Development of SNP and InDel markers in radish and their association with phenotypic characters

doi:10.3969/j.issn.1004-1524.20230663

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (5): 1055-1066.DOI: 10.3969/j.issn.1004-1524.20230663

• Horticultural Science • Previous Articles Next Articles

Development of SNP and InDel markers in radish and their association with phenotypic characters

LI Yadong¹^,²(), LUO Xiaobo³, PENG Xiao¹^,², YANG Guangqian¹^,², JIN Yueyue¹^,², ZU Guidong⁴, TIAN Huan⁵, ZHANG Wanping¹^,²^,^*()

1. College of Agriculture, Guizhou University, Guiyang 550025, China
2. Vegetable Research Institute, Guizhou University, Guiyang 550025, China
3. Guizhou Academy of Agricultural Sciences, Guiyang 550000, China
4. Bureau of Agriculture and Rural Affairs of Weining County, Weining 553100, Guizhou, China
5. Guizhou Modern Seed Industry Group Co., Ltd., Guiyang 550081, China

Received:2023-05-20 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

In order to explore the molecular markers associated with significant phenotypic traits of radish, we identified 14 phenotypic data of 60 radish samples, and screened out 29 molecular markers with polymorphism (12 SNP markers and 17 InDel markers) for genetic diversity analysis. The general linear mode (GLM) of TASSEL5.0 software was used for correlation analysis of 14 phenotypic data of radish. The results showed that the number of alleles (Na) of 29 molecular markers ranged from 2 to 3, with an average of 2.14. The frequency of major alleles (MAF) ranged from 0.53 to 0.94, with an average of 0.68. The expected heterozygosity (He) for each marker ranged from 0.12 to 0.93, with an average of 0.63. The polymorphism information content (PIC) values of each locus ranged from 0.11 to 0.37, with an average value of 0.32. Among the 12 phenotypic traits, 17 significantly correlated molecular marker sites (7 SNP markers and 10 InDel markers) were associated (P≤0.01), and the contribution rate was 7.24%-23.25%.

Key words: radish, SNP, InDel, cluster analysis, association analysis

CLC Number:

S631.1

LI Yadong, LUO Xiaobo, PENG Xiao, YANG Guangqian, JIN Yueyue, ZU Guidong, TIAN Huan, ZHANG Wanping. Development of SNP and InDel markers in radish and their association with phenotypic characters[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1055-1066.

Figures/Tables 10

References 24

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序号 No.	品种 Variety	序号 No.	品种 Variety	序号 No.	品种 Variety	序号 No.	品种 Variety
1	彩虹一号 Caihong 1	16	盛青三号 Shengqing No.3	31	君川玉洁 Junchunyujie	46	脆宝萝卜 Cuibaoluobo
2	紫美人F₁ Zimeiren F₁	17	德高清泉 Degaoqingquan	32	玉娇龙 Yujiaolong	47	德蔬秋玉791 Deshuqiuyu 791
3	首尔紫脆 Shouerzicui	18	大韩白春 Dahanbaichun	33	珍白玉 Zhenbaiyu	48	青云萝卜 Qingyunluobo
4	笑脸 Xiaolian	19	新西兰短叶13号 Xinxilanduanye 13	34	金天厚971 Jintianhou 971	49	冰糖脆里外青 Bingtangcuiliwaiqing
5	手指萝卜 Shouzhiluobo	20	WN1	35	金天厚906 Jintianhou 906	50	露头青萝卜 Lutouqingluobo
6	京红30 Jinghong 30	21	WN2	36	金天厚949 Jintianhou 949	51	渝星翠雪 Yuxingcuixue
7	徐州大红袍萝卜 Xuzhoudahongpaoluobo	22	WN3	37	金天厚112 Jintianhou 112	52	盛青一号 Shengqing No.1
8	盛农串心红 Shengnongchuanxinhong	23	慕田红福二号 Mutianhongfu No.2	38	金天厚948 Jintianhou 948	53	富莹一号 Fuying No.1
9	樱桃萝卜萨丁 Yingtaoluobosading	24	地增宝2号 Dizengbao 2	39	半夏萝卜 Banxialuobo	54	凯撒203 Kaisa 203
10	红心萝卜 Hongxinluobo	25	日本大根 Ribendagen	40	胭脂白玉 Yanzhibaiyu	55	GZ1号
11	凤梨水果萝卜 Fenglishuiguoluobo	26	春不老 Chunbulao	41	玉露218 Yulu218	56	GZ2号
12	江南圆白 Yunnanyuanbai	27	金天厚R2008 Jintianhou R2008	42	春白玉 Chunbaiyu	57	GDE
13	扬州圆白 Yangzhouyuanbai	28	地增宝4号 Dizengbao 4	43	德高秋玉 Degaoqiuyu	58	GDL
14	韩国大根 Hanguodagen	29	美龙123 Meilong 123	44	白玉2号 Baiyu 2	59	YZH
15	中萝1号 Zhongluo 1	30	上将9号 Shangjiang 9	45	郫县黑叶春不老 Pixianheiyechunbulao	60	XHT

序号 No.	品种 Variety	序号 No.	品种 Variety	序号 No.	品种 Variety	序号 No.	品种 Variety
1	彩虹一号 Caihong 1	16	盛青三号 Shengqing No.3	31	君川玉洁 Junchunyujie	46	脆宝萝卜 Cuibaoluobo
2	紫美人F₁ Zimeiren F₁	17	德高清泉 Degaoqingquan	32	玉娇龙 Yujiaolong	47	德蔬秋玉791 Deshuqiuyu 791
3	首尔紫脆 Shouerzicui	18	大韩白春 Dahanbaichun	33	珍白玉 Zhenbaiyu	48	青云萝卜 Qingyunluobo
4	笑脸 Xiaolian	19	新西兰短叶13号 Xinxilanduanye 13	34	金天厚971 Jintianhou 971	49	冰糖脆里外青 Bingtangcuiliwaiqing
5	手指萝卜 Shouzhiluobo	20	WN1	35	金天厚906 Jintianhou 906	50	露头青萝卜 Lutouqingluobo
6	京红30 Jinghong 30	21	WN2	36	金天厚949 Jintianhou 949	51	渝星翠雪 Yuxingcuixue
7	徐州大红袍萝卜 Xuzhoudahongpaoluobo	22	WN3	37	金天厚112 Jintianhou 112	52	盛青一号 Shengqing No.1
8	盛农串心红 Shengnongchuanxinhong	23	慕田红福二号 Mutianhongfu No.2	38	金天厚948 Jintianhou 948	53	富莹一号 Fuying No.1
9	樱桃萝卜萨丁 Yingtaoluobosading	24	地增宝2号 Dizengbao 2	39	半夏萝卜 Banxialuobo	54	凯撒203 Kaisa 203
10	红心萝卜 Hongxinluobo	25	日本大根 Ribendagen	40	胭脂白玉 Yanzhibaiyu	55	GZ1号
11	凤梨水果萝卜 Fenglishuiguoluobo	26	春不老 Chunbulao	41	玉露218 Yulu218	56	GZ2号
12	江南圆白 Yunnanyuanbai	27	金天厚R2008 Jintianhou R2008	42	春白玉 Chunbaiyu	57	GDE
13	扬州圆白 Yangzhouyuanbai	28	地增宝4号 Dizengbao 4	43	德高秋玉 Degaoqiuyu	58	GDL
14	韩国大根 Hanguodagen	29	美龙123 Meilong 123	44	白玉2号 Baiyu 2	59	YZH
15	中萝1号 Zhongluo 1	30	上将9号 Shangjiang 9	45	郫县黑叶春不老 Pixianheiyechunbulao	60	XHT

性状 Trait	赋值 Assignment
叶型	1,板叶;2,花叶
Leaf profile	1, Plate leaf; 2, Flowers and leaves
叶形	1,卵圆;2,长卵圆;3,倒卵圆;4,长倒卵圆;5,椭圆;6,长椭圆;7,批针
Leaf shape	1, Oval; 2, Long oval; 3, Inverted oval; 4, Long oblong oval; 5, Ellipse; 6, Long ellipse; 7, Batches of needles
叶尖	1,锐尖;2,尖;3,圆;4,阔圆
Leaf opex	1, Sharp point; 2, Tip; 3, Circles; 4, Wide round
叶色	1,黄绿;2,浅绿;3,绿;4,深绿
Leaf colour	1, Yellow green; 2, Light green; 3, Green; 4, Dark green
叶脉色	1,黄绿;2,浅绿;3,绿;4,红;5,浅紫;6,紫
Color of leaf veins	1, Yellow green; 2, Light green; 3, Green; 4, Red; 5, Light purple; 6, Purple
叶面刺毛Leaf seta	0,无;1,少; 2,中;3,多;0, None; 1, Less; 2, Medium; 3, More
叶缘	0,无裂刻;1,全缘;2,波状;3,锯齿
Leaf margin	0, No cracking; 1, Whole margin; 2, Wave-like; 3, Sawtooth
叶裂刻	1,浅裂;2,深裂;3,全裂
Leaf crack	1, Lobulated; 2, Deep cleft; 3, Complete cleavage
肉质根侧根分布		0,无;1,少;2,中;3,多
Lateral root distribution		0, None; 1, Less; 2, Medium; 3, More
肉质根表面光滑度		1,光滑;2,中等;3,粗糙
Smoothness of the fleshy		1, Smooth; 2, Medium; 3, Rough
root surface
根形Root form		1,长圆柱;2,短圆柱;3,长弯号角;4,短弯号角;5,长圆锥;6,短圆锥;7,倒长圆锥;8,倒短圆锥;9,卵圆;10,扁圆;11,近圆;12,梨形;13,纺锤形;14,高圆台;15,矮圆台
		1, Long cylinder; 2, Short cylinder; 3, Long horn; 4, Short horn; 5, Long cone; 6, Short cone; 7, Inverted long cone; 8, Inverted short cone; 9, Oval; 10, Oblong; 11, Near round; 12, Pear shaped; 13, Spindle shape; 14, High round table; 15, Low round table
肉质根地上部皮色		1,白;2,白绿;3,浅绿;4,绿;5,深绿;6,粉红;7,红;8,浅紫;9,紫
Color of aboveground part		1, White; 2, White green; 3, Light green; 4, Green; 5, Dark green; 6, Pink; 7, Red; 8, Light purple; 9, Purple
of fleshy root
肉质根地下部皮色		1,白;2,浅绿;3,绿;4,粉红;5,红;6,紫
Color of underground part		1, White; 2, Light green; 3, Green; 4, Pink; 5, Red; 6, Purple
of fleshy root
肉色		1,白;2,浅绿;3,绿;4,深绿;5,粉红;6,红;7,浅紫;8,紫
Color of flesh		1, White; 2, Light green; 3, Green; 4, Dark green; 5, Pink; 6, Red; 7, Light purple; 8, Purple

性状 Trait	赋值 Assignment
叶型	1,板叶;2,花叶
Leaf profile	1, Plate leaf; 2, Flowers and leaves
叶形	1,卵圆;2,长卵圆;3,倒卵圆;4,长倒卵圆;5,椭圆;6,长椭圆;7,批针
Leaf shape	1, Oval; 2, Long oval; 3, Inverted oval; 4, Long oblong oval; 5, Ellipse; 6, Long ellipse; 7, Batches of needles
叶尖	1,锐尖;2,尖;3,圆;4,阔圆
Leaf opex	1, Sharp point; 2, Tip; 3, Circles; 4, Wide round
叶色	1,黄绿;2,浅绿;3,绿;4,深绿
Leaf colour	1, Yellow green; 2, Light green; 3, Green; 4, Dark green
叶脉色	1,黄绿;2,浅绿;3,绿;4,红;5,浅紫;6,紫
Color of leaf veins	1, Yellow green; 2, Light green; 3, Green; 4, Red; 5, Light purple; 6, Purple
叶面刺毛Leaf seta	0,无;1,少; 2,中;3,多;0, None; 1, Less; 2, Medium; 3, More
叶缘	0,无裂刻;1,全缘;2,波状;3,锯齿
Leaf margin	0, No cracking; 1, Whole margin; 2, Wave-like; 3, Sawtooth
叶裂刻	1,浅裂;2,深裂;3,全裂
Leaf crack	1, Lobulated; 2, Deep cleft; 3, Complete cleavage
肉质根侧根分布		0,无;1,少;2,中;3,多
Lateral root distribution		0, None; 1, Less; 2, Medium; 3, More
肉质根表面光滑度		1,光滑;2,中等;3,粗糙
Smoothness of the fleshy		1, Smooth; 2, Medium; 3, Rough
root surface
根形Root form		1,长圆柱;2,短圆柱;3,长弯号角;4,短弯号角;5,长圆锥;6,短圆锥;7,倒长圆锥;8,倒短圆锥;9,卵圆;10,扁圆;11,近圆;12,梨形;13,纺锤形;14,高圆台;15,矮圆台
		1, Long cylinder; 2, Short cylinder; 3, Long horn; 4, Short horn; 5, Long cone; 6, Short cone; 7, Inverted long cone; 8, Inverted short cone; 9, Oval; 10, Oblong; 11, Near round; 12, Pear shaped; 13, Spindle shape; 14, High round table; 15, Low round table
肉质根地上部皮色		1,白;2,白绿;3,浅绿;4,绿;5,深绿;6,粉红;7,红;8,浅紫;9,紫
Color of aboveground part		1, White; 2, White green; 3, Light green; 4, Green; 5, Dark green; 6, Pink; 7, Red; 8, Light purple; 9, Purple
of fleshy root
肉质根地下部皮色		1,白;2,浅绿;3,绿;4,粉红;5,红;6,紫
Color of underground part		1, White; 2, Light green; 3, Green; 4, Pink; 5, Red; 6, Purple
of fleshy root
肉色		1,白;2,浅绿;3,绿;4,深绿;5,粉红;6,红;7,浅紫;8,紫
Color of flesh		1, White; 2, Light green; 3, Green; 4, Dark green; 5, Pink; 6, Red; 7, Light purple; 8, Purple

引物名称 Primer name	染色体 Chr	位置 Position	注释的基因 Anno_ Gene	注释的位置 Anno_Pos	正向引物 Forward primer	反向引物 Reverse primer	产物大小 Product size/bp
InDelR1-2	R1	776 114	Rs001290	frameshift	TCTGTTTGCCAGTTTCGT	AAACAAGAACAGCCCCAT	123
InDelR1-3	R1	1 313 749	Rs002390	nonframeshift	GTTTCCGTCGTCACTTCT	GAAGCCAAATCACAAGTC	167
InDelR2-1	R2	2 594 367	Rs101280	intronic	TCCCTGTAAATTCTTGTC	AAGAAACAGTACCGTCAA	198
InDelR2-3	R2	9 755 367	Rs093700	upstream	GATGATTCGTGTTTCTTG	GCACTAACGAATTAGGTG	170
InDelR3-1	R3	17 365 594	Rs128600	intronic	GGCTCAATGATACCTCCC	GTCAGCCAAGGTACGACC	169
InDelR3-2	R3	22 641 607	Rs136110	intergenic	TGCAATAGTGTTGAGGAC	CGTATCCACCACTACATA	167
InDelR4-2	R4	6 629 791	Rs161140	intronic	CAAACCCTGAAAACCCAT	CTTGTGGTGCGATGCTAT	199
InDelR5-1	R5	3 652 986	Rs225420	intronic	GCTGGTATCCATTCTCCT	TAAAGTGCAAGAAGCGAG	141
InDelR5-2	R5	7 215 633	Rs231360	upstream	GAATCATTTACAGCATCA	TGGAACCGATTAAAGTGT	128
InDelR5-3	R5	11 178 127	Rs237340	UTR3	GTTGTGGGACTCTTGTGA	AGAACAAGATCAGATCCATG	200
InDelR6-3	R6	11 171 972	Rs308290	nonframeshift	TAAGGGAGACGATGAACC	GCTCTGTTTCCTGCAAAG	197
InDelR7-1	R7	5 269 877	Rs396800	UTR3	GGTTGATGGATGGGAATT	ACTCCCAAGTTCCTTTGC	176
InDelR7-3	R7	15 927 653	Rs381480	intergenic	TATGTGATTGATTGGCAT	GAAGATGTGGAAAACGAT	199
InDelR8-1	R8	5 489 898	Rs416170	UTR5	TGGGTTTGTACGTTGTTG	TCTATCAAATCCCTCGTA	135
InDelR8-2	R8	11 419 948	Rs425900	intergenic	GCATCCTGACTTGCTCTG	AGGAGGGGAGATGACAAG	133
InDelR9-1	R9	5 051 478	Rs455320	intronic	TGTCTTCTTTCGTGTCCT	CTAATGACACCCAGAAAGA	170
InDelR9-2	R9	11 215 705	Rs463920	intergenic	TTATAGGGTCTTCTTGAG	ATGTGCCTCAAAATCTGT	187

Development of SNP and InDel markers in radish and their association with phenotypic characters

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引物名称 Primer name	染色体 Chromo- some	位置 Position	注释的基因 Anno_ Gene	注释的位置 Anno_Pos	参考序列 Reference sequence	突变序列 Mutant sequence	正向引物 Forward primer	反向引物 Reverse primer	酶 Enzyme	产物大小 Product size/bp
SNP-R1-11	R1	169 238	Rs000940	Intergenic	G	A	TTGCTGAAGTGCTGTGAGT	TGCGAATGGAAGATGATG	HinfⅠ	436
SNP-R1-15	R1	183 664	Rs000900	Exonic	C	G	CAGGTTTTTGCCCCCA	ATTCCCCGGCCATTG	TaqⅠ	281
SNP-R2-2	R2	954	Rs104740	Exonic	T	C	CATGAGATGCGGAGAGAGCA	TGTCAGTCCCACCGTTGAGA	TaqⅠ	214
SNP-R2-9	R2	230 920	Rs103580	Downstream	C	G	AGCCTGAACAGAGAAGCCA	CCACCATGATCCCGAATATA	HinfⅠ	325
SNP-R2-10	R2	238 894	Rs103540	Exonic	A	T	CAGTGGCGAAGCGAAGA	CCTAACGTGCAAAGGAGGT	HinfⅠ	263
SNP-R3-2	R3	50 058	Rs105180	Intergenic	T	C	CTATCCACGAATCCCAC	GTAATCAATGCCTATGTCC	HinfⅠ	514
SNP-R3-3	R3	52 883	Rs105200	Exonic	G	A	AGAGGAGTGTTGGATGGAGT	CAGAGCGGCTATGGTGA	HinfⅠ	459
SNP-R3-10	R3	642 112	Rs105440	Intergenic	G	A	AAACTCCATCACCATCACA	GCAAAAGGAAAGCACACT	HinfⅠ	533
SNP-R4-1	R4	75 754	Rs148670	Splicing	G	C	ATGTCGTCCAAAATACCTCC	AGCACCAGCCATCAAGC	SacⅠ	417
SNP-R5-2	R5	121 387	Rs219310	Intergenic	A	G	CTGTCTTGTTTGCTATTACTCG	TATCTCAGCCTCCGTCG	HinfⅠ	406
SNP-R6-5	R6	279 865	Rs289940	Intergenic	C	G	TTTAGGATTGGGAGTGAAGAC	AATAACAAAACGGGGAAGG	TaqⅠ	390
SNP-R8-3	R8	113 322	Rs405880	Exonic	A	G	TTCAGTCCCTTGTTTAGTCTTC	GGCATGTAACGTGCATCC	BamHⅠ	472

序号 Numerical order	叶型 Leaf profile	叶形 Leaf shape	叶尖 Leaf opex	叶色 Leaf colour	叶脉色 Color of leaf veins	叶面毛刺 Leaf seta	肉质根地上部皮色 Color of aboveground part of fleshy root	叶缘 Leaf margin	叶裂刻 Leaf crack	肉质根侧根分布 Lateral root distribu- tion	肉质根表面光滑度 Smoothness of the fleshy root surface	根形 Root form	肉色 Color of flesh	肉质根地下部皮色 Color of underground part of fleshy root
1	2	6	3	3	5	2	7	2	2	1	1	9	5	5
2	2	6	2	4	5	1	7	2	2	0	2	7	6	5
3	2	6	3	4	5	1	7	2	2	0	2	13	6	5
4	2	6	4	3	1	1	7	2	2	0	2	11	5	1
5	2	6	4	3	4	1	7	2	3	0	1	1	1	5
6	1	7	1	3	4	2	7	1	1	0	1	3	1	5
7	2	6	1	4	4	1	7	3	3	3	1	13	1	5
8	1	5	3	4	2	2	4	0	1	1	2	7	5	4
9	2	6	4	4	4	2	7	2	2	0	1	2	1	5
10	2	6	2	3	4	0	7	2	2	1	1	5	6	5
11	2	6	2	4	6	1	9	3	3	2	2	7	7	6
12	1	6	2	4	3	3	1	2	2	0	1	10	1	1
13	2	6	1	3	2	0	1	3	3	1	1	9	1	1
14	2	6	3	4	3	3	3	2	2	2	2	1	1	1
15	2	6	2	4	3	2	1	3	2	3	1	13	1	1
16	2	6	2	3	3	1	4	1	3	0	2	2	3	2
17	2	6	4	4	3	1	5	2	2	1	1	13	3	2
18	2	6	4	4	2	1	3	2	2	2	1	1	1	1
19	1	3	4	4	2	0	1	1	0	3	1	3	1	1
20	1	6	2	3	5	2	9	3	3	3	1	3	1	1
21	2	6	2	3	6	1	9	2	3	3	1	1	1	6
22	2	4	2	2	2	1	1	1	2	2	2	3	1	1
23	1	6	4	4	4	2	7	2	0	2	1	5	1	5
24	2	6	1	4	2	3	3	3	3	2	1	3	1	1
25	2	6	3	3	3	1	2	2	2	2	2	7	6	4
26	2	6	4	4	3	1	4	2	2	1	1	9	2	1
27	1	4	3	1	2	1	1	2	1	1	1	9	1	1
28	2	6	3	4	2	3	1	2	2	2	2	7	1	1
29	2	6	2	4	3	2	1	2	2	2	1	13	1	1
30	2	6	1	4	2	2	1	3	3	2	1	1	1	1
31	1	4	2	4	4	0	7	2	1	3	1	13	1	4
32	2	6	4	4	2	1	2	2	2	3	1	13	1	1
33	2	6	1	4	2	0	1	3	3	3	1	1	1	1
34	1	6	4	4	2	1	4	2	0	1	1	9	1	1
35	2	6	4	4	3	2	1	2	2	2	1	3	1	1
36	2	6	4	4	2	1	1	2	3	1	1	13	1	1
37	2	6	4	4	2	1	1	2	3	2	1	13	1	1
38	2	6	2	4	2	1	1	3	3	2	1	7	1	1
39	1	6	2	3	4	2	7	2	0	2	1	7	1	4
40	2	6	2	3	2	0	1	3	3	1	1	11	1	1
41	2	6	4	4	3	3	1	1	2	2	1	5	1	1
42	2	6	2	4	3	3	1	2	2	3	1	7	1	1
43	2	6	2	4	2	1	1	3	3	3	1	13	1	1
44	2	6	4	4	3	1	1	2	2	2	1	3	1	1
45	1	4	1	3	3	1	1	3	1	2	2	13	1	1
46	2	6	2	4	2	2	5	3	3	3	1	13	1	1
47	2	6	2	4	2	2	1	3	3	2	1	3	1	1
48	2	6	2	3	2	1	4	2	2	1	1	13	3	1
49	2	6	2	4	3	1	5	3	3	1	1	13	2	1
50	2	6	2	4	2	2	1	3	3	3	1	13	1	1
51	2	6	4	3	3	2	2	1	2	2	1	2	1	1
52	2	6	4	4	3	3	1	2	2	1	2	13	1	1
53	1	4	4	4	3	2	1	2	0	2	2	13	1	1
54	1	6	2	4	2	1	1	3	0	2	1	13	1	1
55	1	6	2	4	2	2	2	3	0	1	1	13	1	1
56	2	6	2	3	4	2	7	1	2	1	1	5	7	1
57	1	6	3	3	2	2	1	3	1	1	1	11	1	1
58	1	6	2	4	2	2	1	2	1	1	1	11	1	1
59	1	4	2	3	4	2	7	3	0	1	1	10	1	5
60	1	4	4	3	4	3	7	2	0	1	1	5	4	1

序号 Numerical order	叶型 Leaf profile	叶形 Leaf shape	叶尖 Leaf opex	叶色 Leaf colour	叶脉色 Color of leaf veins	叶面毛刺 Leaf seta	肉质根地上部皮色 Color of aboveground part of fleshy root	叶缘 Leaf margin	叶裂刻 Leaf crack	肉质根侧根分布 Lateral root distribu- tion	肉质根表面光滑度 Smoothness of the fleshy root surface	根形 Root form	肉色 Color of flesh	肉质根地下部皮色 Color of underground part of fleshy root
1	2	6	3	3	5	2	7	2	2	1	1	9	5	5
2	2	6	2	4	5	1	7	2	2	0	2	7	6	5
3	2	6	3	4	5	1	7	2	2	0	2	13	6	5
4	2	6	4	3	1	1	7	2	2	0	2	11	5	1
5	2	6	4	3	4	1	7	2	3	0	1	1	1	5
6	1	7	1	3	4	2	7	1	1	0	1	3	1	5
7	2	6	1	4	4	1	7	3	3	3	1	13	1	5
8	1	5	3	4	2	2	4	0	1	1	2	7	5	4
9	2	6	4	4	4	2	7	2	2	0	1	2	1	5
10	2	6	2	3	4	0	7	2	2	1	1	5	6	5
11	2	6	2	4	6	1	9	3	3	2	2	7	7	6
12	1	6	2	4	3	3	1	2	2	0	1	10	1	1
13	2	6	1	3	2	0	1	3	3	1	1	9	1	1
14	2	6	3	4	3	3	3	2	2	2	2	1	1	1
15	2	6	2	4	3	2	1	3	2	3	1	13	1	1
16	2	6	2	3	3	1	4	1	3	0	2	2	3	2
17	2	6	4	4	3	1	5	2	2	1	1	13	3	2
18	2	6	4	4	2	1	3	2	2	2	1	1	1	1
19	1	3	4	4	2	0	1	1	0	3	1	3	1	1
20	1	6	2	3	5	2	9	3	3	3	1	3	1	1
21	2	6	2	3	6	1	9	2	3	3	1	1	1	6
22	2	4	2	2	2	1	1	1	2	2	2	3	1	1
23	1	6	4	4	4	2	7	2	0	2	1	5	1	5
24	2	6	1	4	2	3	3	3	3	2	1	3	1	1
25	2	6	3	3	3	1	2	2	2	2	2	7	6	4
26	2	6	4	4	3	1	4	2	2	1	1	9	2	1
27	1	4	3	1	2	1	1	2	1	1	1	9	1	1
28	2	6	3	4	2	3	1	2	2	2	2	7	1	1
29	2	6	2	4	3	2	1	2	2	2	1	13	1	1
30	2	6	1	4	2	2	1	3	3	2	1	1	1	1
31	1	4	2	4	4	0	7	2	1	3	1	13	1	4
32	2	6	4	4	2	1	2	2	2	3	1	13	1	1
33	2	6	1	4	2	0	1	3	3	3	1	1	1	1
34	1	6	4	4	2	1	4	2	0	1	1	9	1	1
35	2	6	4	4	3	2	1	2	2	2	1	3	1	1
36	2	6	4	4	2	1	1	2	3	1	1	13	1	1
37	2	6	4	4	2	1	1	2	3	2	1	13	1	1
38	2	6	2	4	2	1	1	3	3	2	1	7	1	1
39	1	6	2	3	4	2	7	2	0	2	1	7	1	4
40	2	6	2	3	2	0	1	3	3	1	1	11	1	1
41	2	6	4	4	3	3	1	1	2	2	1	5	1	1
42	2	6	2	4	3	3	1	2	2	3	1	7	1	1
43	2	6	2	4	2	1	1	3	3	3	1	13	1	1
44	2	6	4	4	3	1	1	2	2	2	1	3	1	1
45	1	4	1	3	3	1	1	3	1	2	2	13	1	1
46	2	6	2	4	2	2	5	3	3	3	1	13	1	1
47	2	6	2	4	2	2	1	3	3	2	1	3	1	1
48	2	6	2	3	2	1	4	2	2	1	1	13	3	1
49	2	6	2	4	3	1	5	3	3	1	1	13	2	1
50	2	6	2	4	2	2	1	3	3	3	1	13	1	1
51	2	6	4	3	3	2	2	1	2	2	1	2	1	1
52	2	6	4	4	3	3	1	2	2	1	2	13	1	1
53	1	4	4	4	3	2	1	2	0	2	2	13	1	1
54	1	6	2	4	2	1	1	3	0	2	1	13	1	1
55	1	6	2	4	2	2	2	3	0	1	1	13	1	1
56	2	6	2	3	4	2	7	1	2	1	1	5	7	1
57	1	6	3	3	2	2	1	3	1	1	1	11	1	1
58	1	6	2	4	2	2	1	2	1	1	1	11	1	1
59	1	4	2	3	4	2	7	3	0	1	1	10	1	5
60	1	4	4	3	4	3	7	2	0	1	1	5	4	1

标记类型 Marker type	R01	R02	R03	R04	R05	R06	R07	R08	R09	总数 Total
SNP	40 434	42 072	31 672	37 651	63 913	66 979	23 305	26 566	34 087	366 679
InDel	10 038	11 486	8 394	10 551	17 136	17 689	6 416	6 827	9 436	97 973

引物名称 Primer name	MAF	Na	He	PIC	引物名称 Primer name	MAF	Na	He	PIC
InDelR1-2	0.68	2	0.63	0.34	InDelR9-1	0.63	2	0.73	0.36
InDelR1-3	0.65	2	0.70	0.35	InDelR9-2	0.67	2	0.67	0.35
InDelR2-1	0.68	2	0.63	0.34	SNP-R1-11	0.58	2	0.83	0.37
InDelR2-3	0.58	2	0.85	0.37	SNP-R1-15	0.65	2	0.70	0.35
InDelR3-1	0.94	2	0.12	0.10	SNP-R2-2	0.80	2	0.40	0.27
InDelR3-2	0.68	3	0.62	0.34	SNP-R2-9	0.88	2	0.25	0.19
InDelR4-2	0.62	3	0.73	0.36	SNP-R2-10	0.62	2	0.77	0.36
InDelR5-1	0.54	3	0.88	0.37	SNP-R3-2	0.53	2	0.93	0.37
InDelR5-2	0.63	2	0.75	0.36	SNP-R3-3	0.87	2	0.27	0.20
InDelR5-3	0.65	2	0.70	0.35	SNP-R3-10	0.88	2	0.25	0.19
InDelR6-3	0.73	2	0.55	0.32	SNP-R4-1	0.75	2	0.50	0.30
InDelR7-1	0.67	2	0.67	0.35	SNP-R5-2	0.58	2	0.83	0.37
InDelR7-3	0.63	2	0.73	0.36	SNP-R6-5	0.69	3	0.48	0.34
InDelR8-1	0.67	2	0.67	0.35	SNP-R8-3	0.67	2	0.67	0.35
InDelR8-2	0.68	2	0.63	0.34

性状 Trait	标记名称 Marker	染色体 Chr	GLM		性状 Trait	标记名称 Marker	染色体 Chr	GLM
性状 Trait	标记名称 Marker	染色体 Chr	P值 P value	贡献率 Contribution rate/%	性状 Trait	标记名称 Marker	染色体 Chr	P值 P value	贡献率 Contribution rate/%
叶脉色	InDelR6-3	R06	0.000 2	15.60	肉质根表面光滑度	InDelR7-1	R07	0.001 1	11.78
Color of leaf veins	InDelR7-1	R07	0.000 3	15.20	Smoothness of the	InDelR8-2	R08	0.000 4	14.32
	InDelR8-2	R08	0.000 2	15.44	fleshy root surface	SNPR3-10	R03	0.004 2	8.86
	SNPR3-3	R03	0.006 6	7.96	根形Root form	SNPR4-1	R04	0.008 0	7.55
	SNPR4-1	R04	0.001 9	10.55		SNPR6-5	R06	0.008 0	7.55
	SNPR6-5	R06	0.001 9	10.55	肉色	InDelR6-3	R06	0.000 3	14.70
肉质根地上部皮色	InDelR4-2	R04	0.003 6	9.19	Color of flesh	InDelR7-1	R07	0.000 1	19.20
Color of aboveground	InDelR6-3	R06	0.000 9	12.34		InDelR8-2	R08	0.000 0	23.25
part of fleshy root	InDelR7-1	R07	0.000 8	12.60		SNPR2-10	R02	0.000 3	15.13
	InDelR8-2	R08	0.001 9	10.63		SNPR2-2	R02	0.000 2	15.27
	SNPR2-10	R02	0.002 9	9.66		SNPR3-10	R03	0.000 9	12.32
	SNPR2-2	R02	0.000 8	12.43		SNPR3-3	R03	0.008 9	7.33
	SNPR4-1	R04	0.000 1	17.94	肉质根地下部皮色	InDelR4-2	R04	0.002 0	10.43
	SNPR6-5	R06	0.000 1	17.94	Color of underground	InDelR5-3	R05	0.006 8	7.89
					part of fleshy root
叶缘Leaf margin	InDelR5-1	R05	0.003 5	9.30		InDelR7-1	R07	0.000 4	14.03
	SNPR1-15	R01	0.005 9	8.16		InDelR8-2	R08	0.000 8	12.59
肉质根侧根分布	InDelR6-3	R06	0.009 3	7.24		SNPR3-3	R03	0.000 9	12.14
Lateral root	InDelR7-3	R07	0.002 3	10.19		SNPR4-1	R04	0.000 0	19.26
distribution	InDelR9-1	R09	0.002 3	10.19		SNPR6-5	R06	0.000 0	19.26
	SNPR1-15	R01	0.006 6	7.93	叶形Leaf shape	InDelR5-2	R05	0.004 1	8.94
	SNPR2-10	R02	0.000 4	14.20		InDelR5-3	R05	0.006 9	7.84
	SNPR2-2	R02	0.009 1	7.29	叶色Leaf colour	InDelR7-3	R07	0.001 3	11.38
叶裂刻Leaf crack	InDelR2-1	R02	0.005 8	8.23		InDelR9-1	R09	0.001 3	11.38
					叶面毛刺Leaf seta	InDelR4-2	R04	0.006 8	7.87

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[8]	ZHONG Liping, WANG Jian, WU Xiaohua, WANG Ying, WU Xinyi, WANG Baogen, LU Zhongfu, WANG Huasen, LI Guojing. Genome wide association analysis of powdery mildew resistance of bottle gourd based on MAGIC population [J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2398-2407.
[9]	JIANG Ruiping, ZHAO Chenhui, LI Wenjie, AN Qiuju, LI Jialun, ZHOU Jiayu, LI Suiyan, LIAO Hai. Codon bias of IPI gene in leguminous plants [J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1114-1123.
[10]	ZHAO Yuhong, HE Wen, LI Gen, WANG Qiang, XIE Rui, WANG Yan, CHEN Qing, WANG Xiaorong. Fruit quality of Citrus maxima (Burm.) Merrill and its bud mutants varieties in Sichuan area [J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 995-1004.
[11]	LIU Tongjin, XU Mingjie, WANG Jinglei, LIU Liangfeng, CUI Qunxiang, BAO Chonglai, WANG Changyi. Genome-wide identification and expression analysis of ALMT gene family in radish [J]. Acta Agriculturae Zhejiangensis, 2022, 34(4): 746-755.
[12]	PEI Yun, XU Xiuhong, LU Jinbiao, CHEN Amin, ZHANG Wanping. Genetic diversity analysis of 151 cherry tomato resources in Guizhou Province [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 310-316.
[13]	WANG Xinle, BAI Junyan, LI Jingyun, LEI Ying, LI Gan, PANG Youzhi, DONG Zhihao, CHEN Yu, FAN Hongdeng, WANG Longwei, CHEN Mengke, ZENG Fanlin, AN Xiaokai, BAI Yongzhen. Association between polymorphism of vasoactive intestinal peptide type Ⅰ receptor gene and carcass traits in meat quails [J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2379-2385.
[14]	SHU Zaifa, LIU Yu, SHAO Jingna, ZHENG Shenghong, ZHOU Huijuan, JI Qingyong, HE Weizhong. Analysis of main quality components and selection of excellent resources of early-sprouting tea germplasm resources in southern Zhejiang, China [J]. Acta Agriculturae Zhejiangensis, 2022, 34(11): 2438-2450.
[15]	WANG Zhiqi, SUN Jian, LIANG Junchao, ZHAO Yunyan, YAN Tingxian, YAN Xiaowen, WEI Wenliang, LE Meiwang. Study on genetic diversity of sesame germplasm in Jiangxi Province based on molecular markers [J]. Acta Agriculturae Zhejiangensis, 2021, 33(9): 1565-1580.

序号 Numerical order	叶型 Leaf profile	叶形 Leaf shape	叶尖 Leaf opex	叶色 Leaf colour	叶脉色 Color of leaf veins	叶面毛刺 Leaf seta	肉质根地上部皮色 Color of aboveground part of fleshy root	叶缘 Leaf margin	叶裂刻 Leaf crack	肉质根侧根分布 Lateral root distribu- tion	肉质根表面光滑度 Smoothness of the fleshy root surface	根形 Root form	肉色 Color of flesh	肉质根地下部皮色 Color of underground part of fleshy root
1	2	6	3	3	5	2	7	2	2	1	1	9	5	5
2	2	6	2	4	5	1	7	2	2	0	2	7	6	5
3	2	6	3	4	5	1	7	2	2	0	2	13	6	5
4	2	6	4	3	1	1	7	2	2	0	2	11	5	1
5	2	6	4	3	4	1	7	2	3	0	1	1	1	5
6	1	7	1	3	4	2	7	1	1	0	1	3	1	5
7	2	6	1	4	4	1	7	3	3	3	1	13	1	5
8	1	5	3	4	2	2	4	0	1	1	2	7	5	4
9	2	6	4	4	4	2	7	2	2	0	1	2	1	5
10	2	6	2	3	4	0	7	2	2	1	1	5	6	5
11	2	6	2	4	6	1	9	3	3	2	2	7	7	6
12	1	6	2	4	3	3	1	2	2	0	1	10	1	1
13	2	6	1	3	2	0	1	3	3	1	1	9	1	1
14	2	6	3	4	3	3	3	2	2	2	2	1	1	1
15	2	6	2	4	3	2	1	3	2	3	1	13	1	1
16	2	6	2	3	3	1	4	1	3	0	2	2	3	2
17	2	6	4	4	3	1	5	2	2	1	1	13	3	2
18	2	6	4	4	2	1	3	2	2	2	1	1	1	1
19	1	3	4	4	2	0	1	1	0	3	1	3	1	1
20	1	6	2	3	5	2	9	3	3	3	1	3	1	1
21	2	6	2	3	6	1	9	2	3	3	1	1	1	6
22	2	4	2	2	2	1	1	1	2	2	2	3	1	1
23	1	6	4	4	4	2	7	2	0	2	1	5	1	5
24	2	6	1	4	2	3	3	3	3	2	1	3	1	1
25	2	6	3	3	3	1	2	2	2	2	2	7	6	4
26	2	6	4	4	3	1	4	2	2	1	1	9	2	1
27	1	4	3	1	2	1	1	2	1	1	1	9	1	1
28	2	6	3	4	2	3	1	2	2	2	2	7	1	1
29	2	6	2	4	3	2	1	2	2	2	1	13	1	1
30	2	6	1	4	2	2	1	3	3	2	1	1	1	1
31	1	4	2	4	4	0	7	2	1	3	1	13	1	4
32	2	6	4	4	2	1	2	2	2	3	1	13	1	1
33	2	6	1	4	2	0	1	3	3	3	1	1	1	1
34	1	6	4	4	2	1	4	2	0	1	1	9	1	1
35	2	6	4	4	3	2	1	2	2	2	1	3	1	1
36	2	6	4	4	2	1	1	2	3	1	1	13	1	1
37	2	6	4	4	2	1	1	2	3	2	1	13	1	1
38	2	6	2	4	2	1	1	3	3	2	1	7	1	1
39	1	6	2	3	4	2	7	2	0	2	1	7	1	4
40	2	6	2	3	2	0	1	3	3	1	1	11	1	1
41	2	6	4	4	3	3	1	1	2	2	1	5	1	1
42	2	6	2	4	3	3	1	2	2	3	1	7	1	1
43	2	6	2	4	2	1	1	3	3	3	1	13	1	1
44	2	6	4	4	3	1	1	2	2	2	1	3	1	1
45	1	4	1	3	3	1	1	3	1	2	2	13	1	1
46	2	6	2	4	2	2	5	3	3	3	1	13	1	1
47	2	6	2	4	2	2	1	3	3	2	1	3	1	1
48	2	6	2	3	2	1	4	2	2	1	1	13	3	1
49	2	6	2	4	3	1	5	3	3	1	1	13	2	1
50	2	6	2	4	2	2	1	3	3	3	1	13	1	1
51	2	6	4	3	3	2	2	1	2	2	1	2	1	1
52	2	6	4	4	3	3	1	2	2	1	2	13	1	1
53	1	4	4	4	3	2	1	2	0	2	2	13	1	1
54	1	6	2	4	2	1	1	3	0	2	1	13	1	1
55	1	6	2	4	2	2	2	3	0	1	1	13	1	1
56	2	6	2	3	4	2	7	1	2	1	1	5	7	1
57	1	6	3	3	2	2	1	3	1	1	1	11	1	1
58	1	6	2	4	2	2	1	2	1	1	1	11	1	1
59	1	4	2	3	4	2	7	3	0	1	1	10	1	5
60	1	4	4	3	4	3	7	2	0	1	1	5	4	1

序号 Numerical order	叶型 Leaf profile	叶形 Leaf shape	叶尖 Leaf opex	叶色 Leaf colour	叶脉色 Color of leaf veins	叶面毛刺 Leaf seta	肉质根地上部皮色 Color of aboveground part of fleshy root	叶缘 Leaf margin	叶裂刻 Leaf crack	肉质根侧根分布 Lateral root distribu- tion	肉质根表面光滑度 Smoothness of the fleshy root surface	根形 Root form	肉色 Color of flesh	肉质根地下部皮色 Color of underground part of fleshy root
1	2	6	3	3	5	2	7	2	2	1	1	9	5	5
2	2	6	2	4	5	1	7	2	2	0	2	7	6	5
3	2	6	3	4	5	1	7	2	2	0	2	13	6	5
4	2	6	4	3	1	1	7	2	2	0	2	11	5	1
5	2	6	4	3	4	1	7	2	3	0	1	1	1	5
6	1	7	1	3	4	2	7	1	1	0	1	3	1	5
7	2	6	1	4	4	1	7	3	3	3	1	13	1	5
8	1	5	3	4	2	2	4	0	1	1	2	7	5	4
9	2	6	4	4	4	2	7	2	2	0	1	2	1	5
10	2	6	2	3	4	0	7	2	2	1	1	5	6	5
11	2	6	2	4	6	1	9	3	3	2	2	7	7	6
12	1	6	2	4	3	3	1	2	2	0	1	10	1	1
13	2	6	1	3	2	0	1	3	3	1	1	9	1	1
14	2	6	3	4	3	3	3	2	2	2	2	1	1	1
15	2	6	2	4	3	2	1	3	2	3	1	13	1	1
16	2	6	2	3	3	1	4	1	3	0	2	2	3	2
17	2	6	4	4	3	1	5	2	2	1	1	13	3	2
18	2	6	4	4	2	1	3	2	2	2	1	1	1	1
19	1	3	4	4	2	0	1	1	0	3	1	3	1	1
20	1	6	2	3	5	2	9	3	3	3	1	3	1	1
21	2	6	2	3	6	1	9	2	3	3	1	1	1	6
22	2	4	2	2	2	1	1	1	2	2	2	3	1	1
23	1	6	4	4	4	2	7	2	0	2	1	5	1	5
24	2	6	1	4	2	3	3	3	3	2	1	3	1	1
25	2	6	3	3	3	1	2	2	2	2	2	7	6	4
26	2	6	4	4	3	1	4	2	2	1	1	9	2	1
27	1	4	3	1	2	1	1	2	1	1	1	9	1	1
28	2	6	3	4	2	3	1	2	2	2	2	7	1	1
29	2	6	2	4	3	2	1	2	2	2	1	13	1	1
30	2	6	1	4	2	2	1	3	3	2	1	1	1	1
31	1	4	2	4	4	0	7	2	1	3	1	13	1	4
32	2	6	4	4	2	1	2	2	2	3	1	13	1	1
33	2	6	1	4	2	0	1	3	3	3	1	1	1	1
34	1	6	4	4	2	1	4	2	0	1	1	9	1	1
35	2	6	4	4	3	2	1	2	2	2	1	3	1	1
36	2	6	4	4	2	1	1	2	3	1	1	13	1	1
37	2	6	4	4	2	1	1	2	3	2	1	13	1	1
38	2	6	2	4	2	1	1	3	3	2	1	7	1	1
39	1	6	2	3	4	2	7	2	0	2	1	7	1	4
40	2	6	2	3	2	0	1	3	3	1	1	11	1	1
41	2	6	4	4	3	3	1	1	2	2	1	5	1	1
42	2	6	2	4	3	3	1	2	2	3	1	7	1	1
43	2	6	2	4	2	1	1	3	3	3	1	13	1	1
44	2	6	4	4	3	1	1	2	2	2	1	3	1	1
45	1	4	1	3	3	1	1	3	1	2	2	13	1	1
46	2	6	2	4	2	2	5	3	3	3	1	13	1	1
47	2	6	2	4	2	2	1	3	3	2	1	3	1	1
48	2	6	2	3	2	1	4	2	2	1	1	13	3	1
49	2	6	2	4	3	1	5	3	3	1	1	13	2	1
50	2	6	2	4	2	2	1	3	3	3	1	13	1	1
51	2	6	4	3	3	2	2	1	2	2	1	2	1	1
52	2	6	4	4	3	3	1	2	2	1	2	13	1	1
53	1	4	4	4	3	2	1	2	0	2	2	13	1	1
54	1	6	2	4	2	1	1	3	0	2	1	13	1	1
55	1	6	2	4	2	2	2	3	0	1	1	13	1	1
56	2	6	2	3	4	2	7	1	2	1	1	5	7	1
57	1	6	3	3	2	2	1	3	1	1	1	11	1	1
58	1	6	2	4	2	2	1	2	1	1	1	11	1	1
59	1	4	2	3	4	2	7	3	0	1	1	10	1	5
60	1	4	4	3	4	3	7	2	0	1	1	5	4	1

序号 Numerical order	叶型 Leaf profile	叶形 Leaf shape	叶尖 Leaf opex	叶色 Leaf colour	叶脉色 Color of leaf veins	叶面毛刺 Leaf seta	肉质根地上部皮色 Color of aboveground part of fleshy root	叶缘 Leaf margin	叶裂刻 Leaf crack	肉质根侧根分布 Lateral root distribu- tion	肉质根表面光滑度 Smoothness of the fleshy root surface	根形 Root form	肉色 Color of flesh	肉质根地下部皮色 Color of underground part of fleshy root
1	2	6	3	3	5	2	7	2	2	1	1	9	5	5
2	2	6	2	4	5	1	7	2	2	0	2	7	6	5
3	2	6	3	4	5	1	7	2	2	0	2	13	6	5
4	2	6	4	3	1	1	7	2	2	0	2	11	5	1
5	2	6	4	3	4	1	7	2	3	0	1	1	1	5
6	1	7	1	3	4	2	7	1	1	0	1	3	1	5
7	2	6	1	4	4	1	7	3	3	3	1	13	1	5
8	1	5	3	4	2	2	4	0	1	1	2	7	5	4
9	2	6	4	4	4	2	7	2	2	0	1	2	1	5
10	2	6	2	3	4	0	7	2	2	1	1	5	6	5
11	2	6	2	4	6	1	9	3	3	2	2	7	7	6
12	1	6	2	4	3	3	1	2	2	0	1	10	1	1
13	2	6	1	3	2	0	1	3	3	1	1	9	1	1
14	2	6	3	4	3	3	3	2	2	2	2	1	1	1
15	2	6	2	4	3	2	1	3	2	3	1	13	1	1
16	2	6	2	3	3	1	4	1	3	0	2	2	3	2
17	2	6	4	4	3	1	5	2	2	1	1	13	3	2
18	2	6	4	4	2	1	3	2	2	2	1	1	1	1
19	1	3	4	4	2	0	1	1	0	3	1	3	1	1
20	1	6	2	3	5	2	9	3	3	3	1	3	1	1
21	2	6	2	3	6	1	9	2	3	3	1	1	1	6
22	2	4	2	2	2	1	1	1	2	2	2	3	1	1
23	1	6	4	4	4	2	7	2	0	2	1	5	1	5
24	2	6	1	4	2	3	3	3	3	2	1	3	1	1
25	2	6	3	3	3	1	2	2	2	2	2	7	6	4
26	2	6	4	4	3	1	4	2	2	1	1	9	2	1
27	1	4	3	1	2	1	1	2	1	1	1	9	1	1
28	2	6	3	4	2	3	1	2	2	2	2	7	1	1
29	2	6	2	4	3	2	1	2	2	2	1	13	1	1
30	2	6	1	4	2	2	1	3	3	2	1	1	1	1
31	1	4	2	4	4	0	7	2	1	3	1	13	1	4
32	2	6	4	4	2	1	2	2	2	3	1	13	1	1
33	2	6	1	4	2	0	1	3	3	3	1	1	1	1
34	1	6	4	4	2	1	4	2	0	1	1	9	1	1
35	2	6	4	4	3	2	1	2	2	2	1	3	1	1
36	2	6	4	4	2	1	1	2	3	1	1	13	1	1
37	2	6	4	4	2	1	1	2	3	2	1	13	1	1
38	2	6	2	4	2	1	1	3	3	2	1	7	1	1
39	1	6	2	3	4	2	7	2	0	2	1	7	1	4
40	2	6	2	3	2	0	1	3	3	1	1	11	1	1
41	2	6	4	4	3	3	1	1	2	2	1	5	1	1
42	2	6	2	4	3	3	1	2	2	3	1	7	1	1
43	2	6	2	4	2	1	1	3	3	3	1	13	1	1
44	2	6	4	4	3	1	1	2	2	2	1	3	1	1
45	1	4	1	3	3	1	1	3	1	2	2	13	1	1
46	2	6	2	4	2	2	5	3	3	3	1	13	1	1
47	2	6	2	4	2	2	1	3	3	2	1	3	1	1
48	2	6	2	3	2	1	4	2	2	1	1	13	3	1
49	2	6	2	4	3	1	5	3	3	1	1	13	2	1
50	2	6	2	4	2	2	1	3	3	3	1	13	1	1
51	2	6	4	3	3	2	2	1	2	2	1	2	1	1
52	2	6	4	4	3	3	1	2	2	1	2	13	1	1
53	1	4	4	4	3	2	1	2	0	2	2	13	1	1
54	1	6	2	4	2	1	1	3	0	2	1	13	1	1
55	1	6	2	4	2	2	2	3	0	1	1	13	1	1
56	2	6	2	3	4	2	7	1	2	1	1	5	7	1
57	1	6	3	3	2	2	1	3	1	1	1	11	1	1
58	1	6	2	4	2	2	1	2	1	1	1	11	1	1
59	1	4	2	3	4	2	7	3	0	1	1	10	1	5
60	1	4	4	3	4	3	7	2	0	1	1	5	4	1

序号 Numerical order	叶型 Leaf profile	叶形 Leaf shape	叶尖 Leaf opex	叶色 Leaf colour	叶脉色 Color of leaf veins	叶面毛刺 Leaf seta	肉质根地上部皮色 Color of aboveground part of fleshy root	叶缘 Leaf margin	叶裂刻 Leaf crack	肉质根侧根分布 Lateral root distribu- tion	肉质根表面光滑度 Smoothness of the fleshy root surface	根形 Root form	肉色 Color of flesh	肉质根地下部皮色 Color of underground part of fleshy root
1	2	6	3	3	5	2	7	2	2	1	1	9	5	5
2	2	6	2	4	5	1	7	2	2	0	2	7	6	5
3	2	6	3	4	5	1	7	2	2	0	2	13	6	5
4	2	6	4	3	1	1	7	2	2	0	2	11	5	1
5	2	6	4	3	4	1	7	2	3	0	1	1	1	5
6	1	7	1	3	4	2	7	1	1	0	1	3	1	5
7	2	6	1	4	4	1	7	3	3	3	1	13	1	5
8	1	5	3	4	2	2	4	0	1	1	2	7	5	4
9	2	6	4	4	4	2	7	2	2	0	1	2	1	5
10	2	6	2	3	4	0	7	2	2	1	1	5	6	5
11	2	6	2	4	6	1	9	3	3	2	2	7	7	6
12	1	6	2	4	3	3	1	2	2	0	1	10	1	1
13	2	6	1	3	2	0	1	3	3	1	1	9	1	1
14	2	6	3	4	3	3	3	2	2	2	2	1	1	1
15	2	6	2	4	3	2	1	3	2	3	1	13	1	1
16	2	6	2	3	3	1	4	1	3	0	2	2	3	2
17	2	6	4	4	3	1	5	2	2	1	1	13	3	2
18	2	6	4	4	2	1	3	2	2	2	1	1	1	1
19	1	3	4	4	2	0	1	1	0	3	1	3	1	1
20	1	6	2	3	5	2	9	3	3	3	1	3	1	1
21	2	6	2	3	6	1	9	2	3	3	1	1	1	6
22	2	4	2	2	2	1	1	1	2	2	2	3	1	1
23	1	6	4	4	4	2	7	2	0	2	1	5	1	5
24	2	6	1	4	2	3	3	3	3	2	1	3	1	1
25	2	6	3	3	3	1	2	2	2	2	2	7	6	4
26	2	6	4	4	3	1	4	2	2	1	1	9	2	1
27	1	4	3	1	2	1	1	2	1	1	1	9	1	1
28	2	6	3	4	2	3	1	2	2	2	2	7	1	1
29	2	6	2	4	3	2	1	2	2	2	1	13	1	1
30	2	6	1	4	2	2	1	3	3	2	1	1	1	1
31	1	4	2	4	4	0	7	2	1	3	1	13	1	4
32	2	6	4	4	2	1	2	2	2	3	1	13	1	1
33	2	6	1	4	2	0	1	3	3	3	1	1	1	1
34	1	6	4	4	2	1	4	2	0	1	1	9	1	1
35	2	6	4	4	3	2	1	2	2	2	1	3	1	1
36	2	6	4	4	2	1	1	2	3	1	1	13	1	1
37	2	6	4	4	2	1	1	2	3	2	1	13	1	1
38	2	6	2	4	2	1	1	3	3	2	1	7	1	1
39	1	6	2	3	4	2	7	2	0	2	1	7	1	4
40	2	6	2	3	2	0	1	3	3	1	1	11	1	1
41	2	6	4	4	3	3	1	1	2	2	1	5	1	1
42	2	6	2	4	3	3	1	2	2	3	1	7	1	1
43	2	6	2	4	2	1	1	3	3	3	1	13	1	1
44	2	6	4	4	3	1	1	2	2	2	1	3	1	1
45	1	4	1	3	3	1	1	3	1	2	2	13	1	1
46	2	6	2	4	2	2	5	3	3	3	1	13	1	1
47	2	6	2	4	2	2	1	3	3	2	1	3	1	1
48	2	6	2	3	2	1	4	2	2	1	1	13	3	1
49	2	6	2	4	3	1	5	3	3	1	1	13	2	1
50	2	6	2	4	2	2	1	3	3	3	1	13	1	1
51	2	6	4	3	3	2	2	1	2	2	1	2	1	1
52	2	6	4	4	3	3	1	2	2	1	2	13	1	1
53	1	4	4	4	3	2	1	2	0	2	2	13	1	1
54	1	6	2	4	2	1	1	3	0	2	1	13	1	1
55	1	6	2	4	2	2	2	3	0	1	1	13	1	1
56	2	6	2	3	4	2	7	1	2	1	1	5	7	1
57	1	6	3	3	2	2	1	3	1	1	1	11	1	1
58	1	6	2	4	2	2	1	2	1	1	1	11	1	1
59	1	4	2	3	4	2	7	3	0	1	1	10	1	5
60	1	4	4	3	4	3	7	2	0	1	1	5	4	1