Study on developmental changes of amino acid spectrum in Muscovy duck muscle

doi:10.3969/j.issn.1004-1524.20231019

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (9): 2010-2019.DOI: 10.3969/j.issn.1004-1524.20231019

• Animal Science • Previous Articles Next Articles

Study on developmental changes of amino acid spectrum in Muscovy duck muscle

PAN Zhihong¹^,²(), WEN Xueting²^,³, YANG Hua², LYU Wentao², ZHANG Junjie²^,³, XIAO Yingping²^,^*()

1. Key Laboratory of Genetic Breeding and Reproduction of Plateau and Mountain Animals, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China
2. State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. College of Animal Science and Technology, Zhejiang A&F University, Hangzhou 311300, China

Received:2023-08-24 Online:2024-09-25 Published:2024-09-30

Abstract

Abstract:

Samples of breast and leg muscles from the same batch of female Muscovy ducks at 14, 28, 42, 70, and 660 days of age were collected post-slaughter, and the changes in moisture, protein, and amino acid content were determined, to clarify the developmental changes in amino acid profiles of Muscovy duck breast and leg muscles. Results indicated that both breast and leg muscle weights increased significantly (P<0.05) with age, while the protein content increased overall yet the moisture content gradually decreased during the entire developmental process. The total amount of amino acids and essential amino acids in breast muscles increased with age, showing no significant difference between 70 and 660 days but significantly higher levels compared to 14, 28, and 42 days. Lysine and cysteine levels were significantly higher at 70 days, while arginine, tyrosine, valine, phenylalanine, isoleucine, and proline levels were significantly higher at 660 days. In leg muscles, total amino acid content, essential amino acids, and umami amino acids peaked at 70 days, with significantly higher levels of alanine, leucine, and lysine compared to other ages. Cysteine, threonine, and tyrosine levels were significantly higher at 660 days. Orthogonal partial least squares discriminant analysis revealed differences in amino acid profiles between breast and leg muscles of Muscovy ducks at different ages, with lysine and cysteine identified as the most important amino acids contributing to metabolic profile differences from 14 to 660 days. In conclusion, amino acid content in Muscovy duck muscles undergoes significant changes with age, with notable peaks in total amino acids, essential amino acids, and umami amino acids in leg muscles at 70 days, and in total amino acids and essential amino acids in breast muscles at 660 days.

Key words: Muscovy duck, days of age, muscle, amino acid, principal component analysis

CLC Number:

S834

PAN Zhihong, WEN Xueting, YANG Hua, LYU Wentao, ZHANG Junjie, XIAO Yingping. Study on developmental changes of amino acid spectrum in Muscovy duck muscle[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2010-2019.

Figures/Tables 10

References 32

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指标 Index	各生育阶段的营养水平 Nutrient level at different growth stages
指标 Index	0~21 d	22~180 d	181~660 d
代谢能	12.22	12.01	11.56
Metabolizable energy/ (MJ·kg^-1)
粗蛋白质含量	20.08	19.42	18.15
Crude protein content/%
赖氨酸含量	0.91	0.90	1.12
Lysine content/%
蛋氨酸含量	0.48	0.46	0.52
Methionine content/%
钙含量Calcium content/%	0.85	0.85	3.81
总磷含量	0.48	0.48	0.63
Phosphorus content/%

指标 Index	各生育阶段的营养水平 Nutrient level at different growth stages
指标 Index	0~21 d	22~180 d	181~660 d
代谢能	12.22	12.01	11.56
Metabolizable energy/ (MJ·kg^-1)
粗蛋白质含量	20.08	19.42	18.15
Crude protein content/%
赖氨酸含量	0.91	0.90	1.12
Lysine content/%
蛋氨酸含量	0.48	0.46	0.52
Methionine content/%
钙含量Calcium content/%	0.85	0.85	3.81
总磷含量	0.48	0.48	0.63
Phosphorus content/%

日龄 Age in days/d	体重 Body weight/g	胸肌重 Breast muscle weight/g	腿肌重 Leg muscle weight/g	胸肌率 Percentage of breast muscle yield/%	腿肌率 Percentage of leg muscle yield/%
14	287.30±6.37 e	3.66±0.36 e	29.46±3.56 e	1.27±0.01 d	10.20±0.01 a
28	1 233.75±58.36 d	29.33±2.68 d	59.80±3.92 d	2.40±0.01 c	4.68±0.01 b
42	1 802.50±63.72 c	79.03±2.34 c	100.15±7.71 c	4.39±0.01 b	5.56±0.01 b
70	2 946.25±62.13 b	303.90±12.69 b	179.70±10.90 b	10.32±0.01 a	6.61±0.01 b
660	3 466.25±163.50 a	345.09±15.69 a	211.61±12.34 a	9.96±0.01 a	6.13±0.01 b

日龄 Age in days/d	体重 Body weight/g	胸肌重 Breast muscle weight/g	腿肌重 Leg muscle weight/g	胸肌率 Percentage of breast muscle yield/%	腿肌率 Percentage of leg muscle yield/%
14	287.30±6.37 e	3.66±0.36 e	29.46±3.56 e	1.27±0.01 d	10.20±0.01 a
28	1 233.75±58.36 d	29.33±2.68 d	59.80±3.92 d	2.40±0.01 c	4.68±0.01 b
42	1 802.50±63.72 c	79.03±2.34 c	100.15±7.71 c	4.39±0.01 b	5.56±0.01 b
70	2 946.25±62.13 b	303.90±12.69 b	179.70±10.90 b	10.32±0.01 a	6.61±0.01 b
660	3 466.25±163.50 a	345.09±15.69 a	211.61±12.34 a	9.96±0.01 a	6.13±0.01 b

项目 Item	不同日龄(d)番鸭中的含量Content in Muscovy ducks at different ages (d)
项目 Item	14	28	42	70	660
天冬氨酸Aspartic acid(Asp)	0.84±0.03 c	1.34±0.04 b	1.78±0.03 a	1.80±0.08 a	1.88±0.08 a
谷氨酸Glutamic acid(Glu)	1.28±0.05 e	1.97±0.07 d	3.55±0.08 a	2.94±0.12 b	2.67±0.06 c
丝氨酸Serine(Ser)	0.39±0.02 b	0.71±0.03 a	0.75±0.02 a	0.73±0.04 a	0.80±0.03 a
组氨酸Histidine(His)	0.27±0.01 c	1.19±0.03 a	0.10±0.00 d	0.19±0.04 cd	0.78±0.07 b
甘氨酸Glycine(Gly)	0.59±0.01 c	0.69±0.05 c	1.30±0.03 a	0.91±0.04 b	0.84±0.04 b
苏氨酸Threonine(Thr)	0.60±0.02 bc	1.22±0.05 a	0.54±0.03 c	0.74±0.06 b	1.30±0.07 a
精氨酸Arginine(Arg)	0.59±0.02 e	0.76±0.03 d	0.89±0.01 c	1.00±0.05 b	1.20±0.03 a
丙氨酸Alanine(Ala)	0.59±0.01 c	0.69±0.03 c	1.12±0.02 b	1.42±0.08 a	1.37±0.04 a
酪氨酸Tyrosine(Tyr)	0.26±0.01 d	0.35±0.01 bc	0.30±0.01 cd	0.38±0.03 b	0.60±0.02 a
胱氨酸Cystine(Cys)	0.68±0.02 d	0.67±0.03 d	0.88±0.02 c	1.69±0.05 a	1.49±0.04 b
缬氨酸Valine(Val)	0.23±0.01 c	0.29±0.01 b	0.20±0.01 c	0.34±0.03 b	0.52±0.02 a
苯丙氨酸Phenylalanine(Phe)	0.36±0.01 d	0.50±0.02 c	0.37±0.01 d	0.66±0.03 b	0.79±0.03 a
甲硫氨酸Methionine(Met)	0.54±0.02 d	0.76±0.02 c	1.25±0.02 a	1.02±0.04 b	1.27±0.05 a
异亮氨酸Isoleucine(Ile)	0.68±0.03 e	0.94±0.04 d	1.08±0.02 c	1.36±0.06 b	1.62±0.05 a
亮氨酸Leucine(Leu)	0.42±0.03 d	1.04±0.03 a	0.51±0.01 c	0.84±0.02 b	1.01±0.05 a
赖氨酸Lysine(Lys)	0.27±0.10 d	1.52±0.06 b	0.85±0.10 c	2.27±0.33 a	1.41±0.16 b
脯氨酸Proline(Pro)	0.47±0.03 c	0.39±0.01 d	0.64±0.03 b	0.60±0.03 b	0.74±0.03 a
合计Total	9.08±0.30 c	15.03±0.40 b	16.11±0.30 b	18.86±1.00 a	20.29±0.67 a

Study on developmental changes of amino acid spectrum in Muscovy duck muscle

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项目 Item	不同日龄(d)番鸭中的含量Content in Muscovy ducks at different ages (d)
项目 Item	14	28	42	70	660
天冬氨酸Aspartic acid(Asp)	0.95±0.14 d	1.29±0.05 c	1.89±0.13 b	2.25±0.08 a	2.52±0.09 a
谷氨酸Glutamic acid(Glu)	1.51±0.24 c	1.90±0.04 c	3.52±0.34 b	4.36±0.16 a	4.25±0.12 a
丝氨酸Serine(Ser)	0.47±0.06 c	0.64±0.02 b	0.80±0.05 a	0.91±0.03 a	0.55±0.03 bc
组氨酸Histidine(His)	0.54±0.21 b	0.47±0.08 b	0.82±0.68 ab	0.30±0.03 c	1.10±0.04 a
甘氨酸Glycine(Gly)	0.13±0.09 c	0.21±0.13 c	0.84±0.27 ab	1.22±0.06 a	0.66±0.05 b
苏氨酸Threonine(Thr)	0.39±0.01 d	0.50±0.09 cd	0.70±0.08 bc	0.86±0.04 b	2.30±0.11 a
精氨酸Arginine(Arg)	0.61±0.13 c	0.74±0.05 bc	0.91±0.05 ab	1.12±0.03 a	0.39±0.06 d
丙氨酸Alanine(Ala)	0.49±0.10 c	0.59±0.05 c	1.19±0.09 b	1.41±0.04 a	1.18±0.06 b
酪氨酸Tyrosine(Tyr)	0.25±0.05 d	0.32±0.02 cd	0.38±0.04 bc	0.44±0.02 b	0.68±0.02 a
胱氨酸Cystine(Cys)	0.48±0.07 c	0.63±0.07 c	0.89±0.15 b	0.38±0.05 c	1.29±0.04 a
缬氨酸Valine(Val)	0.21±0.04 a	0.23±0.01 a	0.31±0.08 a	0.23±0.09 a	0.23±0.06 a
苯丙氨酸Phenylalanine(Phe)	0.33±0.07 b	0.46±0.05 ab	0.45±0.04 b	0.59±0.03 a	0.08±0.03 c
甲硫氨酸Methionine(Met)	0.58±0.09 b	0.74±0.01 b	1.38±0.09 a	1.22±0.03 a	0.77±0.06 b
异亮氨酸Isoleucine(Ile)	0.62±0.13 b	0.76±0.07 b	1.29±0.08 a	1.39±0.04 a	1.24±0.06 a
亮氨酸Leucine(Leu)	0.28±0.04 c	0.45±0.11 bc	0.76±0.18 b	1.37±0.04 a	0.74±0.06 b
赖氨酸Lysine(Lys)	0.15±0.03 c	0.40±0.25 c	1.06±0.23 b	1.87±0.06 a	0.95±0.06 b
脯氨酸Proline(Pro)	0.18±0.03 b	0.25±0.05 b	0.55±0.10 a	0.67±0.06 a	0.55±0.02 a
合计Total	8.16±1.52 b	10.57±1.13 b	17.74±0.98 a	20.58±0.79 a	19.47±0.86 a

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