The diversity of tea germplasm resources and genetic relationship of ‘Tieguanyin’-derived varieties in Anxi, Fujian, China

doi:10.3969/j.issn.1004-1524.20231037

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (7): 1591-1601.DOI: 10.3969/j.issn.1004-1524.20231037

• Horticultural Science • Previous Articles Next Articles

The diversity of tea germplasm resources and genetic relationship of ‘Tieguanyin’-derived varieties in Anxi, Fujian, China

ZHU Yanyu¹(), YU Wentao²^,^*(), GAO Shuilian³, LYU Shuiyuan², WANG Pan¹, JIN Wanmin³, GUI Wenjing¹, LIN Yi¹, YE Naixing¹^,^*()

1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Technology Centre of Fuzhou Customs District, Fuzhou 350001, China
3. Anxi Tea College, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2023-08-31 Online:2024-07-25 Published:2024-08-05

Abstract

Abstract:

In order to better protect, evaluate and utilize Anxi tea germplasm resources, the genetic diversity of Anxi tea germplasm resources and the genetic relationship of ‘Tieguanyin’-derived varieties were analyzed by single nucleotide polymorphism (SNP) molecular marker technique. Principal coordinate analysis, hierarchical clustering tree analysis, population structure analysis and genetic distance analysis were carried out.The DNA fingerprinting of ‘Tieguanyin’ and its derived varieties were also constructed, and their genetic similarity coefficients were calculated. The results showed that a total of 53 high polymorphic loci suitable for genotyping of Anxi tea germplasm resources were screened out. The average observed heterozygosity was 0.322, and the average expected heterozygosity was 0.281, indicating that Anxi tea plant germplasm resources had rich genetic diversity. The genetic distance between tea populations in the four townships of Anxi was 0.068-0.269. The tested samples could be divided into two groups. Group I mainly included tea plant germplasm resources from Xiping, Huqiu and Hushang, with frequent exchanges among germplasm resources. Group Ⅱ was mainly composed of wild-type landrace tea germplasm resources in Futian, and the tea plant germplasm resources in Futian were relatively concentrated and independent. DNA fingerprinting could accurately identify ‘Tieguanyin’ and its derived varieties. ‘Tieguanyin’-derived varieties had rich diversity, among which ‘Tieguanyin’ had a large genetic similarity coefficient with ‘Jinmudan’, ‘Zimeigui’ and ‘Xinxuan 211’. In summary, the diversity of tea plant germplasm resources in Anxi County was rich, ‘Jinmudan’, ‘Zimeigui’ and ‘Xinxuan 211’ had higher genetic similarity with ‘Tieguanyin’.

Key words: Anxi, ‘Tieguanyin’-derived variety, Camellia sinensis, germplasm resource, genetic diversity, single nucleotide polymorphism

CLC Number:

S571.1

ZHU Yanyu, YU Wentao, GAO Shuilian, LYU Shuiyuan, WANG Pan, JIN Wanmin, GUI Wenjing, LIN Yi, YE Naixing. The diversity of tea germplasm resources and genetic relationship of ‘Tieguanyin’-derived varieties in Anxi, Fujian, China[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1591-1601.

Figures/Tables 9

References 31

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主坐标 Principal coordinate	贡献率 Contribution rate	累计贡献率 Cumulative contribution rate
第1主坐标 The first principal coordinate	41.37	41.37
第2主坐标 The second principal coordinate	9.92	51.29
第3主坐标 The third principal coordinate	6.17	57.46

主坐标 Principal coordinate	贡献率 Contribution rate	累计贡献率 Cumulative contribution rate
第1主坐标 The first principal coordinate	41.37	41.37
第2主坐标 The second principal coordinate	9.92	51.29
第3主坐标 The third principal coordinate	6.17	57.46

地区 Area	西坪 Xiping	虎邱 Huqiu	湖上 Hushang	福田 Futian
虎邱Huqiu	0.081
湖上Hushang	0.072	0.068
福田Futian	0.269	0.101	0.231
其他Other	0.047	0.039	0.032	0.170

地区 Area	西坪 Xiping	虎邱 Huqiu	湖上 Hushang	福田 Futian
虎邱Huqiu	0.081
湖上Hushang	0.072	0.068
福田Futian	0.269	0.101	0.231
其他Other	0.047	0.039	0.032	0.170

品种 Variety	铁观音 Tieguan- yin	黄观音 Huang- guanyin	金观音 Jinguan- yin	紫玫瑰 Zimeigui	金牡丹 Jinmu- dan	春桃香 Chuntaoxiang	新选205 Xinxuan 205	新选206 Xinxuan 206	新选209 Xinxuan 209	新选211 Xinxuan 211	新选212 Xinxuan 212	金玫瑰 Jinmei- gui	紫牡丹 Zimudan	春兰 Chunlan	凤圆春 Fengyuan- chun	金茗早 Jinming- zao
黄观音	0.545
Huangguanyin
金观音	0.600	0.527
Jinguanyin
紫玫瑰	0.655	0.509	0.618
Zimeigui
金牡丹	0.655	0.491	0.655	0.782
Jinmudan
春桃香	0.509	0.436	0.582	0.509	0.545
Chuntaoxiang
新选205	0.618	0.491	0.800	0.636	0.727	0.564
Xinxuan 205
新选206	0.600	0.473	0.782	0.636	0.764	0.600	0.709
Xinxuan 206
新选209	0.600	0.473	0.655	0.745	0.673	0.545	0.600	0.709
Xinxuan 209
新选211	0.691	0.491	0.618	0.782	0.655	0.509	0.582	0.709	0.782
Xinxuan 211
新选212	0.636	0.491	0.691	0.673	0.655	0.545	0.673	0.691	0.709	0.709
Xinxuan 212
金玫瑰	0.491	0.400	0.582	0.673	0.600	0.636	0.655	0.618	0.655	0.618	0.564
Jinmeigui
紫牡丹	0.509	0.673	0.509	0.545	0.655	0.491	0.491	0.509	0.509	0.509	0.455	0.455
Zimudan
春兰	0.509	0.491	0.473	0.382	0.345	0.436	0.418	0.509	0.564	0.455	0.455	0.382	0.364
Chunlan
凤圆春	0.527	0.400	0.473	0.400	0.473	0.418	0.582	0.527	0.582	0.527	0.491	0.473	0.455	0.436
Fengyuanchun
金茗早	0.400	0.418	0.491	0.455	0.473	0.527	0.418	0.527	0.418	0.418	0.491	0.455	0.418	0.436	0.345
Jinmingzao
黄玫瑰	0.509	0.636	0.636	0.600	0.564	0.436	0.582	0.527	0.600	0.618	0.564	0.491	0.673	0.418	0.582	0.455
Huangmeigui

The diversity of tea germplasm resources and genetic relationship of ‘Tieguanyin’-derived varieties in Anxi, Fujian, China

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