Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects

doi:10.3969/j.issn.1004-1524.20250337

Acta Agriculturae Zhejiangensis ›› 2026, Vol. 38 ›› Issue (4): 669-679.DOI: 10.3969/j.issn.1004-1524.20250337

• Horticultural Science • Previous Articles Next Articles

Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects

MAO Mingjuan¹^,²(), PU Qiniu¹^,², HOU Lijuan¹^,²^,^*(), MA Lin¹, JIANG Ning¹, LIN Jinsheng¹, QU Shaoxuan¹, LI Huiping¹, XU Ping¹, LIU Di²

¹ Jiangsu Key Laboratory of Horticultural Crops Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
² College of Agriculture, Yanbian University, Yanji 133002, Jilin, China

Received:2025-04-25 Online:2026-04-25 Published:2026-05-08
Contact: HOU Lijuan

Abstract

Abstract:

Exogenous sodium acetate (NaAc) can promote indole-3-acetic acid (IAA) synthesis during the primordium stage of Volvariella volvacea, thereby promoting primordium differentiation and increasing yield, but its specific mechanism of action has not been elucidated. This study aimed to analyze the ability of NaAc to induce IAA synthesis. Using a submerged culture method, a three-factor experiment was designed with five pH value gradients(6, 7, 8, 9, 10), four anions (Ac^-, HCO₃^-, C₅H₇O₅COO^-, H₂PO₄^-), and four cations (Na⁺, Mg²⁺, Mn²⁺, K⁺) to investigate the effects of adding NaAc on mycelial biomass and IAA synthesis, providing a theoretical basis for subsequently revealing the mechanism by which NaAc regulates IAA synthesis. The results showed that under pH value 8 conditions, mycelial biomass, aldehyde dehydrogenase (ALDH) activity, and the contents of IAA, indole-3-acetaldehyde (IAAld), indole-3-pyruvic acid (IPyA), tryptamine (TAM) and indole-3-acetaldoxime (IAOx) were significantly (p<0.05) increased by 37.98%, 58.39%, 61.29%, 58.33%, 31.25%, 29.23% and 64.71%, respectively. Among the anions, acetate ions (Ac^-) significantly increased biomass, IAA content, and ALDH activity by 6.28%, 22.58%, and 21.01%, respectively, the contents of IPyA, TAM, and IAOx were also significantly higher than the control. Among the cations, Na⁺ significantly increased biomass, IAA content, and ALDH activity by 6.28%, 22.58% and 20.97%, respectively, and the contents of IPyA, TAM, and IAOx were also significantly higher than the control. This indicated that Na⁺ was superior to Mg²⁺, Mn²⁺, and K⁺ in promoting IAA synthesis. Comprehensive analysis showed that during the vegetative growth stage of V. volvacea, adding NaAc could maintain the culture environment pH value within an alkaline range suitable for growth. pH value, as the main regulatory factor after NaAc dissociation, together with Ac^- and Na⁺, influenced biomass accumulation and IAA synthesis during the growth stage of V. volvacea.

Key words: Volvariella volvacea, pH value, ionic effect, IAA synthesis, biomass

CLC Number:

S646.13

MAO Mingjuan, PU Qiniu, HOU Lijuan, MA Lin, JIANG Ning, LIN Jinsheng, QU Shaoxuan, LI Huiping, XU Ping, LIU Di. Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects[J]. Acta Agriculturae Zhejiangensis, 2026, 38(4): 669-679.

Figures/Tables 6

Fig.1 Effect of initial pH value on mycelial growth and biomass of Volvariella volvacea Different lowercase letters above the bars indicate significant differences at the p<0.05 level. The same as below.

Fig.2 Effects of different initial pH values on the key products of IAA synthesis and the activity of aldehyde dehydrogenase in Volvariella volvacea IAA, Indole-3-acetic acid; TAM, Tryptamine; IPyA, Indole-3-pyruvate; IAAld, Indole-3-acetaldehyde; IAOx, Indole-3-acetaldehyde oxime; ALDH, Aldehyde dehydrogenase. The same as below.

Fig.3 Effects of different anions on mycelial growth and biomass of Volvariella volvacea

Fig.4 Effects of different buffers on the key products of IAA synthesis pathway and the activity of aldehyde dehydrogenase in Volvariella volvacea

Fig.5 Effects of different cations on mycelial growth and biomass of Volvariella volvacea

Fig.6 Effects of different cations on the contents of key products of the IAA synthesis pathway and the activity of aldehyde dehydrogenase in Volvariella volvacea

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Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects

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