海藻糖处理对花后高温胁迫弱筋小麦生选6号产量形成及品质的影响

doi:10.3969/j.issn.1004-1524.2023.01.01

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (1): 1-9.DOI: 10.3969/j.issn.1004-1524.2023.01.01

海藻糖处理对花后高温胁迫弱筋小麦生选6号产量形成及品质的影响

王犇(), 李宇星, 李哲, 姜沣溢, 黄正来(), 樊永惠, 张文静, 马尚宇

安徽农业大学农学院/农业农村部黄淮南部小麦生物学与遗传育种重点实验室,安徽合肥 230036

收稿日期:2021-09-30 出版日期:2023-01-25 发布日期:2023-02-21
通讯作者: *黄正来,E-mail:xdnyyjs@163.com
作者简介:王犇(1997—),男,辽宁朝阳人,硕士研究生,研究方向为作物栽培生理生态。E-mail:1021656439@qq.com
基金资助:
安徽省科技重大专项(S202003a06020014)

Performance of trehalose treatment on yield formation and quality of post-flowering heat-stressed weak gluten wheat Shengxuan No.6

WANG Ben(), LI Yuxing, LI Zhe, JIANG Fengyi, HUANG Zhenglai(), FAN Yonghui, ZHANG Wenjing, MA Shangyu

College of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow & Huai River Valley, Ministry of Agriculture and Rural Affairs, Hefei 230036, China

Received:2021-09-30 Online:2023-01-25 Published:2023-02-21

摘要/Abstract

摘要：

为明确海藻糖处理对花后高温胁迫弱筋小麦产量形成及品质的影响,试验于2020—2021年在安徽省淮南市国家农业科技园区进行,以优质弱筋小麦生选6号为供试材料,在抽穗期(T1)、开花期(T2)、花后10 d(T3)进行海藻糖处理,处理浓度为5 mmol·L^-1(C1)、10 mmol·L^-1(C2)、15 mmol·L^-1(C3),以喷施等量清水为对照,花后15~19 d进行高温处理。结果表明,海藻糖处理可以提高花后高温胁迫下小麦籽粒产量,以及籽粒干物质分配量和比例,海藻糖处理后营养器官干物质分配量及占比呈现下降趋势,而花前营养器官储藏的干物质向籽粒中的转运量及转运率、花后干物质积累量表现出增加的趋势。随着海藻糖处理浓度的增加籽粒淀粉含量也呈现出增加的趋势,不同处理时期C2、C3两个处理水平间无显著差异,但C3要略高于C2。海藻糖处理降低了籽粒清蛋白、球蛋白、醇溶蛋白含量,但谷蛋白含量高于CK,并且海藻糖处理下籽粒谷/醇值显著高于CK。海藻糖处理降低了籽粒蛋白质含量、湿面筋含量、形成时间、稳定时间及沉降值,且与CK有显著差异。在本试验研究条件下T1时期喷施C3浓度海藻糖处理的籽粒产量及品质较CK提升最大。

关键词: 高温胁迫, 弱筋小麦, 海藻糖, 产量形成, 品质

Abstract:

To determine the effect of treatment with trehalose on yield formation and quality of post-flowering heat-stressed weak gluten wheat, the experiment was conducted in 2020-2021 at the National Agricultural Science and Technology Park in Huainan, Anhui Province, with high-quality weakly glutenous wheat Shengxuan No. 6 as the test material. The experiment was carried out at heading stage (T1), flowering stage (T2) and 10 days after flowering stage (T3). Treatment concentrations of trehalose were 5 mmol·L^-1 (C1), 10 mmol·L^-1 (C2), and 15 mmol·L^-1 (C3), with equal amounts of water sprayed as control, high temperature treatment was applied 15-19 d after flowering. The analysis showed that trehalose treatment increased grain yield of wheat under post-anthesis high temperature stress and improved the dry matter allocation and proportion of grains, and the dry matter allocation and proportion of nutrient organs displayed a decreasing trend after trehalose treatment, while the transport amount and rate of dry matter stored in nutrient organs before flowering to the grains and the accumulation of dry matter after flowering showed an increasing trend. With the increasing concentration of trehalose treatment the starch content of the grains also showed an increasing trend, and no significant difference was found between the two treatment levels of C2 and C3 in various treatment periods, however, the level of C3 was slightly higher than C2. Trehalose treatment reduced the content of grain albumin, globulin and gliadin protein, but the content of glutenin was higher than CK, and the glutein/gliadin value of the grains under trehalose treatment was significantly higher than CK. The trehalose-treated kernels were significantly different from CK in terms of protein content, wet gluten content, formation time, stabilization time and sedimentation value. The greatest improvement in grain yield and quality was achieved by spraying C3 concentration of trehalose at T1 period compared with CK under the study conditions of this experiment.

Key words: high temperature stress, weakly glutenous wheat, trehalose, yield formation, quality

中图分类号:

S512.1

王犇, 李宇星, 李哲, 姜沣溢, 黄正来, 樊永惠, 张文静, 马尚宇. 海藻糖处理对花后高温胁迫弱筋小麦生选6号产量形成及品质的影响[J]. 浙江农业学报, 2023, 35(1): 1-9.

WANG Ben, LI Yuxing, LI Zhe, JIANG Fengyi, HUANG Zhenglai, FAN Yonghui, ZHANG Wenjing, MA Shangyu. Performance of trehalose treatment on yield formation and quality of post-flowering heat-stressed weak gluten wheat Shengxuan No.6[J]. Acta Agriculturae Zhejiangensis, 2023, 35(1): 1-9.

图/表 10

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花后天数 Day after anthesis/d	处理时平均温 Average temperature at treatment/℃	处理时棚外平均温 Control mean temperature at treatment/℃	温度差 Temperature difference/℃
15	28.1	18.8	9.3
16	29.5	20.4	9.1
17	32.5	24.4	8.1
18	31.0	20.7	10.3
19	29.3	20.1	9.2

花后天数 Day after anthesis/d	处理时平均温 Average temperature at treatment/℃	处理时棚外平均温 Control mean temperature at treatment/℃	温度差 Temperature difference/℃
15	28.1	18.8	9.3
16	29.5	20.4	9.1
17	32.5	24.4	8.1
18	31.0	20.7	10.3
19	29.3	20.1	9.2

喷施时期 Application period	处理 Treatment	籽粒 Grain		茎鞘+叶片 Steam and sheath and leaf		颖壳+穗轴 Glume and spike axis
喷施时期 Application period	处理 Treatment	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%
T1	CK	1.20 f	41.86 d	1.28 a	43.87 a	0.38 c	13.27 d
	C1	1.26 e	43.40 c	1.28 a	43.49 c	0.35 e	12.15 i
	C2	1.34 b	44.96 b	1.26 b	41.69 g	0.37 d	12.42 h
	C3	1.43 a	47.49 a	1.23 e	41.11 i	0.34 f	11.29 l
T2	CK	1.19 f	42.78 cd	1.24 d	43.72 b	0.35 e	12.52 f
	C1	1.20 f	41.84 d	1.25 c	43.10 d	0.40 b	14.10 b
	C2	1.27 e	45.23 b	1.19 g	41.39 h	0.35 e	12.47 g
	C3	1.33 bc	47.27 a	1.02 h	35.78 l	0.45 a	16.16 a
T3	CK	1.20 f	42.49 cd	1.22 e	42.55 e	0.39 b	13.99 c
	C1	1.27 de	45.55 b	1.19 f	41.82 f	0.33 g	11.72 k
	C2	1.33 bc	46.56 a	1.18 g	40.54 k	0.34 f	12.00 j
	C3	1.30 cd	45.37 b	1.20 f	40.97 j	0.37 d	12.77 e
T		31.59^**	5.59^*	2 236.46^**	2 398.50^**	527.80^**	30 965.22^**
C		137.58^**	111.68^**	1 649.05^**	9 724.94^**	327.29^**	9 749.92^**
T×C		12.57^**	17.09^**	779.78^**	2 674.30^**	722.73^**	23 581.74^**

喷施时期 Application period	处理 Treatment	籽粒 Grain		茎鞘+叶片 Steam and sheath and leaf		颖壳+穗轴 Glume and spike axis
喷施时期 Application period	处理 Treatment	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%	分配量 Allocation volume/g	比例 Proportion/%
T1	CK	1.20 f	41.86 d	1.28 a	43.87 a	0.38 c	13.27 d
	C1	1.26 e	43.40 c	1.28 a	43.49 c	0.35 e	12.15 i
	C2	1.34 b	44.96 b	1.26 b	41.69 g	0.37 d	12.42 h
	C3	1.43 a	47.49 a	1.23 e	41.11 i	0.34 f	11.29 l
T2	CK	1.19 f	42.78 cd	1.24 d	43.72 b	0.35 e	12.52 f
	C1	1.20 f	41.84 d	1.25 c	43.10 d	0.40 b	14.10 b
	C2	1.27 e	45.23 b	1.19 g	41.39 h	0.35 e	12.47 g
	C3	1.33 bc	47.27 a	1.02 h	35.78 l	0.45 a	16.16 a
T3	CK	1.20 f	42.49 cd	1.22 e	42.55 e	0.39 b	13.99 c
	C1	1.27 de	45.55 b	1.19 f	41.82 f	0.33 g	11.72 k
	C2	1.33 bc	46.56 a	1.18 g	40.54 k	0.34 f	12.00 j
	C3	1.30 cd	45.37 b	1.20 f	40.97 j	0.37 d	12.77 e
T		31.59^**	5.59^*	2 236.46^**	2 398.50^**	527.80^**	30 965.22^**
C		137.58^**	111.68^**	1 649.05^**	9 724.94^**	327.29^**	9 749.92^**
T×C		12.57^**	17.09^**	779.78^**	2 674.30^**	722.73^**	23 581.74^**

喷施时期 Application period	处理 Treatment	花前干物质转运 Pre-anthesis dry matter translocation			花后干物质积累 Post-anthesis dry matter accumulation
喷施时期 Application period	处理 Treatment	转运量 Translocation into grain/(kg·hm^-2)	转运率 Translocation ratio/%	对籽粒干物质贡献率 Contribution to grain/%	积累量 Assimilation amount/(kg·hm^-2)	对籽粒干物质贡献率 Contribution to grain/%
T1	CK	2 724.95 j	20.49 j	33.39 i	5 438.06 c	66.61 b
	C1	2 878.55 i	21.57 i	33.53 hi	5 708.35 b	66.47 bc
	C2	2 892.10 i	21.66 i	31.71 j	6 229.67 a	68.29 a
	C3	3 352.88 f	24.90 f	34.33 h	6 422.11 a	65.67 c
喷施时期 Application period	处理 Treatment	花前干物质转运 Pre-anthesis dry matter translocation			花后干物质积累 Post-anthesis dry matter accumulation
喷施时期 Application period	处理 Treatment	转运量 Translocation into grain/(kg·hm^-2)	转运率 Translocation ratio/%	对籽粒干物质贡献率 Contribution to grain/%	积累量 Assimilation amount/(kg·hm^-2)	对籽粒干物质贡献率 Contribution to grain/%
T2	CK	3 181.22 g	23.70 g	39.13 e	4 950.75 d	60.87 f
	C1	2 738.51 j	20.58 j	33.57 hi	5 420.39 c	66.43 bc
	C2	3 560.68 d	26.36 d	41.09 c	5 106.94 d	58.91 h
	C3	3 967.25 a	29.22 a	43.79 a	5 093.91 d	56.21 j
T3	CK	2 996.00 h	22.39 h	36.66 g	5 178.66 d	63.34 d
	C1	3 678.13 b	27.19 b	42.29 b	5 020.58 d	57.71 i
	C2	3 641.99 c	26.93 c	40.14 d	5 433.20 c	59.86 g
	C3	3 379.98 e	25.09 e	38.05 f	5 503.81 bc	61.95 e
T		5 075.23^**	3 896.35^**	670.48^**	146.17^**	670.38^**
C		4 368.38^**	3 353.54^**	49.66^**	27.89^**	49.65^**
T×C		2 342.76^**	1 798.68^**	150.84^**	15.27^**	150.82^**

海藻糖处理对花后高温胁迫弱筋小麦生选6号产量形成及品质的影响

Performance of trehalose treatment on yield formation and quality of post-flowering heat-stressed weak gluten wheat Shengxuan No.6

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喷施时期 Application period	处理 Treatment	清蛋白 Albumin/%	球蛋白 Globulin/%	醇溶蛋白 Gliadin/%	谷蛋白 Glutein/%	谷/醇 Glutein/gliadin
T1	CK	3.19±0.01 a	2.37±0.01 a	2.34±0.06 a	2.80±0.03 i	1.20±0.03 g
	C1	2.97±0.04 b	2.07±0.01 e	1.94±0.05 b	3.00±0.03 f	1.55±0.03 f
	C2	2.83±0.04 cd	1.92±0.01 h	1.72±0.06 d	3.10±0.03 c	1.81±0.06 d
	C3	2.77±0.04 d	1.77±0.01 j	1.52±0.06 f	3.20±0.03 a	2.12±0.08 a
T2	CK	3.19±0.01 a	2.34±0.02 b	2.34±0.06 a	2.80±0.03 i	1.20±0.03 g
	C1	3.01±0.04 b	2.17±0.01 c	1.96±0.06 b	2.95±0.03 h	1.50±0.04 f
	C2	2.88±0.03 c	2.06±0.01 e	1.79±0.05 c	3.02±0.03 e	1.69±0.04 e
	C3	2.80±0.04 cd	1.97±0.01 g	1.62±0.04 e	3.10±0.03 c	1.92±0.03 c
T3	CK	3.19±0.01 a	2.37±0.01 a	2.34±0.06 a	2.80±0.03 i	1.20±0.03 g
	C1	2.98±0.04 b	2.12±0.01 d	1.95±0.06 b	2.97±0.03 g	1.53±0.04 f
	C2	2.85±0.04 cd	2.00±0.01 f	1.75±0.06 d	3.06±0.03 d	1.75±0.05 d
	C3	2.79±0.04 d	1.87±0.01 i	1.59±0.04 e	3.15±0.03 b	1.98±0.03 b
T		1.13	154.34^**	16.39^**	1 290.30^**	21.24^**
C		151.54^**	1 907.43^**	2 329.44^**	27 761.85^**	950.76^**
T×C		0.15	33.38^**	4.10^**	186.63^**	5.50^**

喷施时期 Application period	处理 Treatment	蛋白质 Protein content/%	容重 Bulk density/g	湿面筋 Wet gluten/%	形成时间 Formation time/min	稳定时间 Stabilization time/min	沉降值 Sedimentation value/mL
T1	CK	12.76±0.09 a	792.00±1.25 bc	26.14±0.17 a	7.60±0.05 a	2.87±0.03 a	20.53±0.50 a
	C1	12.16±0.09 c	790.67±0.27 bcde	23.58±0.17 c	6.90±0.14 b	2.27±0.03 c	16.47±0.05 b
	C2	11.86±0.09 e	788.00±1.63 e	21.69±0.17 e	6.07±0.03 cd	1.97±0.03 e	14.17±0.17 c
	C3	11.58±0.05 g	792.00±0.47 bc	21.37±0.18 g	5.37±0.19 e	1.70±0.05 g	12.50±0.08 d
T2	CK	12.76±0.09 a	791.67±1.19 bcd	26.14±0.17 a	7.60±0.05 a	2.87±0.03 a	20.53±0.50 a
	C1	12.22±0.09 b	792.00±1.25 bc	23.68±0.17 b	7.05±0.14 b	2.39±0.03 b	16.62±0.05 b
	C2	11.93±0.09 d	788.33±0.54 cde	22.44±0.17 d	6.28±0.03 c	2.15±0.03 d	14.33±0.17 c
	C3	11.79±0.09 f	791.00±0.00 bcde	21.68±0.17 e	5.59±0.19 e	1.91±0.03 ef	12.64±0.08 d
T3	CK	12.76±0.09 a	799.00±0.00 a	26.11±0.19 a	7.57±0.05 a	2.87±0.03 a	20.53±0.50 a
	C1	12.20±0.09 bc	791.67±1.19 bcd	23.75±0.19 b	7.02±0.14 b	2.36±0.03 b	16.59±0.05 b
	C2	11.93±0.09 d	792.33±0.98 b	22.45±0.19 d	6.30±0.03 c	2.10±0.03 d	14.33±0.17 c
	C3	11.75±0.09 f	794.33±0.72 b	21.58±0.19 f	5.73±0.05 de	1.85±0.03 f	12.62±0.08 d
T		31.90^**	14.49^**	109.87^**	2.32	26.40^**	0.18
C		2536.05^**	9.42^**	12 625.54^**	160.95^**	933.22^**	421.18^**
T×C		8.46^**	2.44^*	39.40^**	0.52	3.46^*	0.02

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