柳叶蜡梅多糖对大鼠急性胃黏膜损伤的保护机制研究

doi:10.3969/j.issn.1004-1524.20240827

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (5): 1009-1016.DOI: 10.3969/j.issn.1004-1524.20240827

柳叶蜡梅多糖对大鼠急性胃黏膜损伤的保护机制研究

陈琳¹(), 顾青²^,^*()

1.浙江商业职业技术学院食品研发中心,浙江杭州 310053
2.浙江工商大学食品与生物工程学院,浙江省食品微生物技术研究重点实验室,浙江杭州 310018

收稿日期:2024-09-22 出版日期:2025-05-25 发布日期:2025-06-11
作者简介:陈琳(1985—),女,黑龙江牡丹江人,博士,副教授,研究方向为食品生物技术。E-mail: chenlinlucky@163.com
通讯作者: *顾青,E-mail:guqing2002@hotmail.com
基金资助:
浙江省哲学社会科学规划课题(24NDJC184YB);浙江省自然科学基金(LTGN24C200009)

Gastroprotective effect of polysaccharides extracted from Chimonanthus salicifolius on ethanol-induced gastric injury in rats

CHEN Lin¹(), GU Qing²^,^*()

1. Department of Food Research, Zhejiang Business College, Hangzhou 310053, China
2. Key Laboratory for Food Microbial Technology of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China

Received:2024-09-22 Online:2025-05-25 Published:2025-06-11

摘要/Abstract

摘要：

通过构建乙醇致急性胃溃疡大鼠模型,解析新型食品原料——柳叶蜡梅多糖保护胃损伤的作用机制。研究表明,柳叶蜡梅多糖的主要成分为甘露糖、鼠李糖、葡萄糖、半乳糖和海藻糖。大鼠灌胃柳叶蜡梅多糖低剂量组(200 mg·kg^-1,以体重计)和高剂量组(400 mg·kg^-1,以体重计)28 d可缓解乙醇致急性胃溃疡,显著(P<0.05)降低胃溃疡指数。与模型组相比,柳叶蜡梅多糖高剂量组显著提高超氧化物歧化酶(SOD)活性和前列腺素E2(PGE2)、表皮生长因子(EGF)的含量,降低丙二醛(MDA)含量,抑制肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)、白介素1β(IL-1β)的产生。柳叶蜡梅多糖提取物通过调控丝裂原活化蛋白激酶(MAPKs)和基质金属蛋白酶(MMPs)信号通路,缓解大鼠胃部氧化应激反应和乙醇引致的胃黏膜损伤。

关键词: 柳叶蜡梅, 多糖, 胃溃疡, 乙醇

Abstract:

In this study, we established a rat model of ethanol-induced gastric ulcer, to examine the gastroprotective effect of polysaccharides extracted from Chimonanthus salicifolius(CSP), and reveal the related mechanism. It was shown that the main components of CSP were mannose, rhamnose, glucose, galactose and trehalose. Oral gavage with low (200 mg·kg^-1, based on body weight) or high (400 mg·kg^-1, based on body weight ) dose CSP for 28 days prevented the formation of gastric ulcer, as the ulcer index was significantly (P<0.05) decreased. Compared with the model group, high dose CSP treatment significantly enhanced the activity of superoxide dismutase (SOD) and the contents of prostaglandin E2 (PGE2) and epidermal growth factor (EGF), decreased the malonaldehyde (MDA) content, and inhibited the formation of tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β). CSP could relieve the gastric oxidative stress in rats by regulating the signaling pathways of mitogen-activated protein kinases (MAPKs) and matrix metalloproteinases (MMPs), hence protect rats from the ethanol-induced gastric damage.

Key words: Chimonanthus salicifolius, polysaccharides, gastric ulcer, ethanol

中图分类号:

陈琳, 顾青. 柳叶蜡梅多糖对大鼠急性胃黏膜损伤的保护机制研究[J]. 浙江农业学报, 2025, 37(5): 1009-1016.

CHEN Lin, GU Qing. Gastroprotective effect of polysaccharides extracted from Chimonanthus salicifolius on ethanol-induced gastric injury in rats[J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 1009-1016.

图/表 6

图1 柳叶蜡梅多糖的组成分析 1~7依次代表甘露糖、鼠李糖、葡萄糖、半乳糖、木糖、阿拉伯糖、海藻糖。A图展示单糖标准物质,B图展示柳叶蜡梅多糖。

Fig.1 Composition analysis of Chimonanthus salicifolius polysaccharides 1-7 represent mannose, rhamnose, glucose, galactose, xylose, arabinose and trehalose, respectively. Fig. A shows the result of reference standard of monosaccharides, while Fig. B shows the result of Chimonanthus salicifolius polysaccharides.

图2 不同处理的胃组织形态学影像 A,对照组;B,模型组;C,奥美拉唑组;D,柳叶蜡梅多糖低剂量组;E,柳叶蜡梅多糖高剂量组。

Fig.2 Histomorphology images of stomach tissue under treatments A, Control group; B, Model group; C, Omeprazole group; D, Low dose Chimonanthus salicifolius polysaccharides group; E, High dose Chimonanthus salicifolius polysaccharides group.

图3 不同处理胃组织的溃疡指数(UI) C,对照组;M,模型组;O,奥美拉唑组;L,柳叶蜡梅多糖低剂量组;H,柳叶蜡梅多糖高剂量组。柱上无相同字母的表示处理间差异显著(P<0.05)。

Fig.3 Ulcer index (UI) of gastric tissues under treatments C, Control group; M, Model group; O, Omeprazole group; L, Low dose Chimonanthus salicifolius polysaccharides group; H, High dose Chimonanthus salicifolius polysaccharides group. Bars marked without the same letters indicate significant difference at P<0.05.

图4 不同处理下胃黏膜的组织学观察结果从左至右各列依次展示对照组(C)、模型组(M)、奥美拉唑组(O)、柳叶蜡梅多糖低剂量组(L)、柳叶蜡梅多糖高剂量组(H)的结果。上面一行与下面一行的放大倍数分别为100倍和400倍。

Fig.4 Histological observation of gastric mucosal under treatments Lines from left to right show the results of control group (C), model group (M), omeprazole group (O), low dose Chimonanthus salicifolius polysaccharides group (L), high dose Chimonanthus salicifolius polysaccharides group (H), respectively. The magnification multiples in the upper row and lower row are 100 and 400, respectively.

表1 不同处理对大鼠生理生化指标的影响

Table 1 Effects of treatments on biological and biochemical indexes of rats

处理 Treatment	SOD活性 SOD activity/ (U·mg^-1)	MDA含量 MDA content/ (nmol·mg^-1)	EGF含量 EGF content/ (ng·L^-1)	PGE2含量 PEG2 content/ (ng·L^-1)	TNF-α含量 TNF-α content/ (pg·mL^-1)	IL-6含量 IL-6 content/ (pg·mL^-1)	IL-1β含量 IL-1β content/ (pg·mL^-1)
C	258.23±6.36 a	4.68±0.14 c	480.34±56.23 a	418.43±24.65 a	80.24±3.36 b	60.68±3.14 b	20.34±1.23 c
M	176.35±5.24 c	6.56±0.46 a	220.43±53.87 c	303.45±34.23 b	120.35±4.17 a	136.56±6.87 a	50.43±1.87 a
O	192.45±4.87 c	5.25±0.54 bc	368.98±76.23 b	432.25±33.43 a	92.45±3.23 b	75.25±0.54 b	35.12±2.23 b
L	187.65±9.56 c	6.23±0.34 ab	267.98±66.43 bc	450.24±23.45 a	87.25±4.14 b	76.23±0.34 b	37.98±2.43 ab
H	202.36±10.62 b	4.87±0.34 c	428.65±59.76 a	489.34±34.54 a	80.36±8.26 b	66.87±0.34 b	30.65±3.76 b

图5 蛋白质表达的结果 A,基质金属蛋白酶-2(MMP-2);B,磷酸化细胞外调节蛋白激酶(p-ERK);C,基质金属蛋白酶-9(MMP-9);D,磷酸化末端激酶(p-JNK);E,磷酸化丝裂原活化蛋白激酶(p-p38)。GAPDH,甘油醛-3-磷酸脱氢酶。

Fig.5 Result of proteins' expression A, Matrix metalloproteinase-2 (MMP-2); B, Phospho-extracellular regulated protein kinases (p-ERK); C, Matrix metalloproteinase-9 (MMP-9); D, Phospho-c-Jun N-terminal kinase (p-JNK); E, Phospho-mitogen-activated protein kinase (p-p38). GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.

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柳叶蜡梅多糖对大鼠急性胃黏膜损伤的保护机制研究

Gastroprotective effect of polysaccharides extracted from Chimonanthus salicifolius on ethanol-induced gastric injury in rats

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