关键词: 南瓜, 耐盐性, RAPD, SSR

Abstract: In this study， salt\|tolerant pumpkin NM083 and salt\|susceptive pumpkin XBZM were screened by salt tolerance test， and the seedling emergence rate and salt injury index were used as the salt tolerance identification index. The F2 segregated population originating from a cross between pumpkin inbreed NM083 （male parent， salt\|tolerant） and XBZM （female parent， salt\|susceptive） was constructed. Among 600 RAPD and 44 SSR primers， a total number of 18 markers （10 RAPD and 8 SSR） showed the polymorphism between two parents， and the ratio of polymorphism RAPD markers and SSR markers in two parents was 043% and 43%， respectively. Among 18 markers， one RAPD primer（P597） was screened out， which could amplify a polymorphic locus （685 bp） between two parents and between salt\|tolerant and salt\|susceptive bulks.The salt\|tolerant test in 160 F2 segregated populations showed that the polymorphic locus （685 bp） appeared in salt\|tolerant F2 segregated population but not in salt\|susceptive F2 segregated population. It could be concluded that this band was the RAPD marker closely related with salt tolerance of pumpkin， which could be used as the effective marker in molecular assisted selection of new varieties breeding of salt\|tolerant pumpkin.

Key words: pumpkin, salt tolerance, RAPD, SSR