香榧扦插生根解剖学与生理相关酶活性

doi:10.3969/j.issn.1004-1524.2022.09.14

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (9): 1955-1966.DOI: 10.3969/j.issn.1004-1524.2022.09.14

香榧扦插生根解剖学与生理相关酶活性

金侯定¹^,²(), 郑春颖³, 华斌⁴, 俞晨良¹, 李柯豫¹, 喻卫武¹^,^*()

1.浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州 311300
2.上海建设管理职业技术学院园林技术与艺术学院, 上海 200232
3.安吉县灵峰寺林场,浙江安吉 313300
4.杭州市富阳区农林资源保护中心,浙江杭州 311400

收稿日期:2021-10-08 出版日期:2022-09-25 发布日期:2022-09-30
通讯作者: 喻卫武
作者简介:*喻卫武,E-mail: yww888@zafu.edu.cn
金侯定(1990—),女,江苏无锡人,硕士,工程师,研究方向为园林植物。E-mail: 573513667@qq.com
基金资助:
香榧新品种选育专项(2021C02066-11)

Rooting anatomy and physiological enzyme activity of Torreya grandis cuttings

JIN Houding¹^,²(), ZHENG Chunying³, HUA Bin⁴, YU Chenliang¹, LI Keyu¹, YU Weiwu¹^,^*()

1. The State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China
2. College of Landscape Technology and Art, Shanghai Construction Management Vocational and Technical College, Shanghai 200232, China
3. Lingfengsi Forest Farm in Anji County, Anji 313300, Zhejiang, China
4. Agricultural and Forestry Resources Protection Center in Fuyang District of Hangzhou, Hangzhou 311400, China

Received:2021-10-08 Online:2022-09-25 Published:2022-09-30
Contact: YU Weiwu

摘要/Abstract

摘要：

近年来香榧种植规模大幅度增加,但香榧多嫁接繁殖,生产周期长,而扦插繁殖在生产上尚未广泛应用。为解决香榧生产上苗木供应短缺的问题,深化香榧繁殖方面的研究,从解剖学与生根过程中相关酶活性变化开展研究,并且比较了标典3721扦插专用生根液和双吉尔6号生根粉(GGR6)对香榧扦插生根率的影响。结果表明,香榧一年生枝条内有厚壁组织但不连续,无潜伏根原基,根原基起源于愈伤组织;在扦插后约一个月能较快形成根原基,但是其发育并形成不定根突破愈伤组织所需时间较长。标典处理的香榧插穗生根率和愈伤率高于GGR6处理和对照。香榧插穗基部皮层的过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)活性变化与插穗生根密切相关。扦插初期,POD、SOD活性上升,IAAO活性则下降;PPO活性先下降后上升。随着切口愈伤组织的形成和根原基的发生,POD、SOD活性逐渐下降,IAAO活性、PPO活性上升。扦插后期,POD和IAAO活性的上升促使不定根的伸长和生长,SOD活性又开始恢复,PPO活性趋于平缓。香榧扦插生根的诱导与生长过程中,这些酶活性的变化起着重要的作用。扦插初期各处理可溶性蛋白含量均呈上升趋势,随着根原基的发生与不定根的形成与伸长又呈下降趋势。研究结果可以为香榧扦插技术的研发提供理论依据。

关键词: 香榧, 扦插, 解剖学, 生根, 酶活性

Abstract:

In recent years, planting scale of Torreya grandis has increased significantly. T. grandis is mostly grafted and propagated, and the production cycle is long, while cutting propagation has not been widely used in production.To solve the shortage of seedlings in T. grandis production, the research on its propagation was deepened in this paper, anatomy and changes of related enzyme activities during rooting process were carried out. Effects of Biaodian 3721 rooting solution and Shuangjil No. 6 rooting powder (GGR6) on the rooting rate of T. grandis were compared. The results showed that there were thick-walled tissues but discontinuous in the one-year-old branches of T. grandis, and no latent root primordium. The root primordium originated from callus. The root primordium could be formed quickly about one month after cuttings, but it took a very long time for its development and formation of adventitious roots to break through the callus. The rooting rate and callus rate of T. grandis cuttings in Biaodian treatment were higher than those in GGR6 treatment and control. Changes of peroxidase (POD), polyphenol oxidase (PPO) and indole acetate oxidase (IAAO) activities in the cortex at the base of the cuttings were closely related to the rooting of the cuttings. In the early stage of cutting, the activity of POD and SOD increased, while the activity of IAAO decreased; the activity of PPO first decreased and then increased. With the formation of incision callus and root primordium, the activity of POD and SOD gradually decreased, and the activity of IAAO and PPO increased. In the late cutting period, the increase of POD and IAAO activities promoted the elongation and growth of adventitious roots, SOD activity began to recover, and PPO activity tended to be flat. The soluble protein content of each treatment showed an upward trend at the initial stage of cutting, and showed a downward trend with the occurrence of root primordium and the formation and elongation of adventitious roots. The changes of these enzyme activities played an important role in the induction of cutting rooting and growth of T. grandis. The result provided a theoretical basis for the research and development of the cutting technology of torreya cuttings.

Key words: Torreya grandis, cutting, anatomy, rootting, enzyme activity

中图分类号:

S664.5

金侯定, 郑春颖, 华斌, 俞晨良, 李柯豫, 喻卫武. 香榧扦插生根解剖学与生理相关酶活性[J]. 浙江农业学报, 2022, 34(9): 1955-1966.

JIN Houding, ZHENG Chunying, HUA Bin, YU Chenliang, LI Keyu, YU Weiwu. Rooting anatomy and physiological enzyme activity of Torreya grandis cuttings[J]. Acta Agriculturae Zhejiangensis, 2022, 34(9): 1955-1966.

图/表 6

图1 香榧插穗的解剖学观察 a,茎横切;b,韧皮部与形成层;c,茎表皮;d,叶迹或枝迹。Cl,表皮层;Co,皮层;Ph,韧皮部;Php,韧皮薄壁组织;Rd,树脂道;CrF,含晶韧皮纤维;C,形成层;Xy,木质部;Pi,髓;LT/BT,叶迹或枝迹;Pi,髓;Vc,维管柱。

Fig.1 Anatomical observation of Torreya grandis ‘Merrillii’ cuttings a, Transectional shoot; b, Phloem and callus; c, Cuticle of the shoot; d, Leaf trace or branch shoot. Cl, Cuticular layer; Co, Cortex; Ph, Phloem; Php, Parenchyma in phloem; Rd, Resin duct; CrF, Crystalliferous phloem fiber; C, Cambial layer; Xy, Xylem; Pi, Pith; LT/BT, Leaf trace or branch trace; Vc, Vascular column.

图2 香榧插穗不定根形成的外部形态变化过程 a,无愈伤组织(7 d);b,出现薄薄一层愈伤组织(15 d);c,愈伤组织明显隆起,颜色为黄褐色(20 d);d,基部愈伤组织继续膨大(35 d);e,基部底端出现黄色突起(45 d);f,白色不定根突破愈伤组织表面(55 d);g,不定根伸长(65 d);h,出现一级侧根(95 d)。图中标尺均表示0.5 cm。

Fig.2 Morphological changes in adventitious roots of Torreya grandis ‘Merrillii’ cuttings a, No callus observed (7 days after cutting); b, A thin layer of callus observed (15 days after cutting); c, Obviously protruded yellow-brown callus (20 days after cutting); d, Continuously swelling callus (35 days after cutting); e, The yellow swelling observed at the bottom of the cut (45 days after cutting); f, White adventitious roots breaking through the callus surface (55 days after cutting); g, Elongating adventitious roots observed on half of the cuttings (65 days after cutting); h, The first grade lateral roots observed (95 days after cutting). Bar scale=0.5 cm.

图3 香榧扦插不定根发育的解剖学观察 a、b,愈伤组织形成部位;c,根原基;d,不定根与茎维管束连接;e,不定根横切;f,不定根纵切。Ca,愈伤组织;RPIC,根原基原始细胞;Cl,表皮层;Co,皮层;C,形成层;Xy,木质部;Pi,髓;Vc,维管柱;Ar,不定根;Per,中柱鞘;PX,初生木质部;PPh,初生韧皮部;RH,根毛;AM,顶端分生组织;M,分生区;Ez,伸长区;Ph,韧皮部。

Fig.3 Anatomical observation of developing adventitious roots of Torreya grandis‘Merrillii’ cuttings a and b, Formation of callus; c, Root primordia; d, Connection between adventitious roots and vascular bundle; e and f, Transverse and longitudinal sections of adventitious roots. Ca, Callus; RPIC, Initial cells in root primordia; Cl, Cuticular layer; Co, Cortex; C, Cambial layer; Xy, Xylem; Pi, Pith; Vc, Vascular column; Ar, Adventitious root; Per, Pericycle; PX, Primary xylem; PPh, Primary phloem; RH, Root hair; AM, Apical meristem; M, Meristem; Ez, Elongation zone; Ph, Phloem.

表1 不同处理对香榧扦插生根的影响

Table 1 Effects of different treatments on cutting rooting of Torreya grandis

处理 Treatments	处理总数 Total	生根数 Rooting number	愈伤数 Callus number	死亡数 Deaths number
对照Control	90	17	34	39
标典Biaodian	90	19	54	17
GGR6	90	14	16	61

图4 香榧插穗生根过程中相关酶活性的变化数据以鲜重计。下同。

Fig.4 Changes of related enzyme activities during rooting of Torreya grandis cuttings Data was detected based on fresh weight. The same as below.

图5 香榧插穗生根过程中可溶性蛋白含量变化

Fig.5 Changes of soluble protein contents during rooting of Torreya grandis cuttings

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香榧扦插生根解剖学与生理相关酶活性

Rooting anatomy and physiological enzyme activity of Torreya grandis cuttings

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