大通牦牛TLR2基因SNP位点筛选及生物信息学分析

doi:10.3969/j.issn.1004-1524.2019.08.08

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (8): 1280-1285.DOI: 10.3969/j.issn.1004-1524.2019.08.08

大通牦牛TLR2基因SNP位点筛选及生物信息学分析

彭帅, 陈朗, 郑天宇, 陆会宁, 张丽, 刘丽霞^*

西北民族大学生命科学与工程学院,甘肃兰州 730030

收稿日期:2018-12-29 出版日期:2019-08-25 发布日期:2019-08-30
通讯作者: 刘丽霞,E-mail: skllx@xbmu.edu.cn
作者简介:彭帅(1998-),男,湖南长沙人,本科生,研究方向为生物化学与分子生物学。E-mail:1609409157@qq.com
基金资助:
西北民族大学中央高校基本科研业务费资金(31920170029,Y18066); 西北民族大学本科教学建设项目(2018ZDSFJXTD-02)

SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak

PENG Shuai, CHEN Lang, ZHENG Tianyu, LU Huining, ZHANG Li, LIU Lixia^*

College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China

Received:2018-12-29 Online:2019-08-25 Published:2019-08-30

摘要/Abstract

摘要： 采用DNA混合池扩增后直接测序的方法,对大通牦牛TLR2基因CDS区的SNP位点进行筛选,估算各SNP位点等位基因频率,并通过生物信息学软件预测突变对TLR2 mRNA和蛋白质结构的影响。结果表明,大通牦牛TLR2基因CDS区存在2个SNP位点,分别为G⁶⁷⁷A和G¹⁵⁸⁷A,其中G⁶⁷⁷A为错义突变,导致半胱氨酸(Cys)转变为酪氨酸(Tyr)。生物信息学分析结果表明,G⁶⁷⁷A和G¹⁵⁸⁷A位点均降低了mRNA二级结构的稳定性,且蛋白二、三级结构组成也发生了改变。

关键词: 大通牦牛, TLR2基因, SNP位点, 生物信息学

Abstract: The SNP sites of the coding region of TLR2 gene in Datong yak was screened by DNA mixed pool amplification and direct sequencing, and the allele frequency of each SNP site was estimated, and the effect of mutation to TLR2 mRNA and protein structure was predicted by the bioinformatics software. The results showed that there were two SNP sites in the coding region of Datong yak TLR2 gene, G⁶⁷⁷A and G¹⁵⁸⁷A respectively, of which G⁶⁷⁷A was missense mutation, resulting in the transformation of cysteine (Cys) to tyrosine (Tyr). The results of bioinformatics analysis showed that both G⁶⁷⁷A and G¹⁵⁸⁷A sites reduced the stability of the secondary structure of the RNA, and the secondary and tertiary structure of the protein also changed.

Key words: Datong yak, TLR2 gene, SNP site, bioinformatics

中图分类号:

S823.8⁺5

彭帅, 陈朗, 郑天宇, 陆会宁, 张丽, 刘丽霞. 大通牦牛TLR2基因SNP位点筛选及生物信息学分析[J]. 浙江农业学报, 2019, 31(8): 1280-1285.

PENG Shuai, CHEN Lang, ZHENG Tianyu, LU Huining, ZHANG Li, LIU Lixia. SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak[J]. , 2019, 31(8): 1280-1285.

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大通牦牛TLR2基因SNP位点筛选及生物信息学分析

SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak

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