镉胁迫对不同品种羊肚菌镉富集规律与抗氧化系统的影响

doi:10.3969/j.issn.1004-1524.20221550

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (10): 2321-2331.DOI: 10.3969/j.issn.1004-1524.20221550

镉胁迫对不同品种羊肚菌镉富集规律与抗氧化系统的影响

范丽莹¹^,²(), 范婷婷², 仝宗军³, 梁立韵², 赵志勇², 陈辉³, 周昌艳², 赵晓燕²^,^*()

1.上海理工大学健康科学与工程学院,上海 200093
2.上海市农业科学院农产品质量标准与检测技术研究所,上海 201403
3.上海市农业科学院食用菌研究所,上海 201403

收稿日期:2022-11-03 出版日期:2023-10-25 发布日期:2023-10-31
作者简介:范丽莹(1998—),女,黑龙江牡丹江人,硕士研究生,主要从事食品安全与检测研究。E-mail:affflllyyy111@163.com
通讯作者: ^*赵晓燕,E-mail:cindy8119@163.com
基金资助:
国家农产品质量安全风险评估项目(GJFP20210205)

Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress

FAN Liying¹^,²(), FAN Tingting², TONG Zongjun³, LIANG Liyun², ZHAO Zhiyong², CHEN Hui³, ZHOU Changyan², ZHAO Xiaoyan²^,^*()

1. School of Health Sciences and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
2. Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
3. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received:2022-11-03 Online:2023-10-25 Published:2023-10-31

摘要/Abstract

摘要：

羊肚菌(Morchella spp.)作为一种覆土栽培的食用菌,面临土壤环境镉(Cd)暴露和积累的风险。本研究选取常用栽培品种六妹羊肚菌(Morchella sextelata)M25和梯棱羊肚菌(Morchella importuna)I15作为试验材料,对比分析Cd胁迫对菌丝生理和Cd富集规律的影响,具体探究菌丝生长速度、生物量、微观结构、抗氧化酶、抗氧化活性物质和Cd含量的变化差异。结果显示,I15比M25富集Cd的能力强,不同品种羊肚菌过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)、抗坏血酸过氧化物酶(APX)活性和丙二醛(MDA)、抗坏血酸(AsA)含量均随Cd质量浓度的增加呈先增后减趋势,但I15比M25的GR、谷胱甘肽过氧化物酶(GSH-Px)活性和谷胱甘肽(GSH)含量更高。综合两个羊肚菌品种的生理和Cd富集能力等指标发现,相比六妹羊肚菌M25而言,Cd富集能力更强、生长更旺盛、更不易受镉损害的梯棱羊肚菌I15在谷胱甘肽转化体系中起到的解毒效果更强。研究结果不仅为羊肚菌Cd抗性菌株筛选和含Cd土壤栽培模式的创新奠定了基础,也为真菌生物修复提供了新的见解。

关键词: 羊肚菌, 镉胁迫, 富集, 抗氧化系统, 谷胱甘肽转化体系

Abstract:

Morchella spp., as a kind of edible fungus cultivated in soil, faces the risk of exposure and accumulation of cadmium (Cd) in the soil environment. In this study, the commonly used cultivars Morchella sextelata M25 and Morchella importuna I15 were selected as test materials to compare and analyze the effects of Cd stress on mycelium physiology and Cd accumulation, specifically including the differences in mycelial growth rate, biomass, microstructure, antioxidant enzymes, antioxidant active substances and Cd content. The results showed that I15 had stronger ability of Cd accumulation than M25. The catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX) enzyme activities and malondialdehyde (MDA), ascorbic acid (AsA) contents of the different Morchella spp. showed a trend of first increasing and then decreasing with the increase of Cd concentration, but the GR, glutathione peroxidase (GSH-Px) enzyme activities and glutathione (GSH) contents of I15 were higher than M25. Combining the physiology and Cd accumulation capacity of the different Morchella spp., it found that I15 had stronger accumulation ability, better grow, less damage under Cd stress than M25, also had stronger defense ability against cell damage in the glutathione transformation system. The results of this study not only laid the foundation for the screening of Morchella spp. Cd-resistant strains and the innovation of Cd-containing soil cultivation models, but also provided new insights for fungal bioremediation.

Key words: Morchella, cadmium stress, accumulation, antioxidant enzymes, glutathione transformation system

中图分类号:

S646.9

范丽莹, 范婷婷, 仝宗军, 梁立韵, 赵志勇, 陈辉, 周昌艳, 赵晓燕. 镉胁迫对不同品种羊肚菌镉富集规律与抗氧化系统的影响[J]. 浙江农业学报, 2023, 35(10): 2321-2331.

FAN Liying, FAN Tingting, TONG Zongjun, LIANG Liyun, ZHAO Zhiyong, CHEN Hui, ZHOU Changyan, ZHAO Xiaoyan. Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2321-2331.

图/表 6

图1 Cd胁迫对不同品种羊肚菌菌丝生长速度(a)、生物量(b)、菌落形态(c)、微观形态(d)的影响 I15,梯棱羊杜菌I15; M25,六妹羊肚菌M25。*、**、***分别表示在P<0.05、P<0.01和P<0.001水平差异显著,ns表示差异不显著。下同。

Fig.1 Effects of Cd stress on the growth rate, biomass, growth morphology, and microscopic morphology of the different Morchella spp. Mycelia I15, M.importuna; M25, M.sextelata M25. *, ** and *** mena significant differences at the levels of 0.05, 0.01 and 0.001 respectiviely, ns means not significant. The same as below.

图2 Cd胁迫处理对不同品种羊肚菌菌丝Cd富集能力的影响柱上无相同小写字母表示差异显著。

Fig.2 Effects of Cd stress on the Cd bioconcentration factor of the different Morchella spp. mycelia Data on the bars marked without the same lowercase letter indicated significant differences at P<0.05.

表1 培养基中Cd含量与不同品种羊肚菌菌丝体Cd含量关系的数学模型

Table 1 Mathematical model of relationship between Cd contents in culture medium and the different Morchella spp. mycelia

菌株 Strain	回归方程 regression equation	决定系数 R²
I 15	y=1.797/(1+e^2.891-6.187^x)	0.954 7
M25	y=3.271/(1+e^3.232-2.582^x)	0.983 3

图3 Cd胁迫处理对不同品种羊肚菌菌丝MDA含量的影响

Fig.3 Effects of Cd stress on MDA content in the different Morchella spp. Mycelia

图4 Cd胁迫处理对不同品种羊肚菌菌丝SOD、CAT、APX、GR、GSH-Px的影响

Fig.4 Effects of Cd stress on activities of SOD, CAT, APX, GR and GSH-Px in the mycelia of different Morchella spp.

图5 Cd胁迫处理对羊肚菌菌丝GSH和AsA含量的影响

Fig.5 Effects of Cd stress on the content of GSH and AsA in the mycelia of the different Morchella spp.

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镉胁迫对不同品种羊肚菌镉富集规律与抗氧化系统的影响

Effects on accumulation of cadmium and antioxidant system of different Morchella spp. under cadmium stress

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