生物有机肥对温郁金根际微生物群落结构的影响

doi:10.3969/j.issn.1004-1524.20240239

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (4): 892-900.DOI: 10.3969/j.issn.1004-1524.20240239

生物有机肥对温郁金根际微生物群落结构的影响

韦新航¹^,²^,³(), 周铨¹^,²^,³, 李亚妮¹^,²^,³, 陈卫良¹^,²^,³, 毛碧增¹^,²^,³^,^*()

1.浙江大学生物技术研究所,浙江杭州 310058
2.农业农村部作物病虫分子生物学重点实验室,浙江杭州 310058
3.全省作物病虫生物学与生态调控重点实验室,浙江杭州 310058

收稿日期:2024-03-14 出版日期:2025-04-25 发布日期:2025-05-09
作者简介:韦新航(1997—),男,广西河池人,硕士研究生,主要研究方向为温郁金的病害防治和健康种苗繁育。E-mail:wxh5270@163.com
通讯作者: *毛碧增,E-mail: maobz@zju.edu.cn
基金资助:
浙江省“十四五”中药材新品种选育重大科技专项(2021C02074);农业农村部农业重大技术协同推广计划(2020XTTGZYC02)

Effect of bio-organic fertilizer on microbial community structure in rhizosphere of Curcuma wenyujin

WEI Xinhang¹^,²^,³(), ZHOU Quan¹^,²^,³, LI Yani¹^,²^,³, CHEN Weiliang¹^,²^,³, MAO Bizeng¹^,²^,³^,^*()

1. Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
2. Key Lab of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture and Rural Affairs, Hangzhou 310058, China
3. Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Hangzhou 310058, China

Received:2024-03-14 Online:2025-04-25 Published:2025-05-09

摘要/Abstract

摘要： 该研究以连续种植温郁金(Curcuma wenyujin Y. H. Chen et C. Ling)的土壤为研究对象,通过高通量测序技术,研究施用不同肥料对温郁金根际微生物群落结构的影响。结果显示:施用生物有机肥能够改善土壤微生物的α多样性,增加Burkholderia-Caballeronia-Paraburkholderia、不动杆菌属(Acidibacter)等有益菌、功能菌的丰度,降低镰刀菌属(Fusarium)、茎点霉属(Phoma)等病原真菌的丰度。结果表明,生物有机肥可以通过提高土壤有益菌丰度、抑制病原菌富集、改善土壤微生物群落结构,为植物生长提供有利条件,从而减缓连作障碍的发生。

关键词: 温郁金, 生物有机肥, 根际微生物, 高通量测序

Abstract:

In this study, the soil of continuous planting of Curcuma wenyujin Y. H. Chen et C. Ling was used as the research object. By using the high-throughput sequencing technology, the impact of various fertilizers on the rhizosphere microbial community structure of Curcuma wenyujin was explored. The results showed that the application of bio-organic fertilizer could improve the α-diversity of soil microorganisms, increase the abundance of beneficial and functional bacteria such as Burkholderia-Caballeronia-Paraburkholderia and Acidibacter, yet decrease the abundance of pathogenic fungi such as Fusarium and Phoma. The results indicated that the application of bio-organic fertilizer could improve the soil microbial community structure by increasing the abundance of probiotics and inhibiting the proliferation of pathogenic bacteria, thereby providing favorable conditions for plant growth, and ultimately aiding to mitigate the challenges associated with continuous cropping.

Key words: Curcuma wenyujin, bio-organic fertilizer, rhizosphere microorganism, high-throughput sequencing

中图分类号:

S471

韦新航, 周铨, 李亚妮, 陈卫良, 毛碧增. 生物有机肥对温郁金根际微生物群落结构的影响[J]. 浙江农业学报, 2025, 37(4): 892-900.

WEI Xinhang, ZHOU Quan, LI Yani, CHEN Weiliang, MAO Bizeng. Effect of bio-organic fertilizer on microbial community structure in rhizosphere of Curcuma wenyujin[J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 892-900.

图/表 6

图1 供试土壤样本中细菌(左)、真菌(右)运算分类单元(OTU)数量的花瓣图

Fig.1 Flower plot of operational taxonomic units (OTU) number of bacteria (left) and fungi (right) in test soil samples

表1 不同处理土壤细菌的α多样性指数

Table 1 Alpha diversity indexes of soil bacteria under treatments

处理 Treatment	Chao1指数 Chao1 index	深度测序指数 Goods coverage	香农指数 Shannon index	可观测物种数 Observed species	辛普森指数 Simpson index
CK	3 304.1±602.0 d	0.99±0.01 a	8.31±0.32 d	2 751.9±431.3 d	0.98±0.01 b
T1	4 186.9±216.3 a	0.99±0.01 a	9.71±0.19 a	3 677.2±169.2 a	1.00±0.01 a
T2	4 330.5±387.3 a	0.99±0.01 a	9.23±0.59 b	3 714.2±237.0 a	0.99±0.01 a
T3	3 961.8±96.4 b	0.99±0.01 a	8.94±0.08 b	3 283.0±15.7 b	0.99±0.01 a
T4	3 611.3±58.8 c	0.99±0.01 a	8.61±0.05 c	2 984.1±89.6 c	0.97±0.01 c

表2 不同处理土壤真菌的α多样性指数

Table 2 Alpha diversity indexes of soil fungi under treatments

处理 Treatment	Chao1指数 Chao1 index	深度测序指数 Goods coverage	香农指数 Shannon index	可观测物种数 Observed species	辛普森指数 Simpson index
CK	554.0±35.6 c	1.00 a	5.16±0.14 c	502.9±42.5 c	0.92±0.01 b
T1	702.5±107.6 a	1.00 a	5.77±0.43 b	620.9±64.4 a	0.95±0.03 a
T2	706.0±64.2 a	1.00 a	5.64±0.25 b	598.1±75.2 b	0.95±0.02 a
T3	631.1±34.6 b	1.00 a	4.85±0.15 c	557.7±31.3 b	0.83±0.09 c
T4	630.1±76.2 b	1.00 a	6.04±0.78 a	587.0±46.6 b	0.95±0.04 a

图2 不同处理土壤细菌(左)、真菌(右)群落的主成分分析(PCA) PC1,第1主成分;PC2,第2主成分。

Fig.2 Principle component analysis (PCA) of soil bacteria (left) and fungi (right) community PC1, Principle component 1; PC2, Principle component 2.

图3 门水平上温郁金根际土壤的细菌(左)、真菌(右)群落结构组成

Fig.3 Composition of bacteria (left) and fungi (right) community in rhizosphere soil of Curcuma wenyujin

图4 属水平上温郁金根际土壤的细菌(左)、真菌(右)群落结构组成

Fig.4 Composition of bacteria (left) and fungi (right) community in rhizosphere soil of Curcuma wenyujin

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生物有机肥对温郁金根际微生物群落结构的影响

Effect of bio-organic fertilizer on microbial community structure in rhizosphere of Curcuma wenyujin

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