浙江农业学报

• 动物科学 • 上一篇    下一篇

二花脸猪繁殖性状候选基因的多态性分析

  

  1. (1 江苏省农业科学院 畜牧研究所,江苏 南京 210014;2 江苏省常熟市畜牧兽医站,江苏 常熟 215500)
  • 出版日期:2015-09-25 发布日期:2015-09-23

Genetic polymorphism of reproductive candidate gene in Erhualian pigs#br#

  1. (1 Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2 Animal Husbandry and Veterinary Station of Changshu, Changshu 210036, China)
  • Online:2015-09-25 Published:2015-09-23

摘要: 采用PCR及PCR\|RFLP技术对FSHβ,ESR和PRLR基因在二花脸猪群体内的多态性及对繁殖性状的影响进行了检测与分析。结果表明:108头二花脸母猪中检测到FSHβ基因的AA和AB型,ESR和PRLR基因的AA,AB和BB三种基因型。FSHβ基因AA型初产和经产母猪的总产仔数(TNB)和产活仔数(NBA)均高于AB型(P<005或P<001);ESR基因BB型初产及经产TNB和NBA均高于AB型(P<005)和AA型(P<001);PRLR基因AA型和AB型初产和经产TNB和NBA均高于BB型(P<001)。FSHβ基因AB型初产和经产初生个体重(BW)和断奶个体重(WW)均高于AA型(P>005);ESR基因AA型初产和经产BW高于AB和BB型,WW高于BB型(P<005);PRLR基因BB型初产和经产仔猪的WW均显著高于AA型(P<005)。FSHβ,PRLR和ESR基因对二花脸猪繁殖性状有一定影响,可作为二花脸猪繁殖性状辅助选择的标记作进一步研究。

关键词: 二花脸猪, FSH&beta, 基因, ESR基因, PRLR基因

Abstract: The follicle\|stimulating hormone β submit gene (FSHβ), estrogen receptor gene (ESR) and prolactin receptor gene (PRLR) of Erhualian pigs were investigated using PCR and PCR\|RFLP. The results showed that only AA and AB genotypes were found for FSHβ gene, and AA, AB and BB genotypes were found for ESR and PRLR genes. Pigs with AA genotype for FSHβ had more total number born (TNB) and number born alive (NBA) than AB ones (P<005 or P<001). Pigs with BB genotype for ESR had more TNB and NBA than AB (P<005) and AA ones (P<001). Pigs with AA and AB genotypes for PRLR gene had more TNB and NBA than BB ones (P<001). In the aspect of birth weight (BW) and weaning weight (WW) traits, pigs with AB genotype for FSHβ had higher BW and WW than AA ones (P>005) in the first and multiparity. Pigs with AA genotype for ESR had higher BW than AB and BB ones (P<005), and had higher WW than BB ones (P<005) in the first and multiparity. Pigs with BB genotype for PRLR had higher WW than AA ones (P<005) in the first and multiparity. FSHβ, ESR and PRLR genes had certain effects on the reproductive traits of Erhualian pigs, so genetic polymorphism of these genes could be used as important genetic markers to study reproductive traits of Erhualian pigs.

Key words:  Erhualian pig, FSH&beta, gene; , ESR gene, PRLR gene