浙江农业学报

• 动物科学 • 上一篇    下一篇

万载黑兔微卫星标记的遗传多样性分析

  

  1. (江西省农业科学院 畜牧兽医研究所,江西 南昌 330200)
  • 出版日期:2016-04-25 发布日期:2016-04-27

Analysis on genetic diversity of Wanzai black rabbit based on microsatellite markers

  1. (Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China)
  • Online:2016-04-25 Published:2016-04-27

摘要: 对万载黑兔、皖系长毛兔和比利时兔进行遗传多样性检测。利用13个微卫星位点,进行哈代温伯格平衡(HardyWeinberg equilibrium,HWE)检验,统计3个种群的基因频率、观察杂合度(Ho)、期望杂合度(He)、F值和遗传距离。研究结果表明:万载黑兔、比利时兔、皖系长毛兔各存在3个不平衡位点,说明群体遗传多样性较丰富。3个群体多态信息含量(PIC)平均值分别为0.35,0.48,0.31。3个群体的He平均值分别为0.40,0.54,0.37;Ho平均值分别为0.39,0.51,0.35。群体间遗传分化系数(Fst)平均为0.120 1,万载黑兔与比利时兔的遗传距离最近,为0.145 2;皖系长毛兔与万载黑兔的遗传距离最远,为0.200 0。

关键词: 万载黑兔, 皖系长毛兔, 比利时兔, 微卫星标记, 遗传多样性

Abstract:  Genetic diversities of Wanzai black rabbit, Wanxi Angora rabbit and Belgian rabbit were studied using 13 microsatellite markers. The genetic diversity was disclosed by calculating the allele frequency, heterozygosity (Ho), expected heterozygosity (He), F value and genetic distance. It was shown that there were 3 imbalance locus in each of the above mentioned rabbit populations, indicating a rich genetic diversity. The average polymorphism information contents (PIC) of Wanzai black rabbit, Wanxi Angora rabbit and Belgian rabbit were 0.35, 0.48, 0.31, respectively. The average He were 0.40, 0.54, 0.37, respectively, and the average Ho were 0.39, 0.51, 0.35, respectively. The average genetic differentiation coefficient (Fst) value among populations was 0.120 1. The observed closest genetic distance was 0.145 2 between Wanzai black rabbit and Belgian rabbit, and the observed farthest genetic distance was 0.200 0 between Wanzai black rabbit and Wanxi Angora rabbit.

Key words: Wanzai black rabbit, Wanxi Angora rabbit, Belgian rabbit, microsatellite markers, genetic diversity