草地贪夜蛾在玉米-小麦寄主转换中的转录组分析

doi:10.3969/j.issn.1004-1524.2022.06.14

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (6): 1236-1247.DOI: 10.3969/j.issn.1004-1524.2022.06.14

草地贪夜蛾在玉米-小麦寄主转换中的转录组分析

王斯亮¹(), 邵越², 闫成进¹

1.温州科技职业学院农业与生物技术学院,浙江温州 325000
2.温州市植物保护与土壤肥料管理站,浙江温州 325000

收稿日期:2021-05-06 出版日期:2022-06-25 发布日期:2022-06-30
作者简介:王斯亮（1991—）,女,浙江温州人,博士,讲师,研究方向为昆虫分子生物学。E-mail: wsl512935@163.com
基金资助:
温州市重大科技创新攻关项目(ZN2019002);浙江省教育厅一般科研项目(Y201942802);温州科技职业学院博士启动项目

Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation

WANG Siliang¹(), SHAO Yue², YAN Chengjin¹

1. College of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, Wenzhou 325000, Zhejiang, China
2. Plant Protection and Soil Fertilizer Management Station of Wenzhou, Wenzhou 325000, Zhejiang, China

Received:2021-05-06 Online:2022-06-25 Published:2022-06-30

摘要/Abstract

摘要：

草地贪夜蛾(Spodoptera furgiperda)是重大入侵多食性害虫,在我国南方地区可以周年繁殖。过去的调查和研究表明了小麦有成为草地贪夜蛾越冬寄主的风险。为了探究草地贪夜蛾取食小麦的适应性的内在机制,本研究通过转录组测序技术,以取食玉米的草地贪夜蛾幼虫为对照组,分析比较草地贪夜蛾在转换取食小麦和人工饲料后的表达谱差异。共组装得到66 407条Unigene,其中18 868条获得功能注释。取食小麦和人工饲料的幼虫中,分别找到542条和6 863条差异表达基因。对差异表达基因进行分析,发现大量表皮相关基因表达量在取食小麦的幼虫中显著上调,而在取食人工饲料的幼虫中下调。同时发现细胞色素P450基因在取食小麦和人工饲料的幼虫中均有下调表达。本研究为草地贪夜蛾为害小麦越冬的风险评估工作提供信息和参考。

关键词: 草地贪夜蛾, 转录组, 取食, 小麦, 人工饲料

Abstract:

Spodoptera furgiperda is a major invading pest. In the south of China, S. furgiperda can survive and multiply through whole year. Previous investigations and research revealed the risk that wheat was overwintering host of S. furgiperda. To investigate the internal mechanism of feeding fitness of S. furgiperda to wheat, transcriptomes of S. furgiperda larva feeding on corn, wheat and artificial diet were sequenced and compared. Totally, 66407 unigenes were obtained after assembly and 18868 unigenes were functionally annotated. Respective 542 and 6863 differentially expressed genes were found in wheat-feeding larva and artificial-diet-feeding larva, comparing with corn-feeding larva. Analysis of differentially expressed genes showed that lots of cuticle-related genes up regulated in wheat-feeding larva, while down regulated in artificial-diet-feeding larva. Cytochrome P450 genes were found down regulated in both wheat-feeding and artificial-diet-feeding larva. This study provides information and reference to risk assessment that S. furgiperda damage wheat through overwintering stage.

Key words: Spodoptera furgiperda, transcriptome, feeding, wheat, artificial diet

中图分类号:

S433.4

王斯亮, 邵越, 闫成进. 草地贪夜蛾在玉米-小麦寄主转换中的转录组分析[J]. 浙江农业学报, 2022, 34(6): 1236-1247.

WANG Siliang, SHAO Yue, YAN Chengjin. Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1236-1247.

图/表 10

表1 草地贪夜蛾幼虫转录组测序结果（Clean Data）

Table 1 Sequence data of Spodoptera furgiperda larva transcriptome (Clean Data)

样本 Sample	Read总数 Total reads	碱基总数 Total base	GC(%)	Q20(%)	Q30(%)
FAWsl1	23 478 269	7 043 480 700	49.1	97.52	92.99
FAWsl2	23 868 394	7 160 518 200	50.23	97.6	93.16
FAWsl3	23 171 989	6 951 596 700	46.52	97.14	92.16
FAWw1	24 749 086	7 424 725 800	49.76	97.48	92.89
FAWw2	21 784 418	6 535 325 400	48.55	97.48	92.98
FAWw3	25 620 055	7 686 016 500	49.01	97.7	93.41
FAWym1	26 381 427	7 914 428 100	47.86	97.5	92.99
FAWym2	23 284 104	6 985 231 200	47.75	97.47	92.91
FAWym3	22 446 880	6 734 064 000	47.81	97.29	92.56

表2 草地贪夜蛾幼虫转录组组装结果（Unigene）

Table 2 Assembly results of Spodoptera furgiperda larva transcriptome (Unigene)

指标Index	数据Data
N50 Length（nt)	1 768
Total Number	66 407
Mean Length	1 063.99
300-500	28 559(43.01%)
500-1 000	19 891(29.95%)
1 000-2 000	9 546(14.37%)
2 000+	8 411(12.67%)

表3 草地贪夜蛾幼虫转录组Unigene功能注释

Table 3 Functional annotation of unigenes in Spodoptera furgiperda larva transcriptome

数据库Database	基因总数Number of genes	百分比Percentage	300≤length<1000	length≥1000
COG	4 961	26.29%	736	4 225
GO	10 129	53.68%	2 044	8 085
KEGG	17 615	93.36%	5 902	11 713
KOG	10 271	54.44%	2 068	8 203
Pfam	11 218	59.46%	2 197	9 021
Swissprot	10 910	57.82%	2 215	8 695
NR	18 645	98.82%	6 693	11 952
所有注释Total annotations	18 868		6 867	12 001

图1 草地贪夜蛾幼虫转录组Unigene在NR数据库的同源物种分布

Fig. 1 Species distribution of the unigenes in the transcriptome of Spodoptera frugiperda mapped against the NR database

表4 差异表达基因相关统计

Table 4 Statistics of differential expressed genes

样本 Sample	差异表达基因 Differential expressed genes			有注释的差异表达基因 Annotated differential expressed genes
	总数 Total	上调 Up-regulated	下调 Down-regulated	总数 Total	上调 Up-regulated	下调 Down-regulated
FAWsl	6 863	2 996	3 867	4 607	2 364	2 243
FAWw	542	308	234	432	263	169

表5 特异性差异表达基因与共同差异表达基因

Table 5 Specific differential expressed genes and common differential expressed genes in FAWw and FAWsl

样本 Sample	FAWw特异性差异表达基因 Specific differential expressed genes in FAWw	共同差异表达基因 Common differential expressed genes			FAWsl特异性差异表达基因 Specific differential expressed genes in FAWsl
	FAWw特异性差异表达基因 Specific differential expressed genes in FAWw	FAWsl中反方向* Reverse direction in FAWsl	同方向 Same direction	FAWw中反方向 Reverse direction in FAWw
上调 Up-regulated	87	94	82	30	2 131
下调 Down-regulated	41	30	98	94	2 172

表6 草地贪夜蛾取食小麦后（FAWw）的功能性差异表达基因

Table 6 Functional differential expressed genes in FAWw

基因 gene	上调 Up-regulated				下调 Down-regulated
	总共 Total	特异 Specific	FAWsl中下调down-regulated in FAWsl	共同 Common	总共 Total	特异 Specific	FAWsl中上调 up-regulated in FAWsl	共同 Common
表皮相关基因 Cuticle-related genes	32	5	27	0	3	0	1	2
细胞色素P450基因 Cytochrome P450 genes	3	2	1	0	13	4	1	8

图2 差异表达基因GO富集分析 A,草地贪夜蛾幼虫取食小麦后（FAWw）的差异表达基因GO富集分析;B,草地贪夜蛾幼虫取食人工饲料后（FAWsl）的差异表达基因GO富集分析。

Fig.2 GO enrichment of differential expressed genes A, GO enrichment of differential expressed genes in FAWw; B, GO enrichment of differential expressed genes in FAWsl.

图3 草地贪夜蛾取食人工饲料后（FAWsl）的差异表达基因KEGG代谢通路富集分析 A,FAWsl上调表达基因的KEGG代谢通路富集分析;B,FAWsl下调表达基因的KEGG代谢通路富集分析。

Fig.3 KEGG pathway enrichment in FAWsl A, KEGG pathway enrichment in of the up-regulated genes in FAWsl; B, KEGG pathway enrichment in of the down-regulated genes in FAWsl.

图4 草地贪夜蛾取食小麦后（FAWw）的差异表达基因KEGG代谢通路富集分析 A,FAWw上调表达基因的KEGG代谢通路富集分析;B,FAWw下调表达基因的KEGG代谢通路富集分析。

Fig.4 KEGG pathway enrichment in FAWw A, KEGG pathway enrichment in of the up-regulated genes in FAWw; B, KEGG pathway enrichment in of the down-regulated genes in FAWw.

参考文献 31

[1]	GOERGEN G, KUMAR P L, SANKUNG S B, et al. First report of outbreaks of the fall armyworm Spodoptera frugiperda (J E Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in west and central Africa[J]. PLoS One, 2016, 11(10): e0165632. DOI URL
[2]	MONTEZANO D G, SPECHT A, SOSA-GÓMEZ D R, et al. Host plants ofSpodoptera frugiperda(Lepidoptera: Noctuidae) in the americas[J]. African Entomology, 2018, 26(2): 286-300. DOI URL
[3]	DUMAS P, LEGEAI F, LEMAITRE C, et al. Spodoptera frugiperda (Lepidoptera: Noctuidae) host-plant variants: two host strains or two distinct species?[J]. Genetica, 2015, 143(3): 305-316. DOI URL
[4]	张磊, 柳贝, 姜玉英, 等. 中国不同地区草地贪夜蛾种群生物型分子特征分析[J]. 植物保护, 2019, 45(4): 20-27.
	ZHANG L, LIU B, JIANG Y Y, et al. Molecular characterization analysis of fall armyworm populations in China[J]. Plant Protection, 2019, 45(4): 20-27. (in Chinese with English abstract)
[5]	赵雪晴, 陈福寿, 尹艳琼, 等. 草地贪夜蛾在云南元谋县青稞、燕麦、糜子田的发生为害特征[J]. 植物保护, 2020, 46(2): 216-221.
	ZHAO X Q, CHEN F S, YIN Y Q, et al. Occurrence and damage characteristics of Spodoptera frugiperda on highland barley, oat and prosomillet in Yuanmou County, Yunnan Province[J]. Plant Protection, 2020, 46(2): 216-221. (in Chinese with English abstract)
[6]	孙东磊, 文明富, 李继虎, 等. 广东蔗区草地贪夜蛾为害调查初报[J]. 环境昆虫学报, 2019, 41(6): 1155-1162.
	SUN D L, WEN M F, LI J H, et al. Preliminary report on the investigation of the damage of Spodoptera frugiperda in the sugarcane area of Guangdong Province[J]. Journal of Environmental Entomology, 2019, 41(6): 1155-1162. (in Chinese with English abstract)
[7]	何莉梅, 赵胜园, 吴孔明. 草地贪夜蛾取食为害花生的研究[J]. 植物保护, 2020, 46(1): 28-33.
	HE L M, ZHAO S Y, WU K M. Study on the damage of fall armyworm, Spodoptera frugiperda to peanut[J]. Plant Protection, 2020, 46(1): 28-33. (in Chinese with English abstract)
[8]	赵猛, 杨建国, 王振营, 等. 山东发现草地贪夜蛾为害马铃薯[J]. 植物保护, 2019, 45(6): 84-86.
	ZHAO M, YANG J G, WANG Z Y, et al. Spodoptera frugiperda were found damaging potato in Shandong Province[J]. Plant Protection, 2019, 45(6): 84-86. (in Chinese with English abstract)
[9]	刘银泉, 王雪倩, 钟宇巍. 草地贪夜蛾在浙江为害甘蓝[J]. 植物保护, 2019, 45(6): 90-91.
	LIU Y Q, WANG X Q, ZHONG Y W. Fall armyworm Spodoptera frugiperda feeding on cabbage in Zhejiang[J]. Plant Protection, 2019, 45(6): 90-91. (in Chinese with English abstract)
[10]	王磊, 陈科伟, 钟国华, 等. 重大入侵害虫草地贪夜蛾发生危害、防控研究进展及防控策略探讨[J]. 环境昆虫学报, 2019, 41(3): 479-487.
	WANG L, CHEN K W, ZHONG G H, et al. Progress for occurrence and management and the strategy of the fall armyworm Spodoptera frugiperda (Smith)[J]. Journal of Environmental Entomology, 2019, 41(3): 479-487. (in Chinese with English abstract)
[11]	GARCIA A G, GODOY W A C, THOMAS J M G, et al. Delimiting strategic zones for the development of fall armyworm (Lepidoptera: Noctuidae) on corn in the state of Florida[J]. Journal of Economic Entomology, 2017, 111(1): 120-126. DOI URL
[12]	林伟, 徐淼锋, 权永兵, 等. 基于MaxEnt模型的草地贪夜蛾适生性分析[J]. 植物检疫, 2019, 33(4): 69-73.
	LIN W, XU M F, QUAN Y B, et al. Potential geographic distribution of Spodoptera frugiperda in China based on MaxEnt model[J]. Plant Quarantine, 2019, 33(4): 69-73. (in Chinese with English abstract)
[13]	秦誉嘉, 蓝帅, 赵紫华, 等. 迁飞性害虫草地贪夜蛾在我国的潜在地理分布[J]. 植物保护, 2019, 45(4): 43-47.
	QIN Y J, LAN S, ZHAO Z H, et al. Potential geographical distribution of the fall armyworm (Spodoptera frugiperda) in China[J]. Plant Protection, 2019, 45(4): 43-47. (in Chinese with English abstract)
[14]	黄芊, 凌炎, 蒋婷, 等. 草地贪夜蛾对三种寄主植物的取食选择性及其适应性研究[J]. 环境昆虫学报, 2019, 41(6): 1141-1146.
	HUANG Q, LING Y, JIANG T, et al. Feeding preference and adaptability of Spodoptera frugiperda on three host plant[J]. Journal of Environmental Entomology, 2019, 41(6): 1141-1146. (in Chinese with English abstract)
[15]	吴正伟, 师沛琼, 曾永辉, 等. 3种寄主植物饲养的草地贪夜蛾种群生命表[J]. 植物保护, 2019, 45(6): 59-64.
	WU Z W, SHI P Q, ZENG Y H, et al. Population life tables of Spodoptera frugiperda (Lepidoptera: Noctuidae) fed on three host plants[J]. Plant Protection, 2019, 45(6): 59-64. (in Chinese with English abstract)
[16]	姚领, 房敏, 李晓萌, 等. 草地贪夜蛾对三种杂草的产卵和取食选择性[J]. 植物保护, 2020, 46(4): 181-184.
	YAO L, FANG M, LI X M, et al. Oviposition and feeding selectivity of Spodoptera frugiperda to three weeds[J]. Plant Protection, 2020, 46(4): 181-184. (in Chinese with English abstract)
[17]	齐国君, 黄德超, 王磊, 等. 广东省草地贪夜蛾冬季发生特征及周年繁殖区域研究[J]. 环境昆虫学报, 2020, 42(3): 573-582.
	QI G J, HUANG D C, WANG L, et al. The occurrence characteristic in winter and year-round breeding region of the fall armyworm, Spodoptera frugiperda (J.E.Smith) in Guangdong Province[J]. Journal of Environmental Entomology, 2020, 42(3): 573-582. (in Chinese with English abstract)
[18]	徐丽娜, 胡本进, 苏卫华, 等. 安徽发现草地贪夜蛾为害早播小麦[J]. 植物保护, 2019, 45(6): 87-89.
	XU L N, HU B J, SU W H, et al. Fall armyworm damaging early sowing wheat in Anhui Province[J]. Plant Protection, 2019, 45(6): 87-89. (in Chinese with English abstract)
[19]	李艳朋, 李猛, 刘鸿恒, 等. 草地贪夜蛾在江苏北部早播麦田的发生与防治[J]. 植物保护, 2020, 46(2): 212-215.
	LI Y P, LI M, LIU H H, et al. Occurrence and control of Spodoptera frugiperda in early sowing wheat field in northern Jiangsu Province[J]. Plant Protection, 2020, 46(2): 212-215. (in Chinese with English abstract)
[20]	张智, 林培炯, 陈智勇, 等. 小麦中后期草地贪夜蛾为害特征观察[J]. 植物保护, 2021, 47(5): 297-301.
	ZHANG Z, LIN P J, CHEN Z Y, et al. Observation on the damage characteristics of Spodoptera frugiperda to wheat in middle and late stages[J]. Plant Protection, 2021, 47(5): 297-301. (in Chinese with English abstract)
[21]	巴吐西, 张云慧, 张智, 等. 草地贪夜蛾对小麦和玉米的产卵选择性及其种群生命表[J]. 植物保护, 2020, 46(1): 17-23.
	BA T X, ZHANG Y H, ZHANG Z, et al. The host preference and population life tables of Spodoptera frugiperda (Lepidoptera: Noctuidae) fed on maize and wheat[J]. Plant Protection, 2020, 46(1): 17-23. (in Chinese with English abstract)
[22]	吕亮, 李雨晴, 陈从良, 等. 草地贪夜蛾幼虫在玉米和小麦上的取食和生长发育特性比较[J]. 昆虫学报, 2020, 63(5): 597-603.
	LÜ L, LI Y Q, CHEN C L, et al. Comparison of the feeding and growth characteristics of larvae of the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), on corn and wheat[J]. Acta Entomologica Sinica, 2020, 63(5): 597-603. (in Chinese with English abstract)
23	李子园, 戴钎萱, 邝昭琅, 等. 3种人工饲料对草地贪夜蛾生长发育及繁殖力的影响[J]. 环境昆虫学报, 2019, 41(6): 1147-1154.
	LI Z Y, DAI Q X, KUANG Z L, et al. Effects of three artificial diets on development and reproduction of the fall armyworm Spodoptera frugiperda (J.E. Smith)[J]. Journal of Environmental Entomology, 2019, 41( 6): 1147-1154. (in Chinese with English abstract)
[24]	RAMSEY J S, RIDER D S, WALSH T K, et al. Comparative analysis of detoxification enzymes in Acyrthosiphon pisum and Myzus persicae[J]. Insect Molecular Biology, 2010, 19( Suppl.2): 155-164. (in Chinese with English abstract)
[25]	GOUIN A, BRETAUDEAU A, NAM K,et al.Two genomes of highly polyphagous lepidopteran pests (Spodoptera frugiperda, Noctuidae) with different host-plant ranges[J]. Scientific Reports, 2017, 7: 11816. DOI URL
[26]	梅洋, 杨义, 叶昕海, 等. 草地贪夜蛾解毒代谢相关基因家族的进化分析[J]. 环境昆虫学报, 2019, 41(4): 727-735.
	MEI Y, YANG Y, YE X H, et al. Evolutionary analysis of detoxification gene families of Spodoptera frugiperda[J]. Journal of Environmental Entomology, 2019, 41(4): 727-735. (in Chinese with English abstract)
[27]	王芹芹, 崔丽, 王立, 等. 草地贪夜蛾对小麦的为害风险: 取食为害性及解毒酶活性变化初探[J]. 植物保护, 2020, 46(1): 63-68.
	WANG Q Q, CUI L, WANG L, et al. Risk analysis of the harm of the fall armyworm, Spodoptera frugiperda to wheat: the harmfulness of feeding and change of detoxification enzyme activities[J]. Plant Protection, 2020, 46(1): 63-68. (in Chinese with English abstract)
28	邱良妙, 刘其全, 杨秀娟, 等. 草地贪夜蛾对水稻和玉米的取食和产卵选择性与适合度[J]. 昆虫学报, 2020, 63(5): 604-612.
	QIU L M, LIU Q Q, YANG X J, et al. Feeding and oviposition preference and fitness of the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), on rice and maize[J]. Acta Entomologica Sinica, 2020, 63(5):604-612. (in Chinese with English abstract)
29	PAN P L, YE Y X, LOU Y H,et al. A comprehensive omics analysis and functional survey of cuticular proteins in the brown planthopper[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(20): 5175-5180.
30	陈斌,乔梁. 昆虫表皮蛋白基因研究进展[J]. 昆虫学报, 2014, 57(9): 1084-1093.
	LIANG X, CHEN B, QIAO L. Research progress in insect cuticular protein genes[J]. Acta Entomologica Sinica, 2014, 57( 9): 1084-1093. (in Chinese with English abstract)
31	VANNINI L, DUNN W A, REED T W, et al.Changes in transcript abundance for cuticular proteins and other genes three hours after a blood meal in Anopheles gambiae[J]. Insect Biochemistry and Molecular Biology, 2014, 44: 33-43. DOI URL

草地贪夜蛾在玉米-小麦寄主转换中的转录组分析

Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation

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