关键词: 大豆, 种质资源, SDS-PAGE, 7S球蛋白, 11S球蛋白

Abstract: Three hundred and twenty one soybean germplasm resources of Zhejiang Province from the germplasm bank of Hangzhou Sub-center of National Soybean Improvement were analyzed to investigate the 7S and 11S globulins contents using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed a great genetic diversity in the relative contents of 11S, 7S globulins and their subunits. The variation ranges of the relative content of 7S, 11S globulins and the 11S/7S ratio were 0.152-0.371, 0.428-0.794 and 1.269-4.008 respectively. There were seven germplasms, whose 11S/7S ratios were higher than 3.5, and protein content percent was higher than 45.0%. Four germplasms showed relative lower α',α-subunit content than others, and some unknown small peptides between 30 kD and 17 kD appeared. Significantly negative correlation was observed between the relative contents of 7S and 11S globulins. The 11S/7S ratios of different germplasms showed regional features according to their ecology regionalism. Germplasms from North Zhejiang showed the highest average 11S/7S ratio, and germplasms from South Zhejiang showed higher coefficient of variation in relative contents of 11S, 7S globulins than other areas.

Key words: soybean, germplasm, SDS-PAGE, 7S globulins, 11S globulins