Genetic diversity analysis of Brassica oleracea L. var. italica with SSR markers

doi:10.3969/j.issn.1004-1524.2017.02.08

›› 2017, Vol. 29 ›› Issue (2): 228-235.DOI: 10.3969/j.issn.1004-1524.2017.02.08

• Horticultural Science • Previous Articles Next Articles

Genetic diversity analysis of Brassica oleracea L. var. italica with SSR markers

ZHANG Zhixian, HE Daogen^*, ZHU Changzhi, TAN Guoyin, GAO Xu

Taizhou Academy of Agricultural Sciences, Linhai 317000, China

Received:2016-10-18 Online:2017-02-15 Published:2017-03-06

Abstract

Abstract: Simple sequence repeat (SSR) markers were used to analyze genetic diversity among 28 broccoli (Brassica oleracea L. var. italica) materials. Fourteen pairs of SSR marker primers selected from 50 pairs of SSR primers generated 94 polymorphic bands among these 28 materials. The polymorphism information content (PIC) ranged from 0.20 to 0.91. The overall genetic similarity values ranged from 0.585 1 to 0.904 3 with an average value of 0.728 9. Among them, TN12 and TN14 were the most similar while TN503 and TN597 were the most distant ones with genetic similarity values of 0.904 3 and 0.585 1, respectively. The cluster analysis divided the 28 broccoli materials into two clades (Ⅰand Ⅱ). Clade Ⅰ was constituted of 26 broccoli inbred lines and clade Ⅱ were formed by TN539 and TN597. While the result of principal coordinates analysis showed that all 28 materials were grouped into 4 clades, which consist of 2, 6, 6, 14 broccoli germplasm materials, respectively. Both of the two methods showed that TN539 and TN597 had a low genetic similarity with other 26 germplasm materials. These results revealed that 28 broccoli materials had a narrow genetic base while part of them had wide variations, which provide information for their use and the hybrid parents choice.

Key words: broccoli, SSR, cluster analysis, principal coordinates analysis, genetic diversity

CLC Number:

S635.3

ZHANG Zhixian, HE Daogen, ZHU Changzhi, TAN Guoyin, GAO Xu. Genetic diversity analysis of Brassica oleracea L. var. italica with SSR markers[J]. , 2017, 29(2): 228-235.

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Genetic diversity analysis of Brassica oleracea L. var. italica with SSR markers

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