草莓微生物污染分析及革兰氏阴性细菌和霉菌鉴定

doi:10.3969/j.issn.1004-1524.2017.01.20

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 144-150.DOI: 10.3969/j.issn.1004-1524.2017.01.20

草莓微生物污染分析及革兰氏阴性细菌和霉菌鉴定

何祥祥^{1, 2, 3}, 肖英平^{2, 3}, 吴声敢^{2, 3}, 赵学平^{2, 3}, 夏效东¹, 杨华^{2, 3, *}

1.西北农林科技大学食品科学与工程学院,陕西杨凌 712100;
2.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021;
3.浙江省植物有害生物防控省部共建国家重点实验室培育基地,浙江杭州 310021

收稿日期:2016-07-13 出版日期:2017-01-15 发布日期:2017-02-23
通讯作者: 杨华,E-mail:yanghua806@hotmail.com
作者简介:何祥祥(1991—),男,河南洛阳人,硕士研究生,研究方向为食品科学。E-mail:xianghe2009@126.com
基金资助:
浙江省特色农产品全产业链安全风险管控项目基金(ZJNY2016001); 公益性行业(农业)科研专项(201303088)

Analysis of microbial contamination and identification of gram negative bacteria and mold in strawberry

HE Xiangxiang^{1, 2, 3}, XIAO Yingping^{2, 3}, WU Shenggan^{2, 3}, ZHAO Xueping^{2, 3}, XIA Xiaodong¹, YANG Hua^{2, 3, *}

1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China;;
2. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-07-13 Online:2017-01-15 Published:2017-02-23

摘要/Abstract

摘要： 为评估草莓的微生物污染情况,在2016年3—4月份对浙江地区基地温棚和市售草莓进行随机抽样,分析草莓中菌落总数、大肠菌群、霉菌,并运用全自动微生物分析系统(VITEK)和分子生物学技术,分别对分离的革兰氏阴性细菌和霉菌进行了鉴定。结果表明:温棚草莓菌落总数为120~48 600 cfu·g^-1,大肠菌群为3~1 100 MPN·g^-1,霉菌为0~2 900 cfu·g^-1;市场草莓菌落总数为1 900~36 900 cfu·g^-1,大肠菌群为15~1 100 MPN·g^-1,霉菌为200~6 100 cfu·g^-1;同时鉴定出大肠埃希菌(Escherichia coli)、阴沟肠杆菌(Enterobacter cloacae)、河生肠杆菌(Enterobacter.amnigenus)、植生拉乌尔菌(Raoultella planticola)等19株革兰氏阴性细菌和毛霉属(Mucor)、镰刀菌属(Fusarium)等5株霉菌,所检出的革兰氏阴性细菌和霉菌多数具有较强的致病性。研究结果为草莓微生物数量的控制和开发杀菌抑菌技术提供了参考。

关键词: 草莓, 微生物, 革兰氏阴性细菌, 霉菌

Abstract: In order to evaluate the status of microbial contamination in strawberry, hygienical microbiology was examined from strawberry which sampled in greenhouse and market of Zhejiang Province during March to April in 2016 year according to the food hygiene standard of People Republic of China. Then bacteria counting, enumeration of coliforms and mold detection were carried out. The bacteria and mold which were isolated from the samples were identified by Vitek Auto Microbic System and Molecular biological methods, respectively. The results indicated that the number of bacteria, coliforms and mold of strawberry in greenhouse ranged from 120 to 48 600 cfu·g^-1, from 3 to~1 100 MPN·g^-1, from 0 to 2 900 cfu·g^-1, respectively. And the number of bacteria, coliforms and mold of strawberry in market ranged from 1 900 to 36 900 cfu·g^-1, from 15 to 1 100 MPN·g^-1, from 200 to 6 100 cfu·g^-1, respectively. Meanwhile, 19 gram negative bacteria species such as Escherichia coli, Enterobacter cloacae, Enterobacter amnigenus, Raoultella planticola and 5 mold species such as Mucor and Fusarium were identified. Most of the identified bacteria and mold had serious pathogenicity. The experimental results would be helpful to control the number of microorganisms and develop new sterile technology for strawberry.

Key words: strawberry, microorganism, gram negative bacteria, mold

中图分类号:

S436.68

何祥祥, 肖英平, 吴声敢, 赵学平, 夏效东, 杨华. 草莓微生物污染分析及革兰氏阴性细菌和霉菌鉴定[J]. 浙江农业学报, 2017, 29(1): 144-150.

HE Xiangxiang, XIAO Yingping, WU Shenggan, ZHAO Xueping, XIA Xiaodong, YANG Hua. Analysis of microbial contamination and identification of gram negative bacteria and mold in strawberry[J]. , 2017, 29(1): 144-150.

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草莓微生物污染分析及革兰氏阴性细菌和霉菌鉴定

Analysis of microbial contamination and identification of gram negative bacteria and mold in strawberry

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