绵羊GRM1基因多态性及其与肉质性状的相关性

doi:10.3969/j.issn.1004-1524.2023.04.07

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (4): 799-808.DOI: 10.3969/j.issn.1004-1524.2023.04.07

绵羊GRM1基因多态性及其与肉质性状的相关性

聂玮(), 孟科, 荣轩, 强浩, 郭晨浩, 陶毛孩, 冯登侦()

宁夏大学农学院,宁夏银川 750021

收稿日期:2022-05-26 出版日期:2023-04-25 发布日期:2023-05-05
通讯作者: ^*冯登侦,E-mail: fengdengzhen126@163.com
作者简介:聂玮(1999—),男,天津武清人,硕士研究生,主要从事动物遗传育种研究。E-mail: nienyle@foxmail.com
基金资助:
宁夏回族自治区科学基金(2020AAC03082);宁夏回族自治区农业育种专项(NXNYYZ20150101)

Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep

NIE Wei(), MENG Ke, RONG Xuan, QIANG Hao, GUO Chenhao, TAO Maohai, FENG Dengzhen()

College of Agriculture, Ningxia University, Yinchuan 750021, China

Received:2022-05-26 Online:2023-04-25 Published:2023-05-05

摘要/Abstract

摘要：

为探究绵羊代谢型谷氨酸受体1基因(GRM1)多态性及其对绵羊肉质性状的影响,以期为绵羊肉质性状选育提供有效的遗传分子标记。采用液相捕获测序技术对滩羊(T)、小尾寒羊(XH)、杜泊羊(D)3个绵羊群体共91只绵羊的GRM1基因进行测序,筛选出2个多态位点rs403075278和rs415006419。利用飞行质谱检测技术对滩羊(T)、小尾寒羊(XH)、杜泊羊(D)和杜滩寒三元杂交羊(DTH)共30只绵羊进行基因分型,对筛选出的位点与肉质性状进行关联分析。结果显示:不同基因型绵羊的肉质性状存在显著差异,GRM1基因rs415006419位点的CT基因型绵羊肾的质量显著(P<0.05)大于TT基因型,TT基因型绵羊肌肉的酪氨酸含量显著(P<0.05)高于CT基因型。rs403075278位点TT基因型绵羊的宰前活重、胴体重、净肉重、十二指肠长度,以及心、肺和肾的质量均极显著(P<0.01)大于CT基因型;TT基因型绵羊的背膘厚、净肉重、脾的质量、肌肉的脂肪、钙含量、苯丙氨酸和组氨酸含量显著(P<0.05)高于CT基因型;CT基因型绵羊的pH值、剪切力和失水率极显著(P<0.01)大于TT基因型;CT和TT基因型的其余屠宰性能、肉品质和肌肉营养成分含量均无显著差异(P>0.05)。以上结果表明,GRM1基因可以作为绵羊肉质性状的候选基因,rs415006419和rs403075278位点可以作为绵羊生长发育和产肉性能的候选遗传标记。

关键词: 绵羊, 代谢型谷氨酸受体1, 多态性, 屠宰性能, 肉质, 营养物质

Abstract:

To investigate the polymorphism of metabotropic glutamate receptor 1 gene (GRM1) in sheep and its effect on meat quality traits, with a view to providing effective genetic molecular markers for the breeding of sheep for meat performance. The GRM1 gene was sequenced in 91 sheep from three sheep populations, Tan sheep (T), Little-tailed Han sheep (XH), and Dorper sheep (D), using liquid-phase capture sequencing technology. The genotypes of the genotyped loci were analyzed by flight mass spectrometry for the meat quality traits of the Tan sheep (T), Little-tailed Han sheep (XH), Dorper sheep (D) and Dutanhan sheep (DTH). The results showed that there were significant differences in meat quality traits among the different genotypes of sheep. Sheep kidney mass with CT genotype was significantly (P<0.05) greater than that with TT genotype at the rs415006419 locus of the GRM1 gene, and tyrosine content in muscle of sheep with TT genotype was significantly (P<0.05) higher than that with CT genotype; Sheep live weight before slaughter, carcass weight, net meat weight, duodenal length, and heart, lung and kidney mass with TT genotype at locus rs403075278 of the GRM1 gene were significantly (P<0.01) greater than those with the CT genotype, and backfat thickness, net meat weight, spleen mass, muscle fat, calcium content, phenylalanine and histidine content with TT genotype were significantly (P<0.05) higher than those with CT genotype. pH, shear and water loss with CT genotype were significantly (P<0.01) greater than those with TT genotype. The remaining slaughter performance, meat quality and muscle nutrient content were not significantly (P>0.05) different between the two genotypes. The above results suggest that GRM1 gene could be used as a candidate gene for meat quality traits in sheep, rs415006419 and rs403075278 loci could be used as potential candidate genetic markers for sheep growth and development, and meat production performance.

Key words: sheep, metabotropic glutamate receptor 1, polymorphism, slaughter performance, meat trait, nutrient

中图分类号:

S826

聂玮, 孟科, 荣轩, 强浩, 郭晨浩, 陶毛孩, 冯登侦. 绵羊GRM1基因多态性及其与肉质性状的相关性[J]. 浙江农业学报, 2023, 35(4): 799-808.

NIE Wei, MENG Ke, RONG Xuan, QIANG Hao, GUO Chenhao, TAO Maohai, FENG Dengzhen. Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep[J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 799-808.

图/表 9

参考文献 36

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SNPs	突变类型 Mutation type	上游引物 Forward primer(5’→3’)	下游引物 Reverse primer (5’→3’)	延伸引物 Extended primer (5’→3’)
rs415006419	同义突变 Synonymous mutation	ACGTTGGATGGAAAGGGAGAAAGAGATAG	ACGTTGGATGCTTCTGCGAAGGGATGACTG	TGGGCGAGTTCTCACTCAT
rs403075278	同义突变 Synonymous mutation	ACGTTGGATGTGCAGCAGGTTCTGTACTTG	ACGTTGGATGCAGGACTAAGAAGCCCATTG	TGGGGCGTGATCGGCCC

SNPs	突变类型 Mutation type	上游引物 Forward primer(5’→3’)	下游引物 Reverse primer (5’→3’)	延伸引物 Extended primer (5’→3’)
rs415006419	同义突变 Synonymous mutation	ACGTTGGATGGAAAGGGAGAAAGAGATAG	ACGTTGGATGCTTCTGCGAAGGGATGACTG	TGGGCGAGTTCTCACTCAT
rs403075278	同义突变 Synonymous mutation	ACGTTGGATGTGCAGCAGGTTCTGTACTTG	ACGTTGGATGCAGGACTAAGAAGCCCATTG	TGGGGCGTGATCGGCCC

位点 Locus	物理位置 Physical location	突变频数Mutation frequency						群体 Group	基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
		D		XH		T			基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
				Het	Hom	Het	Hom		Het	Hom		CC	CT	TT	χ²	C	T
rs415006419	77181965	4	26	8	21	13	17	D	0	13.30	86.70	10.30	6.70	93.30
								T	0	43.30	56.70		21.70	78.30
								XH	6.50	25.80	67.70		19.40	80.60
rs403075278	77332591	8	17	13	16	18	12	D	16.70	26.70	56.70	10.80	30.00	70.00
								T	0	60.00	40.00		30.00	70.00
								XH	6.50	41.90	51.60		27.40	72.60

位点 Locus	物理位置 Physical location	突变频数Mutation frequency						群体 Group	基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
		D		XH		T			基因型频率 Genotype frequency/%				基因频率 Gene frequency/%
				Het	Hom	Het	Hom		Het	Hom		CC	CT	TT	χ²	C	T
rs415006419	77181965	4	26	8	21	13	17	D	0	13.30	86.70	10.30	6.70	93.30
								T	0	43.30	56.70		21.70	78.30
								XH	6.50	25.80	67.70		19.40	80.60
rs403075278	77332591	8	17	13	16	18	12	D	16.70	26.70	56.70	10.80	30.00	70.00
								T	0	60.00	40.00		30.00	70.00
								XH	6.50	41.90	51.60		27.40	72.60

位点	群体	He	Ho	Ne	PIC	P
Locus	Group
rs415006419	D	0.13	0.88	1.14	0.12	0.93
	T	0.42	0.58	1.72	0.33	0.14
	XH	0.34	0.66	1.51	0.28	0.32
rs403075278	D	0.42	0.58	1.72	0.33	0.14
	T	0.34	0.66	1.51	0.28	0.32
	XH	0.42	0.58	1.72	0.33	0.06

绵羊GRM1基因多态性及其与肉质性状的相关性

Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep

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位点 Locus	基因型 Genotype	宰前活重 Live weight/ kg	GR值 GR value/ cm	胴体重 Carcass weight/ kg	背膘厚 Back fat/ cm	屠宰率 Slaughter rate/%	净肉重 Net meat weight/ kg	净肉率 Net meat rate/ %	十二指肠长度 Duodenal length/ cm	质量 Weight/kg
位点 Locus	基因型 Genotype	宰前活重 Live weight/ kg	GR值 GR value/ cm	胴体重 Carcass weight/ kg	背膘厚 Back fat/ cm	屠宰率 Slaughter rate/%	净肉重 Net meat weight/ kg	净肉率 Net meat rate/ %	十二指肠长度 Duodenal length/ cm	心 Heart	肝 Liver	脾 Spleen	肺 Lung	肾 Kidney	全骨 Total bone
rs415006419	CT	26.69 ±6.20	0.75 ±0.27	12.75 ±3.90	0.37 ±0.18	47.25 ±5.25	9.64 ±3.52	35.37 ±5.87	17.00 ±3.31	0.12 ±0.02	0.37 ±0.09	0.06 ±0.03	0.35 ±0.13	0.22 ±0.11 a	3.11 ±0.53
	TT	27.68 ±5.49	0.81 ±0.31	12.38 ±2.88	0.31 ±0.13	44.65 ±4.81	9.21 ±2.64	33.13 ±5.69	16.79 ±2.61	0.13 ±0.02	0.39 ±0.10	0.05 ±0.02	0.42 ±0.15	0.14 ±0.08 b	3.16 ±0.81
rs403075278	CT	23.53 ±4.39 Bb	0.63 ±0.19	10.21 ±2.26 Bb	0.23 ±0.06 b	43.19 ±3.00	7.14 ±1.73 Bb	30.22 ±4.05 b	15.06 ±2.19 Bb	0.11± 0.02 Bb	0.33 ±0.08	0.04 ±0.02 b	0.27± 0.11 Bb	0.09± 0.07 Bb	3.07 ±0.96
	TT	29.42 ±5.32 Aa	0.86 ±0.30	13.97 ±3.36 Aa	0.37 ±0.17 a	47.32 ±5.71	10.71 ±3.02 Aa	36.16 ±5.86 a	17.82 ±2.83 Aa	0.13± 0.02 Aa	0.42 ±0.11	0.06 ±0.02 a	0.45± 0.12 Aa	0.22± 0.08 Aa	3.26 ±0.61

位点 Locus	基因型 Genotype	肌纤维直径 Muscle fiber diameter/μm	肌纤维密度 Muscle fiber density/mm^-2	pH值 pH value	剪切力 Shear force	熟肉率 Cooked meat rate/%	失水率 Water loss rate/%
rs415006419	CT	28.90±3.75	789.41±182.77	5.58±0.47	25.70±13.32	62.75±5.65	34.06±6.45
	TT	32.87±8.96	724.96±240.98	5.62±0.50	31.14±12.73	60.4±5.57	37.13±5.85
rs403075278	CT	32.22±3.66	734.56±172.22	6.08±0.45 Aa	42.67±14.53 Aa	61.17±7.26	40.76±5.06 Aa
	TT	31.05±7.92	738.68±228.79	5.44±0.40 Bb	21.92±13.21 Bb	62.75±5.64	33.44±5.27 Bb

位点 Locus	基因型 Genotype	脂肪 Fat/%	蛋白质 Protein/ %	肌酐 Creat- inine/ (μg· g^-1)	肌苷酸 Creatinine acid/ (μg· g^-1)	胆固醇 Inosinic acid/ (μg· g^-1)	铜 Cu/ (mg· kg^-1)	锌 Zn/ (mg· kg^-1)	硒 Se/ (mg· kg^-1)	钙 Ca/ (mg· kg^-1)	铁 Fe/ (mg· kg^-1)	锰 Mn/ (mg· kg^-1)	天冬氨酸 Aspartic acid/ (mg· g^-1)	苏氨酸 Threonine/ (mg· g^-1)	丝氨酸 Serine/ (mg· g^-1)
rs415006419	CT	3.46 ±1.84	20.90 ±1.34	450.34 ±98.30	1838.03 ±339.12	307.59 ±64.54	1.62 ±0.39	24.38 ±2.80	221.86 ±91.32	139.74 ±13.68	23.11 ±4.19	291.01 ±42.07	1.72 ±0.23	0.92 ±0.11	0.82 ±0.12
	TT	3.46 ±1.93	20.77 ±1.21	456.13 ±131.79	1978.06 ±350.81	313.07 ±56.14	1.42 ±0.47	25.75 ±3.83	195.03 ±82.99	142.94 ±31.95	23.25 ±4.17	285.15 ±27.30	1.86 ±0.39	0.99 ±0.19	0.86 ±0.17
rs403075278	CT	2.25 ±0.83 b	20.30 ±1.22	547.64 ±86.89	2054.80 ±292.85	290.24 ±70.96	1.51 ±0.39	26.33 ±1.81	174.92 ±20.87	126.84 ±23.80 b	24.27 ±3.90	283.74 ±18.41	1.73 ±0.42	0.94 ±0.20	0.80 ±0.18
	TT	4.07 ±1.92 a	21.22 ±1.21	437.73 ±160.29	1776.24 ±451.47	316.67 ±53.81	1.49 ±0.49	24.47 ±3.85	220.26 ±98.90	147.04 ±22.84 a	22.97 ±4.38	287.76 ±39.57	1.83 ±0.29	0.97 ±0.14	0.87 ±0.13
位点 Locus	基因型 Genotype	谷氨酸 Gluta- mate/ (mg· g^-1)	甘氨酸 Glycine/ (mg· g^-1)	丙氨酸 Alanine/ (mg· g^-1)	半胱氨酸 Cysteine/ (mg· g^-1)	缬氨酸 Valine/ (mg· g^-1)	甲硫氨酸 Methionine/ (mg· g^-1)	异亮氨酸 Isole- ucine/ (mg· g^-1)	亮氨酸 Leucine/ (mg· g^-1)	酪氨酸 Tyrosine/ (mg· g^-1)	苯丙氨酸 Phenyla- lanine/ (mg· g^-1)	赖氨酸 Lysine/ (mg· g^-1)	组氨酸 Histidine/ (mg· g^-1)	精氨酸 Argin- ine/ (mg· g^-1)	脯氨酸 Proline/ (mg· g^-1)
rs415006419	CT	2.90 ±0.37	0.83 ±0.11	1.27 ±0.23	0.03 ±0.06	1.00 ±0.17	0.49 ±0.13	0.96 ±0.13	1.68 ±0.18	0.67 ±0.14 b	1.26 ±0.21	1.95 ±0.29	0.88 ±0.20	1.16 ±0.11	1.10 ±0.22
	TT	3.17 ±0.65	0.90 ±0.17	1.30 ±0.26	0.05 ±0.08	1.13 ±0.35	0.62 ±0.35	1.08 ±0.31	1.87 ±0.42	0.83 ±0.23 a	1.32 ±0.27	2.07 ±0.37	0.90 ±0.21	1.30 ±0.24	1.02 ±0.31
rs403075278	CT	2.98 ±0.73	0.84 ±0.21	1.17 ±0.34	0.04 ±0.02	1.03 ±0.43	0.59 ±0.38	1.07 ±0.38	1.81 ±0.47	0.84 ±0.25	1.13 ±0.30 b	1.90 ±0.48	0.76 ±0.16 b	1.29 ±0.29	1.18 ±0.36
	TT	3.07 ±0.47	0.89 ±0.12	1.35 ±0.18	0.04 ±0.08	1.10 ±0.20	0.55 ±0.22	1.01 ±0.19	1.77 ±0.28	0.71 ±0.18	1.35 ±0.19 a	2.07 ±0.25	0.95 ±0.19 a	1.21 ±0.16	1.01 ±0.22

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