浙江农业学报 ›› 2023, Vol. 35 ›› Issue (2): 338-345.DOI: 10.3969/j.issn.1004-1524.2023.02.11

• 园艺科学 • 上一篇    下一篇

葫芦科作物叶片硅含量测定方法的优化

孙楠(), 闫国超, 何勇, 朱祝军()   

  1. 浙江农林大学 园艺科学学院,浙江 杭州 311300
  • 收稿日期:2022-04-09 出版日期:2023-02-25 发布日期:2023-03-14
  • 通讯作者: 朱祝军
  • 作者简介:*朱祝军,E-mail:zhuzj@zafu.edu.cn
    孙楠(1991—),男,浙江宁波人,硕士研究生,主要从事蔬菜栽培生理研究。E-mail:2019601041027@stu.zafu.edu.cn
  • 基金资助:
    国家重点研发计划(2018YFD1000806-09)

Rapid determination of silicon content in Cucurbitaceae leaves by silicon molybdenum blue method

SUN Nan(), YAN Guochao, HE Yong, ZHU Zhujun()   

  1. College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China
  • Received:2022-04-09 Online:2023-02-25 Published:2023-03-14
  • Contact: ZHU Zhujun

摘要:

为优化葫芦科作物硅含量的测定方法,给园艺植物硅肥应用与硅营养研究提供技术支持,以‘津优1号’黄瓜、‘蜜本’南瓜和‘早佳8424’西瓜3种葫芦科作物为研究材料,分析硅提取与含量测定过程中不同的研磨温度、研磨时间、浸提时间、硅钼黄显色反应水浴时间、硅钼蓝还原反应时间、显色液吸光度测定波长等对作物叶片硅含量测定结果的影响。结果表明:在植物样品硅提取过程中,研磨仪低温(液氮,-196 ℃)研磨硅含量测定值高于室温研磨。在样品研磨时间与浸提时间方面,正交试验与全因子试验结果均显示,50 Hz低温研磨30 s、浸提10 min组合测定效果最佳。在硅显色测定方面,硅钼黄显色反应水浴3 min后测定值趋于稳定,硅钼蓝还原反应2 min后测定结果趋于稳定,硅钼蓝显色反应液在波长810 nm附近出现最大吸收峰。针对葫芦科作物叶片硅含量的最优测定流程为植物样品采用研磨仪50 Hz液氮研磨30 s,常温浸提10 min,50 ℃水浴3 min进行硅钼黄显色反应,硅钼蓝还原反应2 min,810 nm处测定吸光度,该方法回收率为93%~108%。

关键词: 硅钼蓝比色法, 葫芦科, 叶片, 硅, 研磨温度, 浸提时间, 波长

Abstract:

In this study, the method of silicon content determination in Cucurbitaceae crop tissues was modified aiming to provide technical support for the application of silicon fertilizer and related researches in horticultural plants. Different Cucurbitaceae crops including cucumber (Jinyou No.1), pumpkin (Miben) and watermelon (Zaojia 8424) were used in this study, and the effects of grinding temperature, grinding time, extracting time, water bathing time, reduction reaction time and absorption wavelength on the determination of silicon contents were investigated. As for silicon extraction process, measured value of silicon content by grinding under low-temperature (liquid nitrogen,-196 ℃) showed significant higher than that under room temperature. The results of the orthogonal test and full factorial experiments showed that the combination of low temperature grinding at 50 Hz for 30 s and extracting for 10 min was the best assay. The measured value tended to be stable after 3 min of water bathing, 2 min of reduction reaction. In addition, the results showed that the maximum absorption of silicon molybdenum blue was 810 nm. In conclusion, the optimal methods for silicon extraction and determination were as follows: 50 Hz grinding at low-temperature (liquid nitrogen,-196 ℃) for 30 s, extracting for 10 min at room temperature, 50 ℃ water bathing for 3 min, reduction reaction for 2 min, and the absorbance value was determined at 810 nm using spectrophotometer. The recovery rate of this method was 93%-108%.

Key words: silicon molybdenum blue, Cucurbitaceae, leaf, silicon, grinding temperature, extracting time, wavelength

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