›› 2009, Vol. 21 ›› Issue (3): 0-233.

• 论文 •    

石斛叶片蛋白质双向电泳技术体系的建立及优化

董 婧,应奇才,王慧中*   

  1. 杭州师范大学生物化学与分子生物学杭州市重点实验室,浙江杭州310036
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25

Establishment and optimization of two-dimensional electrophoresis for proteomic analysis of dendrobium

DONG Jing;YING Qi-cai;WANG Hui—zhong*   

  1. Key Laboratory of Molecular Biology and Biochemistry of Hangzhou, Hangzhou Normal university,Hangzhou 310036,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

摘要: 以石斛叶片为材料,建立了一套适用于石斛蛋白质组分析的双向电泳体系,探讨了石斛叶片蛋白的提取、蛋白定量以及双向电泳的一些关键技术。结果表明,通过三氯乙酸(TCA)法提取、高压等电聚焦和两步平衡等方法可以获得稳定、清晰的双向电泳图谱,获得了可分辨蛋白质斑点数约613个。

关键词: 双向电泳, 蛋白质组, 石斛

Abstract: A 2-D(two-dimensional electrophoresis)protocol suitable for protein analysis in dendrobium leaf was set up.The protein extraction and protein excretion.as well as some key technologies of 2-D electrophoresis were studied.The results
showed that stable and clear 2D maps with about 613 protein spots could be obtained through the methods of extraction with trichloroacetic acid,isoelectric focusing under high-voltage and two-step equilibrium.

Key words: two-dimensional electrophoresis, proteome, Dendrobium orchids