Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

doi:10.3969/j.issn.1004-1524.20221657

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (11): 2533-2542.DOI: 10.3969/j.issn.1004-1524.20221657

• Animal Science • Previous Articles Next Articles

Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

LI Hongyi(), ZHOU Runsheng, LIANG Xiaoling, ZHANG Chuyue, LYU Qixin, YANG Changhua, ZHANG Mao^*()

Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512000, Guangdong, China

Received:2022-11-19 Online:2023-11-25 Published:2023-12-04

Abstract

Abstract:

To study the effect of calcium and phosphorus levels in diet on growth performance and liver metabolism related genes in Magang geese, a total of forty eight Magang geese of 50-day-old were randomly divided into three groups, with male and female in half. Each group had two replicates with eight geese per replicate, every two geese were kept in one cage. On the basis of the same diet, 0%, 0.15% and 0.3% calcium hydrogen phosphate were added respectively, each group was pre-fed for five days and fed for twenty-eight days, during which the daily feed intake was recorded, fecal samples and blood samples were collected regularly, and the content of calcium and phosphorus in geese samples were determined. At the end of the experiment, eight Magang geese were randomly selected from each group for slaughter and the slaughter performance and phosphorylase activity were measured. The liver tissue of each geese were collected for transcriptome sequencing, fluorescence quantitative PCR and enzyme activity detection. The results showed that the feed intake of control group was significantly higher than that of the other two groups (P<0.05). The average daily gain and carcass traits were significantly higher than that of 0.15% level group, with no significant difference from 0.3% level group (P >0.05). In addition, there was no significant difference in the content of calcium and phosphorus in blood among the three groups (P>0.05), but the content of fecal calcium increased with the increase of calcium and phosphorus level (P<0.05). Transcriptome sequencing results showed that there was less difference between control group and 0.15% level group, but more significant difference between control group and 0.3% level group. And most of the differently expressed genes were enriched in sugar metabolism pathway, which was consistent with the quantitative PCR results. Also the activity of phosphorylases, the glucose metabolism-related enzyme, decreased with the increase of calcium and phosphorus levels (P<0.05). In a word, the level of calcium and phosphorus in the diet will affect the food intake of Magang geese, and it will also affect the sugar and lipid metabolism in the liver.

Key words: Magang goose, calcium and phosphorus level, growth performance, gene expression, metabolism

CLC Number:

S835

LI Hongyi, ZHOU Runsheng, LIANG Xiaoling, ZHANG Chuyue, LYU Qixin, YANG Changhua, ZHANG Mao. Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression[J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2533-2542.

Figures/Tables 10

References 21

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日粮 Dietary	比例 Proportion/ %	营养素 Nutrient	营养水平 Nutritional level
豆粕 Soybean meal, CP 43%	4.50	代谢能 Metabolic energy/ (MJ·kg^-1)	11.37
皮大麦 Leather barley	7.00	粗蛋白含量 Crude protein content/%	10.31
小麦 Wheat	10.00	粗脂肪含量 Crude fat content/%	3.28
碎米 Broken rice	50.00	粗纤维含量 Crude fiber content/%	7.51
米糠 Rice bran	15.00	钙含量 Calcium content/%	0.26
统糠 Series chaff 预混料Premix	12.00 1.50	有效磷含量 Available phosphorus content/%	0.24
合计Total	100.00

日粮 Dietary	比例 Proportion/ %	营养素 Nutrient	营养水平 Nutritional level
豆粕 Soybean meal, CP 43%	4.50	代谢能 Metabolic energy/ (MJ·kg^-1)	11.37
皮大麦 Leather barley	7.00	粗蛋白含量 Crude protein content/%	10.31
小麦 Wheat	10.00	粗脂肪含量 Crude fat content/%	3.28
碎米 Broken rice	50.00	粗纤维含量 Crude fiber content/%	7.51
米糠 Rice bran	15.00	钙含量 Calcium content/%	0.26
统糠 Series chaff 预混料Premix	12.00 1.50	有效磷含量 Available phosphorus content/%	0.24
合计Total	100.00

处理 Treatment	平均日增重 Average daily gain/g	耗料增重比 Feed/gain ratio
对照组Control group	44.64±16.17 a	6.09±2.56
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	27.09±6.43 b	6.33±1.37
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	34.09±15.72 ab	6.02±2.89

处理 Treatment	平均日增重 Average daily gain/g	耗料增重比 Feed/gain ratio
对照组Control group	44.64±16.17 a	6.09±2.56
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	27.09±6.43 b	6.33±1.37
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	34.09±15.72 ab	6.02±2.89

处理 Treatment	宰前活重 Weight	半净膛重 Semi-eviscerated weight	全净膛重 Eviscerated weight
对照组Control group	7.58±1.03 a	3.33±0.37 a	2.88±0.24 a
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	6.29±0.63 b	2.74±0.17 b	2.39±0.12 b
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	7.04±0.73 ab	3.12±0.23 ab	2.72±0.17 ab

Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

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处理 Treatment	粪磷含量 Content of calcium in feces	粪钙含量 Content of phosphorus in feces	血钙含量 Blood calcium concentration	血磷含量 Blood phosphorus concentration
对照组Control group	5.48±1.31	3.34±1.41 b	2.92±0.26	4.35±1.68
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	6.13±1.09	4.12±1.64 b	3.10±0.29	4.81±1.91
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	5.10±1.85	7.23±3.58 a	3.13±0.28	4.33±1.08

处理 Treatment	肝脏中GP活性 GP activity in liver/ (ng·mL^-1·g^-1)
对照组Control group	33.03±7.19 a
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	24.13±3.97 b
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	20.32±1.72 b

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