浙江农业学报 ›› 2022, Vol. 34 ›› Issue (10): 2199-2208.DOI: 10.3969/j.issn.1004-1524.2022.10.14
杨海涛(), 张婧, 马红艳, 杨滟, 丁东霞, 吕剑, 颉建明(
)
收稿日期:
2021-03-17
出版日期:
2022-10-25
发布日期:
2022-10-26
通讯作者:
颉建明
作者简介:
*颉建明,E-mail: xiejianming@gsau.edu.cn基金资助:
YANG Haitao(), ZHANG Jing, MA Hongyan, YANG Yan, DING Dongxia, LYU Jian, XIE Jianming(
)
Received:
2021-03-17
Online:
2022-10-25
Published:
2022-10-26
Contact:
XIE Jianming
摘要:
采用水培方式,以亚硒酸钠和DL-硒代蛋氨酸为硒源,研究不同硒源对小白菜品质、产量以及营养元素含量的影响以期筛选出适宜的施用浓度和硒源。结果表明,亚硒酸钠和DL-硒代蛋氨酸各处理均可不同程度地提高小白菜叶片可溶性糖、游离氨基酸、可溶性蛋白含量,降低小白菜硝酸盐和粗纤维含量,DL-硒代蛋氨酸各处理还可提高小白菜叶片维生素C含量。施入外源硒后,小白菜植株硒含量随施硒浓度的上升而增加。硝酸盐与可溶性糖、硝酸盐与可溶性蛋白、粗纤维与维生素C呈极显著负相关。主成分分析结果表明,各处理下小白菜品质综合排名依次为 T2>T1>S2>T3>S1>S3>CK>T4>S4。T2处理小白菜的可溶性蛋白和游离氨基酸含量分别提高了2.82百分点和107.14%,硝酸盐含量降低了51.21%,地上部硒含量为0.843 mg·kg-1。综合分析,以亚硒酸钠为硒源,施用Se浓度为0.5 mg·L-1时效果最佳。
中图分类号:
杨海涛, 张婧, 马红艳, 杨滟, 丁东霞, 吕剑, 颉建明. 不同外源硒对小白菜品质及营养元素含量的影响[J]. 浙江农业学报, 2022, 34(10): 2199-2208.
YANG Haitao, ZHANG Jing, MA Hongyan, YANG Yan, DING Dongxia, LYU Jian, XIE Jianming. Effects of different selenium sources on quality and nutrient absorption of pakchoi[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2199-2208.
图1 不同外源硒对小白菜可溶性糖(A)、VC(B)、游离氨基酸(C)和可溶性蛋白(D)含量的影响不同处理间没有相同小写字母表示差异显著(P<0.05),下同。
Fig.1 Effects of different selenium sources on soluble sugar(A), vitamin C(B), free amino acids(C) and soluble protein(D) of pakchoi The bars of different treatments without the same lowercase letters showed the significant difference (P<0.05). The same as below.
处理 Treatment | 地上部鲜重 Fresh weight of shoots/(g·pot-1) | 处理 Treatment | 地上部鲜重 Fresh weight of shoots/(g·pot-1) |
---|---|---|---|
CK | 89.93±8.18 b | CK | 89.93±8.18 a |
T1 | 98.83±4.97 ab | S1 | 98.70±8.52 a |
T2 | 113.87±18.31 a | S2 | 107.80±10.15 a |
T3 | 92.00±8.74 b | S3 | 95.73±11.38 a |
T4 | 86.17±5.65 b | S4 | 88.27±12.87 a |
表1 不同外源硒对小白菜产量的影响
Table 1 Effects of different selenium sources on the yield of pakchoi
处理 Treatment | 地上部鲜重 Fresh weight of shoots/(g·pot-1) | 处理 Treatment | 地上部鲜重 Fresh weight of shoots/(g·pot-1) |
---|---|---|---|
CK | 89.93±8.18 b | CK | 89.93±8.18 a |
T1 | 98.83±4.97 ab | S1 | 98.70±8.52 a |
T2 | 113.87±18.31 a | S2 | 107.80±10.15 a |
T3 | 92.00±8.74 b | S3 | 95.73±11.38 a |
T4 | 86.17±5.65 b | S4 | 88.27±12.87 a |
部位 Part | 处理 Treatment | N含量 N content/ (g·kg-1) | P含量 P content/ (g·kg-1) | K含量 K content/ (g·kg-1) | Ca含量 Ca content/ (mg·kg-1) | Mg含量 Mg content/ (mg·kg-1) |
---|---|---|---|---|---|---|
地上部 | CK | 20.54±0.30 a | 5.67±0.85 b | 21.56±0.12 a | 4.07±0.13 d | 3.22±0.28 cd |
Shoots | T1 | 19.94±0.38 ab | 6.93±0.28 a | 20.27±0.19 b | 4.19±0.04 cd | 3.69±0.02 a |
T2 | 19.46±0.08 b | 6.30±0.18 ab | 20.00±0.41 b | 4.66±0.09 a | 3.55±0.06 ab | |
T3 | 19.94±0.85 ab | 5.99±0.31 b | 20.28±0.10 b | 4.23±0.02 bc | 3.53±0.01 ab | |
T4 | 20.15±0.41 ab | 6.20±0.28 ab | 20.24±0.26 b | 4.34±0.02 b | 3.41±0.03 bc | |
地下部 | CK | 14.72±0.11 b | 15.98±0.85 a | 12.20±0.41 b | 9.60±0.10 c | 4.12±0.07 a |
Roots | T1 | 12.48±0.21 c | 13.52±0.28 b | 8.60±1.13 d | 11.37±0.05 a | 3.78±0.02 b |
T2 | 13.02±0.50 c | 14.42±0.18 b | 10.13±0.73 c | 10.74±0.24 b | 3.88±0.01 b | |
T3 | 14.61±0.09 b | 13.60±0.31 b | 12.05±0.32 b | 9.25±0.12 d | 3.82±0.02 b | |
T4 | 16.65±0.56 a | 14.09±0.28 b | 13.77±0.76 a | 6.56±0.20 e | 3.76±0.13 b |
表2 外源亚硒酸钠对小白菜N、P、K、Ca、Mg元素含量的影响
Table 2 Effects of different selenium sources on the contents of N, P, K, Ca, Mg in pakchoi
部位 Part | 处理 Treatment | N含量 N content/ (g·kg-1) | P含量 P content/ (g·kg-1) | K含量 K content/ (g·kg-1) | Ca含量 Ca content/ (mg·kg-1) | Mg含量 Mg content/ (mg·kg-1) |
---|---|---|---|---|---|---|
地上部 | CK | 20.54±0.30 a | 5.67±0.85 b | 21.56±0.12 a | 4.07±0.13 d | 3.22±0.28 cd |
Shoots | T1 | 19.94±0.38 ab | 6.93±0.28 a | 20.27±0.19 b | 4.19±0.04 cd | 3.69±0.02 a |
T2 | 19.46±0.08 b | 6.30±0.18 ab | 20.00±0.41 b | 4.66±0.09 a | 3.55±0.06 ab | |
T3 | 19.94±0.85 ab | 5.99±0.31 b | 20.28±0.10 b | 4.23±0.02 bc | 3.53±0.01 ab | |
T4 | 20.15±0.41 ab | 6.20±0.28 ab | 20.24±0.26 b | 4.34±0.02 b | 3.41±0.03 bc | |
地下部 | CK | 14.72±0.11 b | 15.98±0.85 a | 12.20±0.41 b | 9.60±0.10 c | 4.12±0.07 a |
Roots | T1 | 12.48±0.21 c | 13.52±0.28 b | 8.60±1.13 d | 11.37±0.05 a | 3.78±0.02 b |
T2 | 13.02±0.50 c | 14.42±0.18 b | 10.13±0.73 c | 10.74±0.24 b | 3.88±0.01 b | |
T3 | 14.61±0.09 b | 13.60±0.31 b | 12.05±0.32 b | 9.25±0.12 d | 3.82±0.02 b | |
T4 | 16.65±0.56 a | 14.09±0.28 b | 13.77±0.76 a | 6.56±0.20 e | 3.76±0.13 b |
部位 Part | 处理 Treatment | N含量 N content/ (g·kg-1) | P含量 P content/ (g·kg-1) | K含量 K content/ (g·kg-1) | Ca含量 Ca content/ (mg·kg-1) | Mg含量 Mg content/ (mg·kg-1) |
---|---|---|---|---|---|---|
地上部 | CK | 20.54±0.30 a | 5.67±0.85 ab | 21.56±0.12 a | 4.07±0.13 b | 3.22±0.28 a |
Shoots | S1 | 19.39±0.17 b | 3.38±0.18 c | 17.17±0.15 d | 4.37±0.05 a | 3.42±0.09 a |
S2 | 18.06±0.46 c | 4.74±0.08 b | 15.61±0.28 e | 4.13±0.06 b | 3.41±0.07 a | |
S3 | 19.98±0.66 ab | 5.44±0.36 ab | 17.95±0.41 c | 4.29±0.03 a | 3.43±0.07 a | |
S4 | 20.74±0.24 a | 6.19±0.68 a | 18.89±0.41 b | 4.28±0.02 a | 3.45±0.07 a | |
地下部 | CK | 14.72±0.11 b | 15.98±0.41 a | 12.20±0.05 a | 9.60±0.10 a | 4.12±0.07 bc |
Roots | S1 | 15.67±0.23 a | 14.10±0.13 b | 11.85±0.05 b | 9.26±0.23 a | 4.16±0.01 bc |
S2 | 16.16±0.15 a | 13.37±1.13 b | 11.68±0.15 c | 9.51±0.22 a | 4.59±0.05 a | |
S3 | 15.69±0.29 a | 11.58±0.28 c | 11.22±0.08 d | 8.67±0.02 b | 4.24±0.16 b | |
S4 | 16.10±0.62 a | 11.13±0.32 c | 12.11±0.06 a | 8.43±0.40 b | 3.96±0.23 c |
表3 外源DL-硒代蛋氨酸对小白菜N、P、K、Ca、Mg元素含量的影响
Table 3 Effects of different selenium sources on the contents of N, P, K, Ca, Mg in pakchoi
部位 Part | 处理 Treatment | N含量 N content/ (g·kg-1) | P含量 P content/ (g·kg-1) | K含量 K content/ (g·kg-1) | Ca含量 Ca content/ (mg·kg-1) | Mg含量 Mg content/ (mg·kg-1) |
---|---|---|---|---|---|---|
地上部 | CK | 20.54±0.30 a | 5.67±0.85 ab | 21.56±0.12 a | 4.07±0.13 b | 3.22±0.28 a |
Shoots | S1 | 19.39±0.17 b | 3.38±0.18 c | 17.17±0.15 d | 4.37±0.05 a | 3.42±0.09 a |
S2 | 18.06±0.46 c | 4.74±0.08 b | 15.61±0.28 e | 4.13±0.06 b | 3.41±0.07 a | |
S3 | 19.98±0.66 ab | 5.44±0.36 ab | 17.95±0.41 c | 4.29±0.03 a | 3.43±0.07 a | |
S4 | 20.74±0.24 a | 6.19±0.68 a | 18.89±0.41 b | 4.28±0.02 a | 3.45±0.07 a | |
地下部 | CK | 14.72±0.11 b | 15.98±0.41 a | 12.20±0.05 a | 9.60±0.10 a | 4.12±0.07 bc |
Roots | S1 | 15.67±0.23 a | 14.10±0.13 b | 11.85±0.05 b | 9.26±0.23 a | 4.16±0.01 bc |
S2 | 16.16±0.15 a | 13.37±1.13 b | 11.68±0.15 c | 9.51±0.22 a | 4.59±0.05 a | |
S3 | 15.69±0.29 a | 11.58±0.28 c | 11.22±0.08 d | 8.67±0.02 b | 4.24±0.16 b | |
S4 | 16.10±0.62 a | 11.13±0.32 c | 12.11±0.06 a | 8.43±0.40 b | 3.96±0.23 c |
处理 Treatment | 主成分得分Scores of principal component | 综合得分(F) Composite score | 排名 Ranking | |||
---|---|---|---|---|---|---|
PC1(F1) | PC2(F2) | PC3(F3) | PC4(F4) | |||
CK | -0.933 76 | 1.660 68 | -1.015 03 | -1.457 23 | -0.33 | 7 |
T1 | 0.696 95 | 1.183 01 | -0.817 17 | 1.665 46 | 0.67 | 2 |
T2 | 0.198 58 | 0.880 04 | 2.330 22 | 0.080 08 | 0.81 | 1 |
T3 | -0.396 07 | -0.015 24 | 0.361 39 | 0.648 74 | 0.04 | 4 |
T4 | -0.506 24 | -0.693 72 | -0.578 07 | 0.727 23 | -0.37 | 8 |
S1 | 0.769 27 | -0.618 37 | 0.201 51 | -1.407 93 | -0.08 | 5 |
S2 | 1.863 08 | -0.658 13 | -0.595 87 | -0.304 34 | 0.31 | 3 |
S3 | -0.256 06 | -0.508 35 | 0.107 56 | -0.163 81 | -0.23 | 6 |
S4 | -1.435 74 | -1.229 94 | 0.005 46 | 0.211 82 | -0.81 | 9 |
表4 各主成分综合得分及排名
Table 4 Comprehensive scores of main component and ranking
处理 Treatment | 主成分得分Scores of principal component | 综合得分(F) Composite score | 排名 Ranking | |||
---|---|---|---|---|---|---|
PC1(F1) | PC2(F2) | PC3(F3) | PC4(F4) | |||
CK | -0.933 76 | 1.660 68 | -1.015 03 | -1.457 23 | -0.33 | 7 |
T1 | 0.696 95 | 1.183 01 | -0.817 17 | 1.665 46 | 0.67 | 2 |
T2 | 0.198 58 | 0.880 04 | 2.330 22 | 0.080 08 | 0.81 | 1 |
T3 | -0.396 07 | -0.015 24 | 0.361 39 | 0.648 74 | 0.04 | 4 |
T4 | -0.506 24 | -0.693 72 | -0.578 07 | 0.727 23 | -0.37 | 8 |
S1 | 0.769 27 | -0.618 37 | 0.201 51 | -1.407 93 | -0.08 | 5 |
S2 | 1.863 08 | -0.658 13 | -0.595 87 | -0.304 34 | 0.31 | 3 |
S3 | -0.256 06 | -0.508 35 | 0.107 56 | -0.163 81 | -0.23 | 6 |
S4 | -1.435 74 | -1.229 94 | 0.005 46 | 0.211 82 | -0.81 | 9 |
可溶性糖 Soluble sugar | VC Vitamin C | 游离氨基酸 Free amino acid | 可溶性蛋白 Soluble protein | 硝酸盐 Nitrate | 粗纤维 Crude fiber | |
---|---|---|---|---|---|---|
可溶性糖Soluble sugar | 1.000 0 | 0.403 0* | -0.175 7 | 0.650 0** | -0.514 0** | -0.352 3 |
维生素C Vitamin C | 1.000 0 | -0.187 3 | 0.437 0* | -0.043 6 | -0.601 0** | |
游离氨基酸Free amino acid | 1.000 0 | 0.204 7 | -0.414 0* | 0.087 7 | ||
可溶性蛋白Soluble protein | 1.000 0 | -0.536 0** | -0.321 9 | |||
硝酸盐Nitrate | 1.000 0 | 0.156 6 | ||||
粗纤维Crude fiber | 1.000 0 |
表5 小白菜品质指标间的相关性分析
Table 5 Correlation analysis among the quality of pakchoi
可溶性糖 Soluble sugar | VC Vitamin C | 游离氨基酸 Free amino acid | 可溶性蛋白 Soluble protein | 硝酸盐 Nitrate | 粗纤维 Crude fiber | |
---|---|---|---|---|---|---|
可溶性糖Soluble sugar | 1.000 0 | 0.403 0* | -0.175 7 | 0.650 0** | -0.514 0** | -0.352 3 |
维生素C Vitamin C | 1.000 0 | -0.187 3 | 0.437 0* | -0.043 6 | -0.601 0** | |
游离氨基酸Free amino acid | 1.000 0 | 0.204 7 | -0.414 0* | 0.087 7 | ||
可溶性蛋白Soluble protein | 1.000 0 | -0.536 0** | -0.321 9 | |||
硝酸盐Nitrate | 1.000 0 | 0.156 6 | ||||
粗纤维Crude fiber | 1.000 0 |
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