利用杂交育种创制耐涝凤丹种质

doi:10.3969/j.issn.1004-1524.20240728

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (11): 2275-2282.DOI: 10.3969/j.issn.1004-1524.20240728

利用杂交育种创制耐涝凤丹种质

刘慧春(), 许雯婷, 周江华, 温小蕙, 史小华, 朱开元()

浙江省园林植物与花卉研究所,浙江杭州 311251

收稿日期:2024-07-25 出版日期:2025-11-25 发布日期:2025-12-08
作者简介:刘慧春(1979—),女,湖北武汉人,博士,副研究员,主要从事江南牡丹资源收集、遗传育种及抗逆性机理研究。E-mail:lhuichun@163.com
通讯作者: ^*朱开元,E-mail: kyzhu1999@163.com
基金资助:
浙江省公益技术应用研究项目(LGN22C150015)

Cultivar development of waterlogging tolerant germplasm of Paeonia ostii cv. Fengdan by hybirid breeding

LIU Huichun(), XU Wenting, ZHOU Jianghua, WEN Xiaohui, SHI Xiaohua, ZHU Kaiyuan()

Zhejiang Institute of Landscaping Plants and Flowers, Hangzhou 311251, China

Received:2024-07-25 Online:2025-11-25 Published:2025-12-08

摘要/Abstract

摘要： 在建成江南牡丹种质资源圃的基础上,利用耐涝性较强的江南观赏牡丹品种与油用牡丹凤丹进行品种间杂交,试图将耐涝的优良基因引入油用牡丹品种中,以培育出耐涝性强的油用牡丹新品种。主要杂交组合有:大富贵×凤丹、玉楼春×凤丹、一品红×凤丹、红芙蓉×凤丹。结果表明:不同的杂交组合,其结实率、种子发芽率和杂交实生苗的耐涝性各不相同,4种杂交组合的结实率都在70%以上,其中玉楼春×凤丹的结实率达到了85%;种子发芽率在60%~80%,其中杂交组合玉楼春×凤丹获得的种子发芽率达到了80%;与凤丹母本相比,杂交获得的实生苗耐涝性均得到了提高,其植株的耐涝性生理指标如蔗糖合成酶(SS)、α-淀粉酶(RAMY)、乙醇脱氢酶(ADH)和丙酮酸脱羧酶(PDC)均显著高于对照凤丹,表现最为明显的是一品红×凤丹,其次是玉楼春×凤丹。本研究结果获得了耐涝的凤丹新种质,为雨水较多的南方地区特别是江南地区提供了品种资源,从而从根本上解决油用牡丹所面临的涝害问题,为油用牡丹的产业发展提供了有力的资源保障。

关键词: 凤丹, 杂交, 结实率, 发芽率, 耐涝性

Abstract:

On the basis of building the Jiangnan Peony Planting Resource Garden, inter-cultivars hybridization was carried out between the Jiangnan ornamental peony variety with strong flood tolerance and the oil peony variety Fengdan, attempting to introduce excellent flood tolerance genes into oil peony varieties to cultivate new oil peony varieties with strong flood tolerance. The main hybrid combinations are as follows: Dafugui×Fengdan, Yulouchun×Fengdan, Yipinhong×Fengdan, Hongfurong×Fengdan. The results showed that different hybrid combinations had different seed setting rates, germination rates, and waterlogging tolerance of hybrid seedlings. The seed setting rates of the four hybrid combinations were all above 70%, with Yulouchun×Fengdan achieving a seed setting rate of 85%. The germination rate of seeds was between 60%-80%, and the hybrid combination Yulouchun×Fengdan achieved a seed germination rate of 80%. Compared with the maternal parent of Fengdan, the waterlogging tolerance of the hybrid seedlings has been improved. The physiological indicators of waterlogging tolerance, such as sucrose synthase (SS),α-amylase (RAMY), alcohol dehydrogenase (ADH), and pyruvate decarboxylase (PDC), were significantly higher than those in the control Fengdan, with the most obvious improvement observed in Yipinhong×Fengdan, followed by Yulouchun×Fengdan. The results of this study have obtained a new germplasm of Fengdan that is tolerant to waterlogging, providing a variety resource for the southern regions with more rainfall, especially the Jiangnan region. It fundamentally solves the problem of waterlogging faced by oil peonies and provides favorable resource guarantees for industrial development of oil peonies.

Key words: Paeonia ostii cv. Fengdan, hybridization, seed setting rate, germination rate, waterlogging tolerance

中图分类号:

S685.11

刘慧春, 许雯婷, 周江华, 温小蕙, 史小华, 朱开元. 利用杂交育种创制耐涝凤丹种质[J]. 浙江农业学报, 2025, 37(11): 2275-2282.

LIU Huichun, XU Wenting, ZHOU Jianghua, WEN Xiaohui, SHI Xiaohua, ZHU Kaiyuan. Cultivar development of waterlogging tolerant germplasm of Paeonia ostii cv. Fengdan by hybirid breeding[J]. Acta Agriculturae Zhejiangensis, 2025, 37(11): 2275-2282.

图/表 5

参考文献 30

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杂交组合 Hybrid combination	授粉数量 Pollination quantity	果荚数量 Number of fruit pods	种子数量 Number of seeds	种子质量 Weight of seeds/g	结实率 Seed setting rate/%	单粒重 Single seed weight/g
DFG×FD	50	35	989	440.97	70.00	0.445 9
YLC×FD	100	85	2 512	1 113.58	85.00	0.443 3
YPH×FD	60	43	1 395	621.38	71.66	0.445 4
HFR×FD	80	60	896	416.36	75.00	0.464 7

杂交组合 Hybrid combination	授粉数量 Pollination quantity	果荚数量 Number of fruit pods	种子数量 Number of seeds	种子质量 Weight of seeds/g	结实率 Seed setting rate/%	单粒重 Single seed weight/g
DFG×FD	50	35	989	440.97	70.00	0.445 9
YLC×FD	100	85	2 512	1 113.58	85.00	0.443 3
YPH×FD	60	43	1 395	621.38	71.66	0.445 4
HFR×FD	80	60	896	416.36	75.00	0.464 7

杂交组合 Hybrid combination	播种数量 Sowing quantity	出苗数量 Number of emerging seedlings	发芽率 Germination rate/%
DFG×FD	150	91	60.67
YLC×FD	150	120	80.00
YPH×FD	150	118	78.67
HFR×FD	150	93	62.00

杂交组合 Hybrid combination	播种数量 Sowing quantity	出苗数量 Number of emerging seedlings	发芽率 Germination rate/%
DFG×FD	150	91	60.67
YLC×FD	150	120	80.00
YPH×FD	150	118	78.67
HFR×FD	150	93	62.00

杂交组合 Hybrid combination	组别 Group	初始株高 Initial height/ cm	最终株高 Final height/ cm	叶绿素相对含量 Single photon avalanche diode	根系长度 Root length/cm	根冠比 Root/shoot ratio
YLC×FD	涝害处理组Treatment group	11.5	11.5	5.26	16.00	1.46
	对照组Control group	11.5	11.8	5.24	21.17	1.85
YPH×FD	涝害处理组Treatment group	11.0	11.0	7.20	17.50	1.56
	对照组Control group	11.0	11.2	6.40	24.00	1.80
FD	涝害处理组Treatment group	12.0	12.0	5.98	20.33	1.29
	对照组Control group	12.0	12.3	6.54	23.17	1.88

利用杂交育种创制耐涝凤丹种质

Cultivar development of waterlogging tolerant germplasm of Paeonia ostii cv. Fengdan by hybirid breeding

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