Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry

doi:10.3969/j.issn.1004-1524.20240087

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (3): 622-633.DOI: 10.3969/j.issn.1004-1524.20240087

• Food Science • Previous Articles Next Articles

Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry

HAN Yanchao¹(), CHEN Huizhi¹^,², NIU Ben¹^,², ZHANG Xiaoshuan³, HAN Shuren⁴, WANG Xiaoyan¹, WANG Guannan¹, LIU Ruiling²^,^*(), GAO Haiyan¹^,²^,^*()

1. Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Key Laboratory of Fruit and Vegetable Preservation and Processing in China’s Light Industry, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Key Laboratory of Fruit Postharvest Treatment of the Ministry of Agriculture and Rural Affairs, Hangzhou 310021, China
3. College of Engineering, China Agricultural University, Beijing 100083, China
4. Xianfeng Fruit Co., Ltd., Hangzhou 310000, China

Received:2024-01-21 Online:2024-03-25 Published:2024-04-09

Abstract

Abstract:

Blueberry is rich in bioactive substances such as anthocyanin and has high nutritional value. However, logistics vibration damage can accelerate the loss of nutrients such as blueberry anthocyanin and reduce nutritional quality. This article took the blueberry variety Lanmeiren as the experimental material and studied the effect of vibration stress on the anthocyanin components, metabolic enzymes activities, and genes expression of blueberry by simulating logistics vibration. The research results showed that blueberry contained 10 types of anthocyanin monomers, among which the content of quercetin-3-arabinose was the highest. In the early stage of blueberry storage, vibration stress accelerated the accumulation of anthocyanin, significantly increased the activities of phenylalanine ammonia lyase (PAL) and flavonoid glycosyltransferase (UFGT), and induced the expression of genes related to anthocyanin synthesis, such as VcPAL1, VcDFR2, VcCHI1, and VcUFGT. In the later stage of storage, the activities of PAL, chalcone isomerase (CHI), dihydroflavonol reductase (DFR), and UFGT in the vibration stress group were significantly lower than those in the control group. Meanwhile, vibration stress also delayed the decrease in the activity of enzymes related to anthocyanin degradation, such as peroxidase (POD), polyphenol oxidase (PPO) and anthocyanin-β-glucosidase, inhibited the expression of genes related to anthocyanin synthesis, and promoted the expression of genes related to anthocyanin degradation, such as VcPOD1, VcPOD2, VcPOD3, and VcPPO1. In summary, vibration stress accelerated the accumulation of anthocyanin in blueberry during early storage by increasing the activity of anthocyanin synthase and the expression of related enzyme genes. In the later stage of storage, vibration stress promoted the degradation of anthocyanin by delaying the decrease of anthocyanin degrading enzymes activities and related enzyme genes expression. The research results provided a theoretical basis for regulating the metabolism of blueberry anthocyanin.

Key words: blueberry, anthocyanin, enzyme, gene

CLC Number:

HAN Yanchao, CHEN Huizhi, NIU Ben, ZHANG Xiaoshuan, HAN Shuren, WANG Xiaoyan, WANG Guannan, LIU Ruiling, GAO Haiyan. Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry[J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 622-633.

Figures/Tables 7

References 31

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引物名称 Primer name	上游引物序列 Sequence of forward primer(5'-3')	下游引物序列 Sequence of reverse primer(5'-3')
VcPAL1	TCATGTCCAAAGTGCTGAGC	AACCAAGTGGCACTCATGAG
VcPAL2	GTTCGCATCAACACCCTCCT	GGCCCTACCGCTTTTGAGTT
VcCHI1	CAGGCAACTCCATTCTTTTC	TTCTCTATGACTGCATTCCC
VcCHI2	GCCCTTATTTCTGCTCCAGTTG	CTCTAGCTGCACACCGTACT
VcDFR1	AACCTAACGCTGTGGAAGGC	ATACTCCGACGCAACCTTCA
VcDFR2	AGAAAGCAGCATGGGAAGCA	GCTTGGTGGGAATGTAGGCA
VcDFR3	CGAGCAACCGTTCGCGATCCA	AGGTCCGCCTTCCACAGCGT
VcUFGT	AGTTTGCTTTGAAGGCTGTTG	ATGTGCTGGTGTGCATTTG
VcPOD1	ACGTTGCTTCAAAATGTGGCTT	TCCTTGAGTTTTGTACTTCTCGTAG
VcPOD2	TGCTGGTGTTGTTGCAGTTG	CGCCCTTCCTTGGGAGAAAT
VcPOD3	CTGGAGCCCATCAAGGAACA	TCCATGGGACTCTGGATGGA
VcPPO1	GCCGACTTTTAAGCCACGGA	GCTTGTCAGGGTGAAGGTGA
VcPPO2	GAGATCCTCCAACGACTCACA	AGCAGGTTTCAGTGCCCAA
VcGAPDH	ACTACCATCCACTCTATCACCG	AACACCTTACCAACAGCCTTG

引物名称 Primer name	上游引物序列 Sequence of forward primer(5'-3')	下游引物序列 Sequence of reverse primer(5'-3')
VcPAL1	TCATGTCCAAAGTGCTGAGC	AACCAAGTGGCACTCATGAG
VcPAL2	GTTCGCATCAACACCCTCCT	GGCCCTACCGCTTTTGAGTT
VcCHI1	CAGGCAACTCCATTCTTTTC	TTCTCTATGACTGCATTCCC
VcCHI2	GCCCTTATTTCTGCTCCAGTTG	CTCTAGCTGCACACCGTACT
VcDFR1	AACCTAACGCTGTGGAAGGC	ATACTCCGACGCAACCTTCA
VcDFR2	AGAAAGCAGCATGGGAAGCA	GCTTGGTGGGAATGTAGGCA
VcDFR3	CGAGCAACCGTTCGCGATCCA	AGGTCCGCCTTCCACAGCGT
VcUFGT	AGTTTGCTTTGAAGGCTGTTG	ATGTGCTGGTGTGCATTTG
VcPOD1	ACGTTGCTTCAAAATGTGGCTT	TCCTTGAGTTTTGTACTTCTCGTAG
VcPOD2	TGCTGGTGTTGTTGCAGTTG	CGCCCTTCCTTGGGAGAAAT
VcPOD3	CTGGAGCCCATCAAGGAACA	TCCATGGGACTCTGGATGGA
VcPPO1	GCCGACTTTTAAGCCACGGA	GCTTGTCAGGGTGAAGGTGA
VcPPO2	GAGATCCTCCAACGACTCACA	AGCAGGTTTCAGTGCCCAA
VcGAPDH	ACTACCATCCACTCTATCACCG	AACACCTTACCAACAGCCTTG

花色苷组分 Anthocyanin components	0 d		4 d		8 d		12 d		16 d		20 d		24 d
花色苷组分 Anthocyanin components	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h
飞燕草素-3-半乳糖苷 Delphinidin-3-galactoside	644.68 ±6.78	754.65 ±21.41^*	649.76 ±22.09	827.79 ±9.08^*	646.84 ±38.42	881.23 ±38.42^*	721.09 ±2.70	717.34 ±18.55	909 ±9.63	562.41 ±20.76^*	919.85 ±22.46	473.84 ±17.55^*	508.62 ±38.78	303.85 ±32.33^*
矢车菊素-3-半乳糖苷 Cyanin-3-galactoside	362.81 ±15.10	585.98 ±22.22^*	372.35 ±11.89	649.50 ±15.58^*	380.73 ±18.30	693.26 ±15.22^*	391.67 ±12.80	571.28 ±25.91^*	699.28 ±5.80	416.17 ±12.63^*	494.29 ±23.58	304.06 ±7.57^*	188.51 ±18.78	122.16 ±2.96^*
飞燕草素-3-阿拉伯糖苷 Delphinidin-3-arabinose glycoside	376.21 ±4.53	650.37 ±10.29^*	410.91 ±14.91	517.54 ±19.38^*	456.91 ±9.40	539.39 ±8.58^*	569.62 ±15.54	473.08 ±13.22^*	612.00 ±13.64	370.01 ±19.06^*	647.95 ±25.27	258.88 ±13.46^*	313.97 ±7.50	119.43 ±18.55^*
矮牵牛素-3-半乳糖苷 Petunionin-3-galactoside	41.35 ±2.21	47.58 ±1.49^*	46.95 ±4.58	54.51 ±2.52^*	52.01 ±2.25	68.03 ±2.32^*	58.53 ±2.69	42.68 ±4.19^*	67.00 ±5.85	46.52 ±1.62^*	51.29 ±5.42	40.52 ±1.69^*	41.17 ±1.19	29.41 ±1.41^*
矮牵牛素-3-葡萄糖苷 Petunionin-3-glucoside	67.11 ±4.66	82.82 ±12.23	71.22 ±6.19	92.63 ±11.53^*	75.35 ±6.53	141.45 ±4.44^*	116.59 ±9.60	79.17 ±9.75^*	129.36 ±5.51	64.73 ±5.10^*	100.67 ±8.54	49.39 ±6.57^*	91.01 ±9.45	35.66 ±7.12^*
芍药素-3-葡萄糖苷 Paeoniflorin-3-glucoside	7.95 ±1.50	14.99 ±1.26^*	9.10 ±1.42	19.93 ±1.48^*	8.36 ±3.78	25.66 ±0.64^*	10.68 ±1.13	17.73 ±1.22^*	12.58 ±1.73	9.76 ±0.86^*	18.75 ±2.20	5.51 ±0.64^*	22.25 ±12.29	1.78 ±0.38^*
矮牵牛素-3-阿拉伯糖苷 Petunionin-3-arabinose glycoside	62.76 ±8.64	97.03 ±8.33^*	107.17 ±8.73	102.73 ±2.02	119.68 ±4.61	135.12 ±10.81	144.28 ±2.73	97.43 ±3.81^*	99.20 ±5.61	67.28 ±3.84^*	55.45 ±6.33	38.42 ±6.48^*	37.96 ±5.71	21.56 ±3.01^*
锦葵素-3-半乳糖苷 Malvacin-3-galactoside	817 ±27.81	962.59 ±34.18^*	851.76 ±11.51	856.38 ±26.93^*	912.44 ±14.96	774.60 ±24.83^*	932.58 ±16.85	599.27 ±6.50^*	714.22 ±79.10	534.08 ±34.81	534.53 ±23.99	365.28 ±27.98^*	414.20 ±16.71	189.87 ±24.71^*
锦葵素-3-葡萄糖苷 Malvacin-3-glucoside	36.26 ±2.54	39.03 ±2.02	37.26 ±1.28	38.27 ±0.95	41.26 ±1.81	44.55 ±2.50	41.44 ±0.80	35.36 ±1.38^*	36.83 ±2.40	33.26 ±1.75	46.79 ±1.59	37.40 ±2.69^*	31.97 ±1.55	28.06 ±2.61^*
锦葵素-3-阿拉伯糖苷 Malvacin-3-arabinose glycoside	5 246.72 ±503.30	6 166.53 ±179.92^*	5 289.86 ±220.22	7 276.47 ±286.78^*	6 930.70 ±142.71	10 746.69 ±133.75^*	8 313.87 ±377.03	7 740.23 ±291.46^*	8 719.88 ±365.39	5 296.44 ±22.67^*	6 282.75 ±75.21	3 788.40 ±358.62^*	2 717.32 ±128.54	2 117.73 ±251.78^*

花色苷组分 Anthocyanin components	0 d		4 d		8 d		12 d		16 d		20 d		24 d
花色苷组分 Anthocyanin components	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h	0 h	18 h
飞燕草素-3-半乳糖苷 Delphinidin-3-galactoside	644.68 ±6.78	754.65 ±21.41^*	649.76 ±22.09	827.79 ±9.08^*	646.84 ±38.42	881.23 ±38.42^*	721.09 ±2.70	717.34 ±18.55	909 ±9.63	562.41 ±20.76^*	919.85 ±22.46	473.84 ±17.55^*	508.62 ±38.78	303.85 ±32.33^*
矢车菊素-3-半乳糖苷 Cyanin-3-galactoside	362.81 ±15.10	585.98 ±22.22^*	372.35 ±11.89	649.50 ±15.58^*	380.73 ±18.30	693.26 ±15.22^*	391.67 ±12.80	571.28 ±25.91^*	699.28 ±5.80	416.17 ±12.63^*	494.29 ±23.58	304.06 ±7.57^*	188.51 ±18.78	122.16 ±2.96^*
飞燕草素-3-阿拉伯糖苷 Delphinidin-3-arabinose glycoside	376.21 ±4.53	650.37 ±10.29^*	410.91 ±14.91	517.54 ±19.38^*	456.91 ±9.40	539.39 ±8.58^*	569.62 ±15.54	473.08 ±13.22^*	612.00 ±13.64	370.01 ±19.06^*	647.95 ±25.27	258.88 ±13.46^*	313.97 ±7.50	119.43 ±18.55^*
矮牵牛素-3-半乳糖苷 Petunionin-3-galactoside	41.35 ±2.21	47.58 ±1.49^*	46.95 ±4.58	54.51 ±2.52^*	52.01 ±2.25	68.03 ±2.32^*	58.53 ±2.69	42.68 ±4.19^*	67.00 ±5.85	46.52 ±1.62^*	51.29 ±5.42	40.52 ±1.69^*	41.17 ±1.19	29.41 ±1.41^*
矮牵牛素-3-葡萄糖苷 Petunionin-3-glucoside	67.11 ±4.66	82.82 ±12.23	71.22 ±6.19	92.63 ±11.53^*	75.35 ±6.53	141.45 ±4.44^*	116.59 ±9.60	79.17 ±9.75^*	129.36 ±5.51	64.73 ±5.10^*	100.67 ±8.54	49.39 ±6.57^*	91.01 ±9.45	35.66 ±7.12^*
芍药素-3-葡萄糖苷 Paeoniflorin-3-glucoside	7.95 ±1.50	14.99 ±1.26^*	9.10 ±1.42	19.93 ±1.48^*	8.36 ±3.78	25.66 ±0.64^*	10.68 ±1.13	17.73 ±1.22^*	12.58 ±1.73	9.76 ±0.86^*	18.75 ±2.20	5.51 ±0.64^*	22.25 ±12.29	1.78 ±0.38^*
矮牵牛素-3-阿拉伯糖苷 Petunionin-3-arabinose glycoside	62.76 ±8.64	97.03 ±8.33^*	107.17 ±8.73	102.73 ±2.02	119.68 ±4.61	135.12 ±10.81	144.28 ±2.73	97.43 ±3.81^*	99.20 ±5.61	67.28 ±3.84^*	55.45 ±6.33	38.42 ±6.48^*	37.96 ±5.71	21.56 ±3.01^*
锦葵素-3-半乳糖苷 Malvacin-3-galactoside	817 ±27.81	962.59 ±34.18^*	851.76 ±11.51	856.38 ±26.93^*	912.44 ±14.96	774.60 ±24.83^*	932.58 ±16.85	599.27 ±6.50^*	714.22 ±79.10	534.08 ±34.81	534.53 ±23.99	365.28 ±27.98^*	414.20 ±16.71	189.87 ±24.71^*
锦葵素-3-葡萄糖苷 Malvacin-3-glucoside	36.26 ±2.54	39.03 ±2.02	37.26 ±1.28	38.27 ±0.95	41.26 ±1.81	44.55 ±2.50	41.44 ±0.80	35.36 ±1.38^*	36.83 ±2.40	33.26 ±1.75	46.79 ±1.59	37.40 ±2.69^*	31.97 ±1.55	28.06 ±2.61^*
锦葵素-3-阿拉伯糖苷 Malvacin-3-arabinose glycoside	5 246.72 ±503.30	6 166.53 ±179.92^*	5 289.86 ±220.22	7 276.47 ±286.78^*	6 930.70 ±142.71	10 746.69 ±133.75^*	8 313.87 ±377.03	7 740.23 ±291.46^*	8 719.88 ±365.39	5 296.44 ±22.67^*	6 282.75 ±75.21	3 788.40 ±358.62^*	2 717.32 ±128.54	2 117.73 ±251.78^*

Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry

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