浙江农业学报

• 动物科学 • 上一篇    下一篇

中华鳖肠道及其养殖水体菌群比较

  

  1. (浙江省水产技术推广总站,浙江 杭州 310012)
  • 出版日期:2014-09-25 发布日期:2014-10-11

Comparison of bacteria floras in intestines of healthy and diseased Chinese soft\|shelled turtles (Pelodiscus sinensis) and in aquatic water

  1. (Zhejiang Fisheries Technical Extension Center, Hangzhou 310012, China)
  • Online:2014-09-25 Published:2014-10-11

摘要: 为了解养殖水体环境对中华鳖的影响,通过平板计数及细菌的分离鉴定,对健康鳖肠道、患病鳖肠道以及其养殖水体中菌群的数量、种类等进行比较分析。研究显示,病鳖肠道菌群总量(121×105)~(49×107)cfu·g-1,明显高于健康鳖的(32×104)~(713×105)cfu·g-1,且菌群种类较健康鳖多样;健康鳖肠道优势菌为肠球菌与柠檬酸杆菌,患病鳖肠道优势菌为柠檬酸杆菌、芽孢杆菌、气单胞菌;养殖水体中分离到的细菌均在病鳖肠道内分离到,从一定程度上反映了养殖环境对其肠道菌群的重要影响。

关键词: 中华鳖, 肠道菌群, 养殖水体, 分离鉴定

Abstract: In order to clarify the effects of aquatic environment on Chinese soft\|shelled turtles (Pelodiscus sinensis), the bacterial floras in intestines of healthy and diseased turtles and in the aquatic water were investigated. The results showed that the total bacterial numbers in intestines of healthy and diseased turtles were 32×104~713×105 cfu·g-1 and 121×105~49×107 cfu·g-1, respectively. The species of intestinal bacterial flora of diseased turtles were more complicated than the healthy ones. Enterococcus and Citrobacter were the dominant bacterial species in healthy turtles, while Citrobacte, Aeromonas and Bacillus dominated in the diseased ones. All the bacterial species isolated from aquatic water could be found in the diseased turtles, which indicated the important influence of the aquatic environment on the growth of organisms.

Key words: Pelodiscus sinensis, intestinal bacteria flora, aquatic water, isolation and identification