盐胁迫与供磷水平对柳枝稷苗期生理特性的影响

doi:10.3969/j.issn.1004-1524.2022.06.11

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (6): 1205-1216.DOI: 10.3969/j.issn.1004-1524.2022.06.11

盐胁迫与供磷水平对柳枝稷苗期生理特性的影响

麻仲花^a(), 吴娜^a, 陈娟^a, 赵匆^a, 闫承宏^a, 刘吉利^b^,^c^,^*()

a.宁夏大学农学院,宁夏银川 750021
b.宁夏大学生态环境学院,宁夏银川 750021
c.宁夏大学西北土地退化与生态恢复国家重点实验室培育基地,宁夏银川 750021

收稿日期:2021-02-01 出版日期:2022-06-25 发布日期:2022-06-30
作者简介:*刘吉利,E-mail: tim11082003@163.com
麻仲花（1995—）,女,甘肃临洮人,硕士研究生,主要研究方向为作物栽培与耕作学。E-mail: 2504075822@qq.com
通讯作者: 刘吉利
基金资助:
国家自然科学基金(31860344)

Effects of salt stress and phosphorus supply on physiological characteristics of switchgrass seedlings

MA Zhonghua^a(), WU Na^a, CHEN Juan^a, ZHAO Cong^a, YAN Chenghong^a, LIU Jili^b^,^c^,^*()

a. College of Agriculture, Ningxia University, Yinchuan 750021, China
b. College of Ecology and Environment, Ningxia University, Yinchuan 750021, China
c. State Key Laboratory Breeding Base of Northwest Land Degradation and Ecological Restoration, Ningxia University, Yinchuan 750021, China

Received:2021-02-01 Online:2022-06-25 Published:2022-06-30
Contact: LIU Jili

摘要/Abstract

摘要：

为了明确不同柳枝稷品种对盐胁迫和供磷水平两个因素的生理响应特征,本研究采用水培试验,设置2个盐胁迫水平（A1盐胁迫、A2非盐胁迫）和4个供磷水平（B1无磷、B2低磷、B3高磷、B4全磷）,分析了盐胁迫和供磷水平对Alamo加倍体、Alamo和Pathfinder 3个柳枝稷品种苗期生理特性的影响规律。结果表明：Alamo加倍体、Alamo和Pathfinder在盐胁迫条件下可溶性糖含量随着供磷水平的增加而降低,全磷处理下较无磷处理分别降低40.80%、26.47%、13.82%。盐胁迫下3个柳枝稷品种丙二醛含量随着供磷水平的提高均在高磷处理下达到最大值。3个柳枝稷品种在盐胁迫下脯氨酸含量随着供磷水平增加而增加,全磷处理较无磷处理分别升高 136.79%、193.85%、61.09%。盐胁迫下Alamo 加倍体和Alamo的过氧化物酶（POD）活性随着供磷水平下的提高均在全磷处理下达到最大值;而Pathfinder的POD活性在低磷处理下达到最大值。盐胁迫下Alamo 加倍体的超氧化物歧化酶（SOD）活性随着供磷水平提高在高磷处理下达到最大值;盐胁迫下Alamo和Pathfinder的SOD活性随着供磷水平的提高均在低磷处理下达到最大值。3个柳枝稷品种在盐胁迫条件下过氧化氢酶（CAT）活性随着供磷水平增加而降低,全磷处理较无磷处理著降低 65.81%、37.41%、90.66%。通过对3个柳枝稷品种综合生理抗逆性分析得出Pathfinder品种在各处理下综合值之和最高,表明Pathfinder苗期抗盐胁迫与低磷胁迫能力最强,适宜在盐碱地种植。

关键词: 盐胁迫, 供磷水平, 生理特性, 主成分分析

Abstract:

In order to clarify the physiological response characteristics of different switchgrass varieties to the two factors of salt stress and phosphorus supply level, this study adopted a hydroponic experiment with 2 salt stress levels (A1 salt stress, A2 non-salt stress) and 4 phosphorus supply levels (B1 no phosphorus, B2 low phosphorus, B3 high phosphorus, B4 full phosphorus) were set, the effects of salt stress and phosphorus supply levels on The results showed that the soluble sugar content of Alamo syndiploid, Alamo and Pathfinder decreased with the increase of the phosphorus supply level under salt stress conditions, and the total phosphorus treatment decreased by 40.80%, 26.47%, and 13.82% compared with the non-phosphorus treatment. The malondialdehyde (MDA) content of the three switchgrass varieties under salt stress reached the maximum under the high phosphorus treatment with the increase of the phosphorus supply level. The proline content of the three switchgrass varieties increased with the increase of the phosphorus supply level under salt stress, and the total phosphorus treatment increased by 136.79%, 193.85%, and 61.09% respectively compared with the non-phosphorus treatment. Under salt stress, the peroxidase (POD) activity of Alamo syndiploid and Alamo reached the maximum under the full phosphorus treatment with the increase of the phosphorus supply level; while POD activity of Pathfinder reached the maximum under the low phosphorus treatment. The superoxide dismutase (SOD) activity of Alamo syndiploid under salt stress reached the maximum under high phosphorus treatment with the increase of phosphorus supply level; the SOD activity of Alamo and Pathfinder under salt stress reached the maximum under low phosphorus treatment with the increase of phosphorus supply level value. The CAT activity of the three switchgrass varieties decreased with the increase of the phosphorus supply level under the salt stress condition. Compared with the non-phosphorus treatment, the catalase (CAT) activity decreased by 65.81%, 37.41%, and 90.66%. According to the comprehensive physiological stress resistance analysis of 3 switchgrass varieties, it was concluded that Pathfinder variety has the highest sum of comprehensive values under each treatment, indicating that Pathfinder has the strongest resistance to salt stress and low phosphorus stress at seedling stage and is suitable for planting in saline-alkali land.

Key words: salt stress, phosphorus supply level, physiological characteristics, principal component analysis

中图分类号:

麻仲花, 吴娜, 陈娟, 赵匆, 闫承宏, 刘吉利. 盐胁迫与供磷水平对柳枝稷苗期生理特性的影响[J]. 浙江农业学报, 2022, 34(6): 1205-1216.

MA Zhonghua, WU Na, CHEN Juan, ZHAO Cong, YAN Chenghong, LIU Jili. Effects of salt stress and phosphorus supply on physiological characteristics of switchgrass seedlings[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1205-1216.

图/表 17

参考文献 31

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品种	生态类型	染色体倍数	原产地
Varieties	Ecotype	Ploidy level	Origin
Alamo	低地Lowland	四倍体Tetraploid	得克萨斯州南部Southern Texas 28°N
Alamo加倍体Alamo syndiploid	低地Lowland	八倍体 Octoploid	Alamo通过秋水仙素人工加倍Alamo doubled artificially with colchicine
Pathfinder	高地Upland	八倍体 Octoploid	内布拉斯加州堪萨斯州 Nebraska/Kansas 40°N

品种	生态类型	染色体倍数	原产地
Varieties	Ecotype	Ploidy level	Origin
Alamo	低地Lowland	四倍体Tetraploid	得克萨斯州南部Southern Texas 28°N
Alamo加倍体Alamo syndiploid	低地Lowland	八倍体 Octoploid	Alamo通过秋水仙素人工加倍Alamo doubled artificially with colchicine
Pathfinder	高地Upland	八倍体 Octoploid	内布拉斯加州堪萨斯州 Nebraska/Kansas 40°N

营养成分 Component	母液浓度Initial solution concentration/ (mol·L^-1)	溶液浓度 Solution concentration / （mmol·L^-1）	供磷水平Phosphorus level
营养成分 Component	母液浓度Initial solution concentration/ (mol·L^-1)	溶液浓度 Solution concentration / （mmol·L^-1）	P0/mL	-P/mL	+P/mL	P/mL
Ca(NO₃)₂·4H₂O	5	5.0	1	1	1	1
KNO₃	2.5	5.0	2	2	2	2
MgSO₄·7H₂O	2.5	2.5	1	1	1	1
KH₂PO₄	1	2.0	_	0.005	0.2	2
NaH₂PO₄	_	2.0	_	_	_	_
NaNO₃	_	5.0	_	_	_	_
MgCl₂	_	2.5	_	_	_	_
NaSO₄	_	2.5	_	_	_	_
CaCl₂	_	5.0	_	_	_	_
KCL	2.5	5.0	0.8	0.798	0.72	_
EDTA-FeNa	0.2	0.02	0.1	0.1	0.1	0.1
MnSO₄·H₂O	0.672 2	0.006 722	0.1	0.1	0.1	0.1
CuSO₄·5H₂O	0.003 16	0.000 316	0.1	0.1	0.1	0.1

营养成分 Component	母液浓度Initial solution concentration/ (mol·L^-1)	溶液浓度 Solution concentration / （mmol·L^-1）	供磷水平Phosphorus level
营养成分 Component	母液浓度Initial solution concentration/ (mol·L^-1)	溶液浓度 Solution concentration / （mmol·L^-1）	P0/mL	-P/mL	+P/mL	P/mL
Ca(NO₃)₂·4H₂O	5	5.0	1	1	1	1
KNO₃	2.5	5.0	2	2	2	2
MgSO₄·7H₂O	2.5	2.5	1	1	1	1
KH₂PO₄	1	2.0	_	0.005	0.2	2
NaH₂PO₄	_	2.0	_	_	_	_
NaNO₃	_	5.0	_	_	_	_
MgCl₂	_	2.5	_	_	_	_
NaSO₄	_	2.5	_	_	_	_
CaCl₂	_	5.0	_	_	_	_
KCL	2.5	5.0	0.8	0.798	0.72	_
EDTA-FeNa	0.2	0.02	0.1	0.1	0.1	0.1
MnSO₄·H₂O	0.672 2	0.006 722	0.1	0.1	0.1	0.1
CuSO₄·5H₂O	0.003 16	0.000 316	0.1	0.1	0.1	0.1

变异来源Source	Alamo加倍体Alamo syndiploid	Alamo	Pathfinder
A因素 A Level	*	NS	*
B因素 B Level	NS	NS	NS
A×B	*	*	*

盐胁迫与供磷水平对柳枝稷苗期生理特性的影响

Effects of salt stress and phosphorus supply on physiological characteristics of switchgrass seedlings

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主成分 Principal component	初始特征值 Initial eigenvalues			提取的主成分 The extracted principal component
主成分 Principal component	特征值 Eigenvalues	贡献率 Contribution rate/%	累积贡献率Cumulative contribution rate /%	特征值 Eigenvalues	贡献率 Contribution rate/%	累积贡献率Cumulative contribution rate/%
1	2.27	37.88	37.88	2.27	33.88	37.88
2	1.43	23.80	61.68	1.43	23.80	61.68
3	1.21	20.16	81.84	1.21	20.16	81.84
4	0.57	9.57	91.41
5	0.37	4.65	97.53
6	0.15	2.47	100.00

指标Index	PC1	PC2	PC3
SS	0.52	0.41	0.14
MDA	0.49	0.21	-0.35
PRO	0.50	0	0.39
POD	-0.13	0.03	0.84
SOD	0.44	-0.44	0.04
CAT	-0.15	0.77	0

品种 Variety	处理Treatment	PC1	PC2	PC3	综合值Comprehensive value（F）
Alamo加倍体Alamo syndiploid	A1B1	-0.58	2.66	-0.24	1.84
	A1B2	0.23	1.62	-1.09	0.76
	A1B3	0.44	1.44	-0.21	1.67
	A1B4	-0.48	0.66	1.15	1.34
	A2B1	-2.00	0.91	1.33	0.25
	A2B2	-2.78	-0.77	1.86	-1.69
	A2B3	-1.92	-0.84	1.28	1.48
	A2B4	-0.93	-0.16	0.02	-1.07
Alamo	A1B1	-0.30	1.66	-1.33	0.03
	A1B2	0.21	1.00	-1.01	0.20
	A1B3	0.07	0.63	-1.21	-0.50
	A1B4	0.38	0.56	0.69	1.62
	A2B1	-2.24	-0.62	0.56	-2.29
	A2B2	-1.29	-0.82	-0.44	-2.55
	A2B3	-0.60	-0.68	-0.86	-2.15
	A2B4	-0.29	-0.70	-1.23	-2.22
Pathfinder	A1B1	1.62	0.62	0.65	2.89
	A1B2	2.89	0.05	1.71	4.64
	A1B3	2.79	-0.45	1.38	3.72
	A1B4	2.78	-0.29	0.98	3.46
	A2B1	0.08	-1.40	-0.87	-2.19
	A2B2	0.57	-2.39	-0.44	-2.26
	A2B3	0.94	-1.48	-1.10	-1.64
	A2B4	0.42	-1.22	-1.58	-2.38

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