c-GMP诱导对盐胁迫下番茄的转录组分析

doi:10.3969/j.issn.1004-1524.2020.10.07

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (10): 1788-1797.DOI: 10.3969/j.issn.1004-1524.2020.10.07

c-GMP诱导对盐胁迫下番茄的转录组分析

朱晓林^1,2,3, 魏小红^1,2,*, 王宝强^1,2,3, 王贤^1,2, 张明君^2,3

1.甘肃农业大学生命科学技术学院,甘肃兰州 730070;
2.甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;
3.甘肃省干旱生境作物学重点实验室,甘肃兰州 730070

收稿日期:2020-01-18 出版日期:2020-10-25 发布日期:2020-11-16
通讯作者: *魏小红,E-mail: 1981263554@qq.com
作者简介:朱晓林(1996—),男,甘肃临潭人,硕士研究生,研究方向为植物生态生理。E-mail:2428498183@qq.com
基金资助:
国家自然科学基金(31560663)

Transcriptome analysis of tomato under salt stress induced by c-GMP

ZHU Xiaolin^1,2,3, WEI Xiaohong^1,2,*, WANG Baoqiang^1,2,3, WANG Xian^1,2, ZHANG Mingjun^2,3

1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Gansu Key Lab of Crop Genetic & Germplasm Enhancement, Lanzhou 730070, China;
3. Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou 730070, China

Received:2020-01-18 Online:2020-10-25 Published:2020-11-16

摘要/Abstract

摘要： 以市场常见的番茄为材料,采用Illumina高通量测序技术对NaCl胁迫、c-GMP诱导以及两者组合处理下番茄幼苗进行转录组测序。结果表明:共获得83.35 Gb的原始数据,Q30碱基百分比在90.91%及以上,分别将各样品的原始读数与指定的参考基因组进行序列比对,比对效率从88.03%到92.74%不等。同时将得到的31 734条序列注释到不同的数据库(GO、KEGG、KOG、NR、Pfam、Swiss-Prot和egg NOG),通过GO数据库,将其划分到细胞组分、分子功能以及生物学过程三大注释类别下的51个亚功能组中,通过COG数据库与KEGG数据库将其分别注释到25个类别与128个代谢通路。在NaCl胁迫、c-GMP诱导以及两者组合处理下共发现SNP位点127 944个,其中仅有22.6%的SNP位点位于非编码区。差异基因结果显示,c-GMP能诱导盐胁迫下更多基因的表达。该转录组测序数据可靠,结果覆盖面广,为番茄c-GMP诱导盐胁迫下基因挖掘与功能研究提供了理论依据。

关键词: 番茄, 盐胁迫, c-GMP诱导, 转录组分析

Abstract: Using tomato as material, Illumina high-throughput sequence technology was used to sequence the transcriptome of tomato seedlings under NaCl stress, c-GMP induction and the combination of the two. The results showed that: a total of 83.35 Gb of original data were obtained, and the percentage of Q30 bases was 90.91% and above. The original reading of each sample was compared with the designated reference genome, and the comparison efficiency ranged from 88.03% to 92.74%. At the same time, the 31 734 sequences obtained were annotated to different databases (GO, KEGG, KOG, NR, Pfam, Swiss-Prot and egg NOG), and they were divided into cell component, molecular function and biological process through the GO database. Among the 51 subfunctional groups under the big annotation category, they were annotated into 25 categories and 128 metabolic pathways through the COG database and the KEGG database, respectively. A total of 127 944 SNP sites were found under NaCl stress, c-GMP induction and the combination of the two treatments, of which only 22.6% of SNP sites were located in the non-coding region. The differential gene results showed that c-GMP could induce the expression of more genes under salt stress. The transcriptome sequence data was reliable and the results covered a wide range, providing a theoretical basis for gene mining and functional research of tomato under salt stress induced by c-GMP.

Key words: tomato, salt stress, c-GMP induction, transcriptome analysis

中图分类号:

S641.2

朱晓林, 魏小红, 王宝强, 王贤, 张明君. c-GMP诱导对盐胁迫下番茄的转录组分析[J]. 浙江农业学报, 2020, 32(10): 1788-1797.

ZHU Xiaolin, WEI Xiaohong, WANG Baoqiang, WANG Xian, ZHANG Mingjun. Transcriptome analysis of tomato under salt stress induced by c-GMP[J]. , 2020, 32(10): 1788-1797.

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c-GMP诱导对盐胁迫下番茄的转录组分析

Transcriptome analysis of tomato under salt stress induced by c-GMP

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