Abstract:

Lytic polysaccharide monooxygenase (LPMO) is an oxidase whose biochemical characteristics, role in lignocellulosic degradation, protein engineering and optimization, and applications in industrial biotechnology have attracted widespread attention. Due to its complex structure and chemical composition, lignocellulose is difficult to degrade efficiently under natural conditions, and its effective degradation and transformation have become an important challenge. LPMO cleaves the crystalline surfaces of polysaccharides through oxidative reactions and synergizes with glycosidases to efficiently degrade lignocellulose. This function enables LPMO to play an important role in facilitating the degradation of naturally recalcitrant materials such as straw. The adoption of heterologous expression to produce LPMO in other hosts is an effective strategy to boost LPMO production and promote its industrialization. At present, numerous successful cases of LPMO genetic engineering have been reported, where its stability, activity, substrate specificity, and catalytic efficiency have been enhanced through various strategies. LPMO has found broad applications in industrial biotechnology, including disease-resistant plant, the food industry, and animal husbandry, demonstrating considerable potential. Future research may focus on further elucidating the catalytic degradation mechanism of LPMO, expanding studies on efficient heterologous expression and protein engineering, and advancing the industrial utilization of LPMO.

Key words: lytic polysaccharide monooxygenase, lignocellulose, protein engineering, biomass