SlTCP17基因生物信息学、亚细胞定位及表达分析

doi:10.3969/j.issn.1004-1524.20250715

浙江农业学报 ›› 2026, Vol. 38 ›› Issue (6): 1218-1227.DOI: 10.3969/j.issn.1004-1524.20250715

SlTCP17基因生物信息学、亚细胞定位及表达分析

娄世浩(), 徐金鑫, 嵇泽琳, 汪涛, 牛晓辉, 邓嘉英, 张涛, 国志信, 朴凤植, 董韩^*()

河南农业大学园艺学院, 河南郑州 450002

收稿日期:2025-11-21 出版日期:2026-06-25 发布日期:2026-07-14
作者简介:娄世浩,研究方向为设施蔬菜优质高效生产技术。E-mail:loushihao952714@163.com
通讯作者: ^*董韩,E-mail:440069@henau.edu.cn
基金资助:
国家自然科学基金(32573118);国家自然科学基金(32202579);河南省重大科技专项(241100110200);中国博士后科学基金面上项目(2022M711062)

Bioinformatic analysis, subcellular lcalization, and expression analysis of SlTCP17 gene

LOU Shihao(), XU Jinxin, JI Zelin, WANG Tao, NIU Xiaohui, DENG Jiaying, ZHANG Tao, GUO Zhixin, PIAO Fengzhi, DONG Han^*()

College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

Received:2025-11-21 Published:2026-06-25 Online:2026-07-14

摘要/Abstract

摘要：

TCP转录因子是调控植物生长发育与激素信号传导的核心元件。为了挖掘番茄(Solanum lycopersicum L.)中响应逆境胁迫的TCP家族成员,本研究克隆了1个TCP家族基因SlTCP17。通过生物信息学、亚细胞定位及实时荧光定量PCR(qRT-PCR)技术对其进行了系统性解析。结果显示,SlTCP17定位于细胞核,编码1个分子量为45.26 ku的不稳定亲水蛋白,其二级结构以无规卷曲(62.74%)为主。进化分析表明,SlTCP17 与SlTCP23 亲缘性最高,AtTCP8 次之。启动子区富含多种与胁迫、激素及光响应相关的顺式作用元件。该基因呈现叶片中高表达、根中低表达的组织特异性,其转录水平受干旱诱导,但被盐胁迫与辣椒疫霉菌侵染所抑制。这些结果表明,SlTCP17广泛参与了番茄的胁迫响应调控,为阐释其分子机制奠定了基础。

关键词: TCP 转录因子, 番茄, 逆境胁迫, 生物信息学分析, 亚细胞定位

Abstract:

TCP transcription factor is the core regulatory element to regulate plant growth and development and hormone signal transduction process. In order to discover the TCP family members in tomato (Solanum lycopersicum L.) who respond to adversity stress, a TCP family gene SlTCP17 was cloned in this study. We systematically analyzed it by bioinformatics, subcellular localization and qRT-PCR technology. The results showed that SlTCP17 was located in the nucleus, encoding an unstable hydrophilic protein with a relative molecular weight of 45.26 ku, and its secondary structure was mainly random coil (62.74%). Phylogenetic analysis showed that SlTCP17 and SlTCP23 have the highest affinity, followed by AtTCP8. The promoter region was rich in a variety of Cis-acting element related to stress, hormones and light response. The gene showed tissue specificity of high expression in leaves and low expression in roots, and its transcription level was induced by drought, but inhibited by salt and Phytophthora capsici infection. These findings indicate that SlTCP17 is widely involved in the regulation of tomato stress response, which lays a foundation for explaining its molecular mechanism.

Key words: TCP transcription factor, tomato, adversity stress, bioinformatics analysis, subcellular localization

中图分类号:

S685.12

娄世浩, 徐金鑫, 嵇泽琳, 汪涛, 牛晓辉, 邓嘉英, 张涛, 国志信, 朴凤植, 董韩. SlTCP17基因生物信息学、亚细胞定位及表达分析[J]. 浙江农业学报, 2026, 38(6): 1218-1227.

LOU Shihao, XU Jinxin, JI Zelin, WANG Tao, NIU Xiaohui, DENG Jiaying, ZHANG Tao, GUO Zhixin, PIAO Fengzhi, DONG Han. Bioinformatic analysis, subcellular lcalization, and expression analysis of SlTCP17 gene[J]. Acta Agriculturae Zhejiangensis, 2026, 38(6): 1218-1227.

图/表 11

表1 引物序列

Table 1 Primer sequence

引物名称 Primer name	引物序列(5'→3') Primer sequence(5'→3')	注释 Annotation
RT-SlTCP17-F	AGTTATAGCTGCTACGGG	qRT-PCR
RT-SlTCP17-R	TTGAGATGGTACGGACAT
RT-ACTIN2-F	TGTCCCTATTTACGAGGGTTATGC
RT-ACTIN2-R	CAGTTAAATCACGACCAGCAAGAT
RT-UBI3-F	GCCGACTACAACATCCAGAAGG
RT-UBI3-R	TGCAACACAGCGAGCTTAACC
GFP-SlTCP17-F	ctctcgagctttcgc gagctcATGGAAGGAGGAGGAGGTGACG	融合绿色荧光蛋白Green fluorescent protein
GFP-SlTCP17-R	gcccttgctcaccat ggatccGGAATTATGATGGCTTGTTGAATCA

图1 SlTCP17蛋白保守结构域分析

Fig.1 Conservative domain analysis of SlTCP17 protein

图2 SlTCP17蛋白的亲水性预测

Fig.2 Hydrophilicity prediction of SlTCP17 protein

图3 SlTCP17蛋白跨膜区域(A)及信号肽(B)预测 Transmembrane,跨膜区氨基酸;Inside,膜内氨基酸;Outside,膜外氨基酸。S-score,信号肽分数;C-score,剪切位点分数;Y-score,综合剪切位点分数。

Fig.3 Prediction of transmembrane region (A) and signal peptide (B) of SlTCP17 protein Transmembrane, Amino acids in the transmembrane region; Inside, Amino acids on the intramembrane side; Outside, Amino acids on the extramembrane side. S-score, Signal peptide score; C-score, Cleavage site score; Y-score, Comprehensive cleavage site score.

图4 SlTCP17蛋白结构预测 Helix,α螺旋;Sheet,β折叠;Turn,β转角;Coil,无规卷曲。

Fig.4 Prediction of protein structure of SlTCP17 Helix, α Helix; Sheet, β Sheet ; Turn, β Turn; Coil, Random coil.

图5 番茄、拟南芥TCP蛋白的系统发育树

Fig.5 Phylogenetic tree of TCP protein in tomato and Arabidopsis thaliana

表2 SlTCP17基因启动子顺式作用元件分析

Table 2 Cis-acting element analysis of SlTCP17 gene promoter

名称 Name	序列 Sequence	功能 Function	数量 Number
TATA-box	ATATAA	核心启动子元件在转录开始的-30左右 Core promoter element around -30 of transcription start	4
	ATATAT		7
	ATTATA		4
	TACATAAA		1
	TATA		21
	TATAA		4
	TATACA		2
	TATATA		11
	TATATAA		2
	TATTTAAA		1
ABRE	TACGGTC	参与脱落酸反应的顺式作用元件 Cis-acting element involved in the abscisic acid responsiveness	1
	CACGTG		1
	ACGTG		1
ARE	AAACCA	对厌氧诱导必不可少的顺式作用元件 Cis-acting element essential for the anaerobic induction	1
Box 4	ATTAAT	与光反应有关的保守DNA模块的一部分 Part of a conserved DNA module involved in light responsiveness	4
CAAT-box	CAAAT	启动子和增强子区域中常见的顺式作用元件 Common cis-acting element in promoter and enhancer regions	11
	CCAAT		3
CCAAT-box	CAACGG	MYBHv1结合位点MYBHv1 binding site	1
CGTCA-motif	CGTCA	参与MeJA反应的顺式作用元件Cis-acting element involved in the MeJA-responsiveness	3
TGACG-motif	TGACG	光响应元件的一部分Part of a light responsive element	3
chs-CMA1a	TTACTTAA		1
GA-motif	ATAGATAA		1
GATA-motif	GATAGGA		1
TCT-motif	TCTTAC		1
GT1-motif	CACGTG		2
	GGTTAA		1
G-Box	CACGAC	参与光响应的顺式作用元件Cis-acting element involved in light responsiveness	1
	CACGTG		2
HD-Zip 3	GTAAT(G/C)ATTAC	蛋白质结合位点Protein binding site	1
MRE	AACCTAA	MYB结合位点参与光响应MYB binding site involved in light responsiveness	2
P-box	CCTTTTG	赤霉素响应元件Gibberellin-responsive element	2
TGA-element	AACGAC	生长素响应元件Auxin-responsive element	2

图6 SlTCP17基因的克隆

Fig.6 Cloning of SlTCP17 gene

图7 SlTCP17蛋白的亚细胞定位 GFP,绿色荧光蛋白信号;Nucleus,细胞核定位;Bright,明场视野;Merged,通道叠加。

Fig.7 Subcellular localization of SlTCP17 protein GFP, Green fluorescent protein signal; Nucleus, Nuclear localization; Bright, Bright field of vision; Merged, Channel superposition.

图8 SlTCP17在番茄不同组织中的表达情况柱上无相同小写字母表示不同组间差异显著(p<0.05),图9同。

Fig.8 Expression of SlTCP17 in different tissues of tomato The absence of identical lowercase letters on the column indicates significant differences (p<0.05) between groups, as shown in Fig.9.

图9 SlTCP17在盐胁迫(A)、辣椒疫霉菌侵染(B)、低温(C)及干旱(D)胁迫条件下的表达情况

Fig.9 The expression of SlTCP17 under salt stress(A), Phytophthora capsici infection (B), low temperature (C) and drought stress (D)

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SlTCP17基因生物信息学、亚细胞定位及表达分析

Bioinformatic analysis, subcellular lcalization, and expression analysis of SlTCP17 gene

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