Abstract: According to the conserved gene sequences of Duck plague virus(DPV) in GenBank, one pair of specific primer was designed. A rapid diagnostic technique of PCR was established by optimizing the reaction condition. The DNA segments amplified were 1 293 bp. The experiments had proved that PCR possessed a high specificity. And the sensitivity test results indicated that PCR was more sensitive, which could detect DHV with only 15 pg·mL^-1 DNA. Detection of field samples from viral diseased ducks also confirmed that the PCR method could identify the infection of DPV, which was specific, rapid and sensitive．

Key words: Duck hepatitis virus, PCR, detection