聚类分析法研究不同产地花生壳高效液相色谱指纹图谱

doi:10.3969/j.issn.1004-1524.2018.12.20

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (12): 2137-2143.DOI: 10.3969/j.issn.1004-1524.2018.12.20

聚类分析法研究不同产地花生壳高效液相色谱指纹图谱

赵亮, 陈月, 方应国, 饶桂维^*

浙江树人大学生物与环境工程学院,浙江杭州 310015

收稿日期:2018-03-09 出版日期:2018-12-25 发布日期:2018-12-28
通讯作者: 饶桂维,E-mail: xiankelaifeng@163.com
作者简介:赵亮(1997—),女,江苏盐城人,本科生,研究方向为食品检测。E-mail: 3294572650@qq.com
基金资助:
浙江树人大学中青年学术团队资助项目(20180406)

HPLC fingerprint of peanut hulls from different habitats by cluster analysis

ZHAO Liang, CHEN Yue, FANG Yingguo, RAO Guiwei^*

College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China

Received:2018-03-09 Online:2018-12-25 Published:2018-12-28

摘要/Abstract

摘要： 对8个产地的花生壳样品进行了HPLC指纹图谱研究,优化了样品提取条件和色谱测定条件,并进行了聚类分析。优化的色谱条件为:WondaCract ODS-2 C₁₈(4.6 mm×150 mm,5 μm)色谱柱,以乙腈-0.5%磷酸溶液为流动相进行梯度洗脱,流速1 mL·min^-1,柱温35 ℃,波长290 nm。建立8个产地花生壳样品的HPLC指纹图谱,标示出14个稳定的共有峰,共有模式的相似度除新昌小京生外,皆大于0.9。对共有峰平均相对峰面积的聚类分析表明,8种花生壳可分为两大类。

关键词: 花生壳, HPLC指纹图谱, 聚类分析

Abstract: The HPLC fingerprints of peanut shell samples from 8 habitats were studied and cluster analysis was carried out. The conditions of sample extraction and chromatographic were optimized. The optimized chromatographic conditions were WondaCract ODS-2 C₁₈ (4.6 mm×150 mm, 5 μm) column and gradient elution with acetonitrile-0.5% phosphate solution as mobile phase. The flow rate was 1 mL·min^-1, the column temperature was 35 ℃, and the wavelength was 290 nm. The HPLC fingerprints of peanut shell samples from 8 regions were established, indicating 14 stable common peaks. The similarity of common patterns was larger than 0.9 except Xinchang Xiaojingsheng. The cluster analysis of the average peak area of the common peaks showed that the 8 peanut shells could be divided into two categories.

Key words: peanut shell, HPLC fingerprint, cluster analysis

中图分类号:

赵亮, 陈月, 方应国, 饶桂维. 聚类分析法研究不同产地花生壳高效液相色谱指纹图谱[J]. 浙江农业学报, 2018, 30(12): 2137-2143.

ZHAO Liang, CHEN Yue, FANG Yingguo, RAO Guiwei. HPLC fingerprint of peanut hulls from different habitats by cluster analysis[J]. , 2018, 30(12): 2137-2143.

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聚类分析法研究不同产地花生壳高效液相色谱指纹图谱

HPLC fingerprint of peanut hulls from different habitats by cluster analysis

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