福建安溪茶树种质资源遗传多样性与铁观音衍生品种遗传关系

doi:10.3969/j.issn.1004-1524.20231037

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (7): 1591-1601.DOI: 10.3969/j.issn.1004-1524.20231037

福建安溪茶树种质资源遗传多样性与铁观音衍生品种遗传关系

朱艳宇¹(), 于文涛²^,^*(), 高水练³, 吕水源², 王攀¹, 靳宛旻³, 贵文静¹, 林浥¹, 叶乃兴¹^,^*()

1.福建农林大学园艺学院,福建福州 350002
2.福州海关技术中心,福建省检验检疫技术研究重点实验室,福建福州 350001
3.福建农林大学安溪茶学院,福建福州 350002

收稿日期:2023-08-31 出版日期:2024-07-25 发布日期:2024-08-05
作者简介:朱艳宇(2002—),女,河南信阳人,硕士研究生,研究方向为茶树栽培育种。E-mail: zyy8585065@163.com
通讯作者: *叶乃兴,E-mail: ynxtea@126.com;于文涛,E-mail: wtyu@foxmail.com
基金资助:
安溪茶叶重大科技创新专项(AX2021001);福建农林大学横向课题(KH220008A);福建省科技厅农业科技引导性项目(2021N0024);福建张天福茶叶发展基金会科技创新基金(FJZTF01)

The diversity of tea germplasm resources and genetic relationship of ‘Tieguanyin’-derived varieties in Anxi, Fujian, China

ZHU Yanyu¹(), YU Wentao²^,^*(), GAO Shuilian³, LYU Shuiyuan², WANG Pan¹, JIN Wanmin³, GUI Wenjing¹, LIN Yi¹, YE Naixing¹^,^*()

1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Technology Centre of Fuzhou Customs District, Fuzhou 350001, China
3. Anxi Tea College, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2023-08-31 Online:2024-07-25 Published:2024-08-05

摘要/Abstract

摘要：

为更好地保护、鉴定评价和利用安溪茶树种质资源,采用单核苷酸多态性(SNP)分子标记技术对安溪茶树种质资源的遗传多样性和铁观音衍生品种的遗传关系进行分析,并进行了主坐标分析、层次聚类树分析、种群结构分析和遗传距离分析,此外,还构建了铁观音及其衍生品种的DNA指纹图谱,并计算了其遗传相似系数。结果显示,共筛选出53个适用于鉴定安溪茶树种质资源基因分型的高多态性位点,观测杂合度平均值为0.322,期望杂合度平均值为0.281,说明安溪茶树种质具有较为丰富的遗传多样性;安溪4个乡镇产区茶树群体间的遗传距离在0.068~0.269。供试样品可分为2个组,组Ⅰ主要包括西坪、虎邱和湖上等产区的茶树种质,种质间交流频繁;组Ⅱ主要由福田乡产区的野生型地方茶树种质组成,福田乡茶树种质资源相对集中且独立。DNA指纹图谱可以精准鉴定铁观音及其衍生品种,铁观音衍生品种具有较为丰富的多样性,其中铁观音与金牡丹、紫玫瑰、新选211的遗传相似系数较大。综上,安溪县茶树种质资源多样性丰富,在铁观音衍生品种中,金牡丹、紫玫瑰和新选211与铁观音的遗传相似度更高。

关键词: 安溪, 铁观音衍生品种, 茶树, 种质资源, 遗传多样性, 单核苷酸多态性

Abstract:

In order to better protect, evaluate and utilize Anxi tea germplasm resources, the genetic diversity of Anxi tea germplasm resources and the genetic relationship of ‘Tieguanyin’-derived varieties were analyzed by single nucleotide polymorphism (SNP) molecular marker technique. Principal coordinate analysis, hierarchical clustering tree analysis, population structure analysis and genetic distance analysis were carried out.The DNA fingerprinting of ‘Tieguanyin’ and its derived varieties were also constructed, and their genetic similarity coefficients were calculated. The results showed that a total of 53 high polymorphic loci suitable for genotyping of Anxi tea germplasm resources were screened out. The average observed heterozygosity was 0.322, and the average expected heterozygosity was 0.281, indicating that Anxi tea plant germplasm resources had rich genetic diversity. The genetic distance between tea populations in the four townships of Anxi was 0.068-0.269. The tested samples could be divided into two groups. Group I mainly included tea plant germplasm resources from Xiping, Huqiu and Hushang, with frequent exchanges among germplasm resources. Group Ⅱ was mainly composed of wild-type landrace tea germplasm resources in Futian, and the tea plant germplasm resources in Futian were relatively concentrated and independent. DNA fingerprinting could accurately identify ‘Tieguanyin’ and its derived varieties. ‘Tieguanyin’-derived varieties had rich diversity, among which ‘Tieguanyin’ had a large genetic similarity coefficient with ‘Jinmudan’, ‘Zimeigui’ and ‘Xinxuan 211’. In summary, the diversity of tea plant germplasm resources in Anxi County was rich, ‘Jinmudan’, ‘Zimeigui’ and ‘Xinxuan 211’ had higher genetic similarity with ‘Tieguanyin’.

Key words: Anxi, ‘Tieguanyin’-derived variety, Camellia sinensis, germplasm resource, genetic diversity, single nucleotide polymorphism

中图分类号:

S571.1

朱艳宇, 于文涛, 高水练, 吕水源, 王攀, 靳宛旻, 贵文静, 林浥, 叶乃兴. 福建安溪茶树种质资源遗传多样性与铁观音衍生品种遗传关系[J]. 浙江农业学报, 2024, 36(7): 1591-1601.

ZHU Yanyu, YU Wentao, GAO Shuilian, LYU Shuiyuan, WANG Pan, JIN Wanmin, GUI Wenjing, LIN Yi, YE Naixing. The diversity of tea germplasm resources and genetic relationship of ‘Tieguanyin’-derived varieties in Anxi, Fujian, China[J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1591-1601.

图/表 9

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主坐标 Principal coordinate	贡献率 Contribution rate	累计贡献率 Cumulative contribution rate
第1主坐标 The first principal coordinate	41.37	41.37
第2主坐标 The second principal coordinate	9.92	51.29
第3主坐标 The third principal coordinate	6.17	57.46

主坐标 Principal coordinate	贡献率 Contribution rate	累计贡献率 Cumulative contribution rate
第1主坐标 The first principal coordinate	41.37	41.37
第2主坐标 The second principal coordinate	9.92	51.29
第3主坐标 The third principal coordinate	6.17	57.46

地区 Area	西坪 Xiping	虎邱 Huqiu	湖上 Hushang	福田 Futian
虎邱Huqiu	0.081
湖上Hushang	0.072	0.068
福田Futian	0.269	0.101	0.231
其他Other	0.047	0.039	0.032	0.170

地区 Area	西坪 Xiping	虎邱 Huqiu	湖上 Hushang	福田 Futian
虎邱Huqiu	0.081
湖上Hushang	0.072	0.068
福田Futian	0.269	0.101	0.231
其他Other	0.047	0.039	0.032	0.170

品种 Variety	铁观音 Tieguan- yin	黄观音 Huang- guanyin	金观音 Jinguan- yin	紫玫瑰 Zimeigui	金牡丹 Jinmu- dan	春桃香 Chuntaoxiang	新选205 Xinxuan 205	新选206 Xinxuan 206	新选209 Xinxuan 209	新选211 Xinxuan 211	新选212 Xinxuan 212	金玫瑰 Jinmei- gui	紫牡丹 Zimudan	春兰 Chunlan	凤圆春 Fengyuan- chun	金茗早 Jinming- zao
黄观音	0.545
Huangguanyin
金观音	0.600	0.527
Jinguanyin
紫玫瑰	0.655	0.509	0.618
Zimeigui
金牡丹	0.655	0.491	0.655	0.782
Jinmudan
春桃香	0.509	0.436	0.582	0.509	0.545
Chuntaoxiang
新选205	0.618	0.491	0.800	0.636	0.727	0.564
Xinxuan 205
新选206	0.600	0.473	0.782	0.636	0.764	0.600	0.709
Xinxuan 206
新选209	0.600	0.473	0.655	0.745	0.673	0.545	0.600	0.709
Xinxuan 209
新选211	0.691	0.491	0.618	0.782	0.655	0.509	0.582	0.709	0.782
Xinxuan 211
新选212	0.636	0.491	0.691	0.673	0.655	0.545	0.673	0.691	0.709	0.709
Xinxuan 212
金玫瑰	0.491	0.400	0.582	0.673	0.600	0.636	0.655	0.618	0.655	0.618	0.564
Jinmeigui
紫牡丹	0.509	0.673	0.509	0.545	0.655	0.491	0.491	0.509	0.509	0.509	0.455	0.455
Zimudan
春兰	0.509	0.491	0.473	0.382	0.345	0.436	0.418	0.509	0.564	0.455	0.455	0.382	0.364
Chunlan
凤圆春	0.527	0.400	0.473	0.400	0.473	0.418	0.582	0.527	0.582	0.527	0.491	0.473	0.455	0.436
Fengyuanchun
金茗早	0.400	0.418	0.491	0.455	0.473	0.527	0.418	0.527	0.418	0.418	0.491	0.455	0.418	0.436	0.345
Jinmingzao
黄玫瑰	0.509	0.636	0.636	0.600	0.564	0.436	0.582	0.527	0.600	0.618	0.564	0.491	0.673	0.418	0.582	0.455
Huangmeigui

福建安溪茶树种质资源遗传多样性与铁观音衍生品种遗传关系

The diversity of tea germplasm resources and genetic relationship of ‘Tieguanyin’-derived varieties in Anxi, Fujian, China

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