A rapid and precise SSR-based procedure for seed quality surveying of Vigna unguiculata ssp. sesquipedalis (L.) Verd．

›› 2010, Vol. 22 ›› Issue (6): 727-730.

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A rapid and precise SSR-based procedure for seed quality surveying of Vigna unguiculata ssp. sesquipedalis (L.) Verd．

LU Zhong-fu;XU Pei;WANG Bao-geng;LIU Yong-hua;WU Xiao-hua;Hu Ting-ting;LI Guo-jing*

Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-11-25 Published:2010-11-25

Abstract

Abstract: Current application of large number of commercial asparagus bean cultivars with very high genetic similarity caused more and more disputes on seed quality. In this research, a set of SSR markers for asparagus bean were developed based on a bioinformatics-based approach. Ten of these markers were defined as diagnostic markers by genotyping a core collection of asparagus bean consisting of 44 lines. Based on this, a rapid and precise SSR-based procedure for asparagus bean seed quality surveying was established, which would meet the demand of rapidly developing asparagus bean industry．

Key words: asparagus bean, SSR, purity surveying, genetic fingerprint

LU Zhong-fu;XU Pei;WANG Bao-geng;LIU Yong-hua;WU Xiao-hua;Hu Ting-ting;LI Guo-jing*. A rapid and precise SSR-based procedure for seed quality surveying of Vigna unguiculata ssp. sesquipedalis (L.) Verd．[J]. , 2010, 22(6): 727-730.

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A rapid and precise SSR-based procedure for seed quality surveying of Vigna unguiculata ssp. sesquipedalis (L.) Verd．

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