低温弱光胁迫下AMF和PGPR对紫罗兰生长及营养吸收的影响

doi:10.3969/j.issn.1004-1524.20240734

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (8): 1694-1705.DOI: 10.3969/j.issn.1004-1524.20240734

低温弱光胁迫下AMF和PGPR对紫罗兰生长及营养吸收的影响

师阳阳¹(), 吕丽霞²^,^*(), 脱登峰³

1.山西工程科技职业大学,山西晋中 030619
2.张家口市农业科学院,河北张家口 075000
3.中国林业科学研究院生态保护与修复研究所,北京 100091

收稿日期:2024-08-13 出版日期:2025-08-25 发布日期:2025-09-03
作者简介:师阳阳(1987—),女,山西长治人,硕士,讲师,主要从事林草生态及园林植物研究。E-mail: Kechengyj240323@126.com
通讯作者: *吕丽霞,E-mail:llx08.06@163.com
基金资助:
2023年山西省基础研究计划(自由探索类)项目(202303021222286)

Effects of AMF and PGPR on growth and nutrient absorption of Matthiola incana under low temperature and weak light stress

SHI Yangyang¹(), LYU Lixia²^,^*(), TUO Dengfeng³

1. Shanxi Vocational University of Engineering Science and Technology, Jinzhong 030619, Shanxi, China
2. Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, Hebei, China
3. Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing 100091, China

Received:2024-08-13 Online:2025-08-25 Published:2025-09-03
Contact: LYU Lixia

摘要/Abstract

摘要：

以紫罗兰(Matthiola incana)为试材,比较了常温常光(温度20 ℃,光量子通量密度1 000 μmol·m^-2·s^-1)和低温弱光(温度5 ℃,光量子通量密度100 μmol·m^-2·s^-1)下接种丛枝菌根真菌(AMF)、植物根围促生细菌(PGPR)以及复合接种(AMF+PGPR)处理对紫罗兰生长、营养吸收以及激素代谢的影响,以期为低温弱光胁迫下应用AMF以及PGPR协同技术促进植物生长提供理论依据。结果表明,低温弱光胁迫抑制紫罗兰株高、叶面积以及植株鲜重的增加,光合色素含量下降,氮磷钾含量降低,而接种AMF或/和PGPR处理对紫罗兰生长均具有一定的促进作用。低温弱光胁迫下,AMF+PGPR处理的菌根侵染率较AMF处理增加了7.3百分点,但AMF+PGPR和AMF处理的丛枝着生率、侵入点位数和泡囊数无显著差异;与PGPR处理相比,AMF+PGPR处理的根围土壤中PGPR数量增加52.0%,根内PGPR数量增加50.0%。低温弱光下,与不接种处理相比,AMF+PGPR处理植株的叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量分别增加62.7%、45.0%、59.8%和52.4%;地上部N含量、根系N含量、地上部P含量、根系P含量、地上部K含量、根系K含量分别增加63.3%、206.0%、89.4%、138.6%、108.0%和191.3%。与常温常光处理相比,低温弱光促进了脱落酸(ABA)含量的增加,但导致吲哚乙酸(IAA)含量下降,接种AMF或/和PGPR处理能增加IAA含量并降低ABA含量,其中AMF+PGPR处理下IAA含量增加8.9%,ABA含量下降6.5%;低温弱光下超氧化物歧化酶(SOD)、过氧化物酶(POD)活性下降,而丙二醛(MDA)和过氧化氢(H₂O₂)含量上升,接种AMF或/和PGPR处理有助于增加SOD、POD活性并降低MDA和H₂O₂含量,AMF+PGPR处理下SOD、POD活性增加42.6%和26.8%,MDA以及H₂O₂含量下降24.3%和30.9%。综上,AMF和PGPR具有协同作用,AMF促进PGPR根内和土壤内定殖数量,PGPR增加AMF菌根侵染率,AMF+PGPR处理提高紫罗兰对低温弱光抵抗性的效果优于单一AMF或PGPR处理。

关键词: 丛枝菌根真菌, 植物根围促生细菌, 紫罗兰, 低温, 弱光, 营养物质

Abstract:

Using Matthiola incana as the test material, this study investigated the effects of inoculation with arbuscular mycorrhizal fungi (AMF), plant root growth promoting bacteria (PGPR), and compound inoculation (AMF+PGPR) under normal temperature and light (temperature 20 ℃, photon flux density 1 000 μmol·m^-2·s^-1) and low temperature and weak light (temperature 5 ℃, photon flux density 100 μmol·m^-2·s^-1) on the growth, nutrient absorption, and hormone metabolism of M. incana, with no inoculation (CK) as the control, in order to provide theoretical basis for the application of AMF and PGPR synergistic technology to promote plant growth under low temperature and weak light stress. The results showed that low temperature and weak light stress inhibited the increase in height, leaf area, and fresh weight of M. incana plants, decreased the content of photosynthetic pigments, and reduced the content of nitrogen, phosphorus, and potassium. However, inoculation with AMF and/or PGPR had a certain promoting effect on M. incana growth. Under low temperature and weak light stress, the mycorrhizal infection rate of AMF+PGPR treatment increased by 7.3 percentage points compared with AMF treatment, but there was no significant difference in arbuscular attachment rate, number of invasion points, and number of vesicles between AMF+PGPR and AMF treatments; Compared with PGPR treatment, the number of PGPR in the rhizosphere soil treated with AMF+PGPR increased by 52.0%, and the number of PGPR in the roots increased by 50.0%. Under low temperature and weak light conditions, compared with CK treatment, the content of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids increased by 62.7%, 45.0%, 59.8%, and 52.4%, respectively, under AMF+PGPR treatment; The aboveground N content, root N content, aboveground P content, root P content, aboveground K content, and root K content increased by 63.3%, 206.0%, 89.4%, 138.6%, 108.0%, and 191.3%, respectively. Under low temperature and weak light conditions, the content of IAA in hormone metabolism increased, while the content of ABA decreases. Under AMF+PGPR treatment, the activities of SOD and POD increased by 42.6% and 26.8%, respectively, while the content of MDA and H₂O₂ decreased by 24.3% and 30.9%, respectively. In summary, AMF and PGPR have a synergistic effect. AMF promotes the colonization of PGPR in roots and soil, PGPR increases the arbuscular mycorrhizal infection rate of AMF, and the effect of AMF+PGPR treatment on improving M. incana’s resistance to low temperature and weak light is better than that of single AMF or PGPR treatment.

Key words: arbuscular mycorrhizal fungus, plant growth-promoting rhizobacteria, Matthiola incana, low temperature, weak light, nutrient

中图分类号:

S681.2

师阳阳, 吕丽霞, 脱登峰. 低温弱光胁迫下AMF和PGPR对紫罗兰生长及营养吸收的影响[J]. 浙江农业学报, 2025, 37(8): 1694-1705.

SHI Yangyang, LYU Lixia, TUO Dengfeng. Effects of AMF and PGPR on growth and nutrient absorption of Matthiola incana under low temperature and weak light stress[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1694-1705.

图/表 8

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胁迫处理 Stress treatment	接种处理 Inoculation treatment	菌根侵染率 Mycorrhizal colonization/%	丛枝着生率 Arbuscule rate/%	侵入点位数 Numbers of infection point/cm^-1	泡囊数 Vesicles/cm^-1
NN	CK	0	0	0	0
	AMF	49.3±0.6 bc	26.5±0.9 ab	7.3±1.2 ab	12.3±0.3 ab
	PGPR	0	0	0	0
	AMF+PGPR	61.5±0.9 a	29.2±1.6 a	9.0±0.6 a	14.7±0.3 a
LW	CK	0	0	0	0
	AMF	45.2±0.2 c	24.2±1.2 b	6.3±0.7 b	11.0±0.6 b
	PGPR	0	0	0	0
	AMF+PGPR	52.5±0.8 b	27.5±0.8 ab	7.6±0.3 ab	12.7±0.3 ab

胁迫处理 Stress treatment	接种处理 Inoculation treatment	菌根侵染率 Mycorrhizal colonization/%	丛枝着生率 Arbuscule rate/%	侵入点位数 Numbers of infection point/cm^-1	泡囊数 Vesicles/cm^-1
NN	CK	0	0	0	0
	AMF	49.3±0.6 bc	26.5±0.9 ab	7.3±1.2 ab	12.3±0.3 ab
	PGPR	0	0	0	0
	AMF+PGPR	61.5±0.9 a	29.2±1.6 a	9.0±0.6 a	14.7±0.3 a
LW	CK	0	0	0	0
	AMF	45.2±0.2 c	24.2±1.2 b	6.3±0.7 b	11.0±0.6 b
	PGPR	0	0	0	0
	AMF+PGPR	52.5±0.8 b	27.5±0.8 ab	7.6±0.3 ab	12.7±0.3 ab

胁迫处理 Stress treatment	接种处理 Inoculation treatment	株高 Plant height/cm	叶面积 Leaf area/cm²	地上部干重 Dry weight of shoots/g	根系干重 Dry weight of roots/g
NN	CK	15.2±0.4 d	671.0±20.1 de	2.15±0.08 cd	0.12±0.01 ef
	AMF	16.9±0.3 ab	855.3±28.9 c	2.29±0.17 bc	0.18±0.02 cd
	PGPR	17.1±0.2 ab	1 036.4±22.6 b	2.62±0.17 ab	0.22±0.01 c
	AMF+PGPR	17.4±0.1 a	1 305.8±47.4 a	2.74±0.12 a	0.48±0.01 a
LW	CK	11.2±0.3 e	589.1±7.4 e	1.87±0.08 d	0.09±0.01 f
	AMF	15.3±0.3 d	679.7±13.9 d	2.18±0.16 cd	0.15±0.01 de
	PGPR	16.1±0.2 cd	828.2±20.9 c	2.24±0.15 bcd	0.19±0.02 cd
	AMF+PGPR	16.5±0.2 bc	886.7±38.4 c	2.32±0.16 bc	0.28±0.02 b

胁迫处理 Stress treatment	接种处理 Inoculation treatment	株高 Plant height/cm	叶面积 Leaf area/cm²	地上部干重 Dry weight of shoots/g	根系干重 Dry weight of roots/g
NN	CK	15.2±0.4 d	671.0±20.1 de	2.15±0.08 cd	0.12±0.01 ef
	AMF	16.9±0.3 ab	855.3±28.9 c	2.29±0.17 bc	0.18±0.02 cd
	PGPR	17.1±0.2 ab	1 036.4±22.6 b	2.62±0.17 ab	0.22±0.01 c
	AMF+PGPR	17.4±0.1 a	1 305.8±47.4 a	2.74±0.12 a	0.48±0.01 a
LW	CK	11.2±0.3 e	589.1±7.4 e	1.87±0.08 d	0.09±0.01 f
	AMF	15.3±0.3 d	679.7±13.9 d	2.18±0.16 cd	0.15±0.01 de
	PGPR	16.1±0.2 cd	828.2±20.9 c	2.24±0.15 bcd	0.19±0.02 cd
	AMF+PGPR	16.5±0.2 bc	886.7±38.4 c	2.32±0.16 bc	0.28±0.02 b

胁迫处理 Stress treatment	接种处理 Inoculation treatment	氮含量 N content		磷含量 P content		钾含量 K content
胁迫处理 Stress treatment	接种处理 Inoculation treatment	地上部Shoot	根Root	地上部Shoot	根Root	地上部Shoot	根Root
NN	CK	28.1±2.2 de	11.6±1.0 f	55.2±1.2 e	27.7±1.1 f	11.5±0.3 e	3.0±0.2 f
	AMF	30.8±1.0 cde	27.0±0.9 cd	76.9±0.7 b	38.1±0.2 d	13.1±0.6 d	4.2±0.1 d
	PGPR	41.9±2.0 ab	30.9±1.3 b	94.9±1.8 a	40.6±0.6 c	21.6±0.5 a	5.2±0.1 c
	AMF+PGPR	45.9±4.1 a	35.5±0.9 a	97.6±2.1 a	55.3±1.1 a	21.4±0.7 a	8.4±0.2 a
LW	CK	26.3±3.0 e	11.4±0.5 f	41.5±1.4 f	20.7±0.5 g	8.7±0.5 f	2.3±0.1 g
	AMF	28.9±0.5 de	22.6±1.3 e	61.6±1.2 d	33.8±0.9 e	16.3±0.5 c	3.4±0.1 e
	PGPR	35.7±1.1 bcd	24.6±0.6 de	71.6±1.7 c	36.5±0.5 d	16.5±0.3 c	4.2±0.1 d
	AMF+PGPR	38.9±2.1 bc	29.6±0.9 bc	78.6±1.8 b	49.4±1.3 b	18.1±0.3 b	6.7±0.1 b

低温弱光胁迫下AMF和PGPR对紫罗兰生长及营养吸收的影响

Effects of AMF and PGPR on growth and nutrient absorption of Matthiola incana under low temperature and weak light stress

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