外源钙对荷花适应盐胁迫的影响

doi:10.3969/j.issn.1004-1524.2020.02.08

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (2): 243-252.DOI: 10.3969/j.issn.1004-1524.2020.02.08

外源钙对荷花适应盐胁迫的影响

刘艺平, 苏少文, 张琳, 刘莹, 黄志远, 贺丹, 孔德政^*

河南农业大学林学院,河南郑州 450000

收稿日期:2019-10-22 出版日期:2020-02-25 发布日期:2020-03-13
通讯作者: *孔德政,E-mail:kzxy303@163.com
作者简介:刘艺平(1977—),女,河南温县人,博士,副教授,研究方向为风景园林植物资源应用。E-mail:lyp_163@163.com
基金资助:
国家自然科学基金(31600568); 河南省高等学校重点科研项目(16B220002); 河南农业大学科技创新基金(KJCX2017C01)

Effect of exogenous calcium on lotus adaptation to salt stress

LIU Yiping, SU Shaowen, ZHANG Lin, LIU Ying, HUANG Zhiyuan, HE Dan, KONG Dezheng^*

College of Forestry, Henan Agricultural University, Zhengzhou 450000, China

Received:2019-10-22 Online:2020-02-25 Published:2020-03-13

摘要/Abstract

摘要： 研究盐胁迫条件下外源钙对荷花生长、生理生化指标和Ca²⁺相关基因表达量变化的影响,可以为深入研究荷花耐盐分子机理和利用分子辅助育种途径改良荷花资源的耐盐性提供理论指导。以凤舞、粉美人、红楼、鲜娇、春红和水晶粉七号为材料进行盆栽试验,设置5个盐分梯度,并采用盐害指数筛选盐敏感品种和盐抗性品种。外源钙处理后,研究其对盐胁迫(100 mmol·L^-1)下荷花生长过程和NnCIPK6基因表达量的影响。150、200 mmol·L^-1NaCl处理对荷花的生长有明显的抑制作用。盐胁迫下,随着外源钙施用量的增加,各项生理指标基本呈现先增后降的趋势。盐胁迫下荷花叶绿素含量均明显降低,盐敏感品种粉美人的降幅更为明显。在10、15 mmol·L^-1 CaCl₂处理下,超氧化物歧化酶(SOD)活性上升,活性氧清除能力提高,可溶性物质脯氨酸积累量增加,表明适宜浓度的外源钙能够缓解盐害。盐胁迫下粉美人、水晶粉七号的NnCIPK6基因表达量均呈明显升高趋势,以盐敏感品种粉美人的变化幅度最为显著。10 mmol·L^-1 CaCl₂处理对盐胁迫缓解效果较好,有利于改善荷花生长发育状况。

关键词: 荷花, 外源钙, 盐胁迫, 叶绿素, 超氧化物歧化酶, NnCIPK6基因

Abstract: Effects of exogenous calcium on the growth of lotus flower, physiological and biochemical indices and expression of Ca²⁺-related genes under salt stress were studied, which could provide theoretical guidance for further study on molecular mechanism of salt tolerance in lotus and the improvement of salt tolerance of lotus resources by molecular-assisted breeding. Pot experiment was carried out with Fengwu, Fenmeiren, Honglou, Xianjiao, Chunhong and Shuijingfen No. 7, five salt concentration gradients were set, and salt damage index was used to screen salt sensitive varieties and salt-resistant varieties. After treatment with exogenous calcium, effects of calcium on the physiological process of lotus growth and the expression of NnCIPK6 gene under salt stress (100 mmol·L^-1)were studied. The results showed that 150 and 200 mmol·L^-1 NaCl significantly inhibited the growth of lotus. Under salt stress, physiological indices of lotus seedlings showed a trend of rising first and then decreasing with the increase of Ca²⁺ amount, and chlorophyll content of lotus was significantly reduced, and the decline of salt-sensitive variety Fenmeiren was more obvious. Under the treatment of 10,15 mmol·L^-1CaCl₂, antioxidant enzyme superoxide dismutase(SOD) activity and active oxygen scavenging ability were increased, and soluble substance proline accumulation increased,which indicated that the appropriate concentration of exogenous calcium could alleviate lotus salt damage. Under the salt stress treatment, the expression levels of NnCIPK6 genes in Fanmeiren and Jingfenfen No. 7 showed a significant increase trend, and the salt-sensitive variety Fanmeiren showed the most significant change.The variation of the sensitive variety Fenmeiren was the most significant. Treatment with 10 mmol·L^-1 CaCl₂ had a better effect on salt stress, which was beneficial to improve the growth and development of lotus.

Key words: lotus, exogenous calcium, salt damage, chlorophyll, superoxide dismutase, NnCIPK6 gene

中图分类号:

刘艺平, 苏少文, 张琳, 刘莹, 黄志远, 贺丹, 孔德政. 外源钙对荷花适应盐胁迫的影响[J]. 浙江农业学报, 2020, 32(2): 243-252.

LIU Yiping, SU Shaowen, ZHANG Lin, LIU Ying, HUANG Zhiyuan, HE Dan, KONG Dezheng. Effect of exogenous calcium on lotus adaptation to salt stress[J]. , 2020, 32(2): 243-252.

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外源钙对荷花适应盐胁迫的影响

Effect of exogenous calcium on lotus adaptation to salt stress

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