2012.
	LI X Y. De novo characterization of blueberry fruit transcriptome using solexa sequencing to discover putative genes related to anthocyanins[D]. Changchun: Jilin Agricultural University, 2012. (in Chinese with English abstract)
[27]	SHI C Y, YANG H, WEI C L, et al. Deep sequencing of the Camellia sinensis transcriptome revealed candidate genes for major metabolic pathways of tea-specific compounds[J]. BMC Genomics, 2011, 12: 131.
[28]	许端祥, 赵瑞丽, 陈中钐, 等. 瓠瓜幼叶转录组功能基因测序及分析[J]. 核农学报, 2020, 34(6): 1163-1177.
	XU D X, ZHAO R L, CHEN Z S, et al. Transcriptome sequencing and analysis of functional genes in young leaves of bottle gourd[J]. Journal of Nuclear Agricultural Sciences, 2020, 34(6): 1163-1177. (in Chinese with English abstract)
[29]	陈艺荃, 方能炎, 叶秀仙, 等. 基于转录组测序的文心兰花香形成分析[J]. 核农学报, 2022, 36(3): 578-588.
	CHEN Y Q, FANG N Y, YE X X, et al. Analysis of floral scent formation in Oncidium orchid based on transcriptome sequencing[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(3): 578-588. (in Chinese with English abstract)
[30]	周雁, 范秀芝, 陈连福, 等. SSR在黑木耳和毛木耳转录组中的分布和序列特征[J]. 菌物学报, 2014, 33(2): 280-288.
	ZHOU Y, FAN X Z, CHEN L F, et al. Distribution and sequence characteristics of SSR in the transcriptomes of Auricularia auricula-judae and Auricularia polytricha[J]. Mycosystema, 2014, 33(2): 280-288. (in Chinese with English abstract)
[31]	李强, 陈诚, 熊川, 等. 杏鲍菇转录组数据SSR位点的生物信息学分析[J]. 应用与环境生物学报, 2017, 23(3): 454-458.
	LI Q, CHEN C, XIONG C, et al. Bioinformatic analysis of simple sequence repeat(SSR) loci in the Pleurotus eryngii transcriptome[J]. Chinese Journal of Applied and Environmental Biology, 2017, 23(3): 454-458. (in Chinese with English abstract)
[32]	张涵, 王芳, 白静, 等. 冬虫夏草转录组SSR位点信息分析研究[J]. 中国中药杂志, 2019, 44(21): 4605-4611.
	ZHANG H, WANG F, BAI J, et al. SSR loci information analysis in transcriptome of Cordyceps sinensis[J]. China Journal of Chinese Materia Medica, 2019, 44(21): 4605-4611. (in Chinese with English abstract)
[33]	李青竹, 蔡友铭, 张永春, 等. 基于石蒜属转录组序列的SSR分子标记开发应用[J]. 核农学报, 2021, 35(9): 2002-2015.
	LI Q Z, CAI Y M, ZHANG Y C, et al. Development and application of SSR molecular markers based on transcriptome sequencing of Lycoris spp[J]. Journal of Nuclear Agricultural Sciences, 2021, 35(9): 2002-2015. (in Chinese with English abstract)
[34]	金群力, 沈颖越, 蔡为明, 等. 金针菇基因组SSR位点分析及标记开发[J]. 食用菌学报, 2016, 23(2): 12-19.
	JIN Q L, SHEN Y Y, CAI W M, et al. Distribution pattern analysis and marker development of SSR loci in the Flammulina velutipes genome[J]. Acta Edulis Fungi, 2016, 23(2): 12-19. (in Chinese with English abstract)

数据库Database	数量Number	比例Proportion/%
Nr	21 737	59.61
Nt	12 419	34.06
Swiss-Prot	14 243	39.06
KEGG	14 364	39.39
COG	10 888	29.86
GO	9 617	26.37
合计Total	22 741	62.36

数据库Database	数量Number	比例Proportion/%
Nr	21 737	59.61
Nt	12 419	34.06
Swiss-Prot	14 243	39.06
KEGG	14 364	39.39
COG	10 888	29.86
GO	9 617	26.37
合计Total	22 741	62.36

KEGG通路 KEGG pathway	数量 Number	比例 Proportion/%	通路ID Pathway ID
代谢途径Metabolic pathways	4 584	32	ko01100
次生代谢物的生物合成Biosynthesis of secondary metabolites	1 897	13	ko01110
淀粉和蔗糖代谢Starch and sucrose metabolism	1 727	12	ko00500
氨基糖和核苷酸糖代谢Amino sugar and nucleotide sugar metabolism	833	6	ko00520
RNA转运RNA transport	829	6	ko03013
内质网中的蛋白质加工Protein processing in endoplasmic reticulum	570	4	ko04141
MAPK信号通路-酵母MAPK signaling pathway-yeast	530	4	ko04011
mRNA监测通路mRNA surveillance pathway	474	3	ko03015
嘌呤代谢Purine metabolism	458	3	ko00230
细胞周期-酵母Cell cycle-yeast	435	3	ko04111
剪接体Spliceosome	354	2	ko03040
赖氨酸降解Lysine degradation	337	2	ko00310
减数分裂-酵母Meiosis-yeast	321	2	ko04113
嘧啶的代谢Pyrimidine metabolism	312	2	ko00240
RNA降解RNA degradation	293	2	ko03018
ABC运输工具ABC transporters	273	2	ko02010
核糖体Ribosome	246	2	ko03010
过氧物酶体Peroxisome	230	2	ko04146
精氨酸和脯氨酸代谢Arginine and proline metabolism	229	2	ko00330
内吞作用Endocytosis	224	2	ko04144

KEGG通路 KEGG pathway	数量 Number	比例 Proportion/%	通路ID Pathway ID
代谢途径Metabolic pathways	4 584	32	ko01100
次生代谢物的生物合成Biosynthesis of secondary metabolites	1 897	13	ko01110
淀粉和蔗糖代谢Starch and sucrose metabolism	1 727	12	ko00500
氨基糖和核苷酸糖代谢Amino sugar and nucleotide sugar metabolism	833	6	ko00520
RNA转运RNA transport	829	6	ko03013
内质网中的蛋白质加工Protein processing in endoplasmic reticulum	570	4	ko04141
MAPK信号通路-酵母MAPK signaling pathway-yeast	530	4	ko04011
mRNA监测通路mRNA surveillance pathway	474	3	ko03015
嘌呤代谢Purine metabolism	458	3	ko00230
细胞周期-酵母Cell cycle-yeast	435	3	ko04111
剪接体Spliceosome	354	2	ko03040
赖氨酸降解Lysine degradation	337	2	ko00310
减数分裂-酵母Meiosis-yeast	321	2	ko04113
嘧啶的代谢Pyrimidine metabolism	312	2	ko00240
RNA降解RNA degradation	293	2	ko03018
ABC运输工具ABC transporters	273	2	ko02010
核糖体Ribosome	246	2	ko03010
过氧物酶体Peroxisome	230	2	ko04146
精氨酸和脯氨酸代谢Arginine and proline metabolism	229	2	ko00330
内吞作用Endocytosis	224	2	ko04144

COG功能描述COG function description	数量Number	比例Proportion/%
ABC型多药转运系统,ATP酶和渗透酶成分	21	24
推测的多铜氧化酶Putative multicopper oxidases	18	21
ABC-type multidrug transport system, ATPase and permease components
RNA结合蛋白(RRM域) RNA-binding proteins (RRM domain)	12	14
RTX毒素和相关的Ca²⁺结合蛋白RTX toxins and related Ca²⁺-binding proteins	11	13
NADPH:醌还原酶和相关的Zn依赖性氧化还原酶	8	9
NADPH:quinone reductase and related Zn-dependent oxidoreductases
酰胺酶A亚单位和相关的酰胺酶	4	5
Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases
超家族I DNA和RNA螺旋酶和螺旋酶亚基	2	2
Superfamily I DNA and RNA helicases and helicase subunits
参与染色质重塑的转录因子,含溴结构域	2	2
参与大肠杆菌素摄取的膜蛋白Membrane protein involved in colicin uptake	1	1
Transcription factor involved in chromatin remodeling, contains bromodomain
翻译起始因子2 (IF-2; GTPase) Translation initiation factor 2 (IF-2; GTPase)	1	1
未知Unknown	6	7

Transcriptome sequencing and analysis of potential pathogenicity-related genes in Isaria fumosorosea

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 34

Related Articles 15

Recommended Articles

Metrics

Comments

重复次数 Number of repeats	单核苷酸重复 Mono-nucleotide repeats	二核苷酸重复 Di-nucleotide repeats	三核苷酸重复 Tri-nucleotide repeats	四核苷酸重复 Quad-nucleotide repeat	五核苷酸重复 Penta-nucleotide repeats	六核苷酸重复 Hexa-nucleotide repeats	总数 Total	比例 Proportion/%
4	0	0	0	0	136	193	329	3.84
5	0	0	1 890	157	15	0	2 062	24.06
6	0	630	1 223	72	0	0	1 925	22.46
7	0	405	908	0	0	0	1 313	15.32
8	0	247	56	0	0	0	303	3.53
9	0	201	0	0	0	0	201	2.34
10	0	230	0	0	0	0	230	2.68
11	0	145	0	0	0	0	145	1.69
12	611	11	0	0	0	0	622	7.26
13	424	0	0	0	0	0	424	4.95
14	309	0	0	0	0	0	309	3.60
15	192	0	0	0	0	0	192	2.24
16	121	0	0	0	0	0	121	1.41
17	79	0	0	0	0	0	79	0.92
18	51	0	0	0	0	0	51	0.59
19	84	0	0	0	0	0	84	0.98
20	62	0	0	0	0	0	62	0.72
21	64	0	0	0	0	0	64	0.75
22	35	0	0	0	0	0	35	0.41
23	18	0	0	0	0	0	18	0.21
24	3	0	0	0	0	0	3	0.03
总数Total	2,053	1,869	4,077	229	151	193	8 572
百分比	23.95	21.80	47.56	2.67	1.76	2.25		100
Percentage/%

重复类型 Repeat type	重复基序 Repeat motif	数量 Number	发生频率 Frequency/%
单核苷酸Mono	A/T	1 810	88.16
	C/G	243	11.84
小计Subtotal		2 053
二核苷酸Di	AC/GT	667	35.69
	AG/CT	1043	55.81
	AT/AT	64	3.42
	CG/CG	95	5.08
小计Subtotal		1 869
三核苷酸Tri	CCG/CGG	1159	28.43
	AGC/CTG	794	19.48
	AAG/CTT	536	13.15
	ACG/CGT	473	11.60
	AGG/CCT	423	10.38
	ACC/GGT	390	9.57
其他Others		302	7.41
小计Subtotal		4 077
四核苷酸Tetra	AAAG/CTTT	34	14.85
	AGGC/CCTG	33	14.41
	ACAG/CTGT	30	13.10
	ATCC/ATGG	29	12.66
	ACCT/AGGT	21	9.17
	AGCC/CTGG	20	8.73
	ACTC/AGTG	19	8.30
其他Others		43	18.78
小计Subtotal		229
五核苷酸Penta	AAAAG/CTTTT	34	22.52
	AAAAT/ATTTT	20	13.25
	AGAGG/CCTCT	15	9.93
	AGGGC/CCCTG	12	7.95
	AGCCC/CTGGG	6	3.97
	AGGCG/CCTCG	5	3.31
其他Others		59	39.07
小计Subtotal		151
六核苷酸Hexa	ACGGCG/CCGTCG	16	8.04
	AGCGGC/CCGCTG	9	4.52
	ACTGCC/AGTGGC	7	3.52
	ACCGGC/CCGGTG	6	3.02
	ACGCCC/CGTGGG	6	3.02
	AGCCGG/CCGGCT	6	3.02
其他Others		149	74.87
小计Subtotal		199

[1]	ZHANG Jun, ZHANG Bo, HU Bibo, LIU Jingliang, ZHANG Xiaoyu, LI Chunyang, XIONG Shengting, GUO Binbin, WANG Xiucun, MA Chao. Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1825-1839.
[2]	PEI Huimin, WU Mingming, ZHAI Rongrong, YE Jing, JIN Yue, ZHU Yi, HOU Jianjun, ZHU Guofu, YE Shenghai. Research progress on gene function and breeding of low-cadmium rice cultivars [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 2012-2020.
[3]	CONG Jianmin, HUANG Weijian, YIN Xinxing, LIU Jinping, ZHAO Yong. Effect of 5-azacytosine on somatic embryo-induced DNA methylation in Fraxinus mandschurica [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1648-1657.
[4]	WANG Xiaohui, JIA Sainan, FENG Jiayu, YIN Xinyue, LIU Zixuan, LIU Wenjie, ZHAO Shuaiying, WANG Shujing, TANG Yuehui. Cloning and function analysis of JcMBY27 gene from Jatropha curcas [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1658-1665.
[5]	LI Yujing, HUANG Qianru, ZHANG Aidong, WU Xuexia, ZHU Dongxing, XIAO Kai. Function of the SmMYB13 gene in drought stress response in eggplant (Solanum melongena L.) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1666-1679.
[6]	MIAO Bailing, CHEN Juanjuan, LI Liangjie, CHU Zongli, DONG Xiangxiang. The function of CchABCG5 gene in Camellia chekiangoleosa Hu [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1407-1416.
[7]	REN Jindong, CHEN Honglin, NIU Baolong, XU Xiaojun, LOU Bao. Mining new housekeeping genes of Macrobrachium rosenbergii based on transcriptome analysis [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1424-1429.
[8]	QI Yingji, LIU Shuya, LAN Hanyun, WANG Xuemei, SUN Tiantian, LI Shuhan, LI Qiunan, HUANG Xiaoli, GENG Yi, CHEN Defang, OUYANG Ping. Isolation, identification and genome-wide analysis of Spring viremia of carp virus in Sichuan Province of China [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1430-1440.
[9]	HONG Xia, LU Jilai, QI Huijuan, CHEN Xiaoshang. Genetic diversity analysis and core collection construction of ginger (Zingiber officinale Rosc.) germplasm accessions [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1233-1243.
[10]	TAO Peng, DING Xiaoya, YUE Zhichen, ZHAO Yanting, LEI Juanli, HU Qizan, ZANG Yunxiang, LI Biyuan. Genome-wide association study and candidate gene prediction of pectin content of pumpkin (Cucurbita moschata) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1244-1251.
[11]	WANG Yidi, WANG Jinglei, HU Tianhua, XU Yunmin, BAO Chonglai. Development of molecular markers for clubroot resistance and their application in Brassicaceae breeding [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1272-1284.
[12]	YI Ming, SUN Hong, SHEN Qi, TANG Jiangwu. Research progress on ectopic fermentation system in treatment of fecal residue and waste water of livestock and poultry [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1390-1396.
[13]	MA Jing'e, WU Siqi, WAN Shumin, XIONG Xinwei, XU Jiguo, XU Jing, RAO Yousheng, ZHOU Min. Proteomic study of large and small testes in Ningdu Yellow chicken based on tandem mass tags(TMT) technology [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 1029-1044.
[14]	PEI Damei, ZHAO Hongping, WANG Long, LI Huaxin, ZHAO Zhi, XIAO Lu. Mixed genetic model analysis of major gene + polygene of branch angle in Brassica rapa L. [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 745-753.
[15]	NI Zheng, LU Lei, ZHU Yinchu, CHEN Liu, LI Xinji, YE Weicheng, YUN Tao, HUA Jionggang, FU Yuan, ZHANG Cun. Epidemiological investigation and evaluation of purification strategies for avian leukosis in local chicken breeds in Zhejiang Province of China [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 790-799.