Isolation, identification and characteristics of an extreme salt-tolerant strain

doi:10.3969/j.issn.1004-1524.2018.10.16

›› 2018, Vol. 30 ›› Issue (10): 1722-1728.DOI: 10.3969/j.issn.1004-1524.2018.10.16

• Plant Protection • Previous Articles Next Articles

Isolation, identification and characteristics of an extreme salt-tolerant strain

FAN Ting, WU Lingli, LI Yunyun, LIU Yalou, LIU Ru, YE Wenling, CHEN Haiyan, PAN Dandan

Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

Received:2018-01-30 Online:2018-10-25 Published:2018-11-02

Abstract

Abstract: An extremely salt-tolerant strain named NY-1 was isolated from the fresh filtrate of Wushan hazardous waste landfill. Based on morphological, gram staining and 16S rDNA sequence analysis, the strain was identified as Achromobacter sp.. The strain NY-1 can survive under salinity 0-30%. Effects of different medium, salinity gradient, pH and temperature on the growth of strain NY-1 were studied, the results showed that the optimum conditions were salinity 10%-20%, pH 7.0, 30 ℃ in the LB medium. The salt-tolerant mechanism of strain NY-1 was analyzed by the changing of intracellular cation in different salinity. Under low salinity, the strain NY-1 absorbed K⁺ and Na⁺ to maintain the osmotic balance inside the cell, and released Ca²⁺ and Mg²⁺ to maintain neutral environment inside the cell. When the salinity was above 20%, the strain NY-1 resisted the interference of high salinity by sustaining to release Na⁺ to the extra-cellular environment. In conclusion, the strain NY-1 shows the potential of application in biological treatment of high salinity wastewater and soil bioremediation.

Key words: salt-tolerant strains, 16S rDNA, identification, characteristics of growth, salt tolerance mechanism

CLC Number:

Q939.99
X172

FAN Ting, WU Lingli, LI Yunyun, LIU Yalou, LIU Ru, YE Wenling, CHEN Haiyan, PAN Dandan. Isolation, identification and characteristics of an extreme salt-tolerant strain[J]. , 2018, 30(10): 1722-1728.

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Isolation, identification and characteristics of an extreme salt-tolerant strain

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