Abstract:  In the present study， a rapid and simple method to detect soybean mixture in tea by loop\|mediated isothermal amplification （LAMP） was established. On the basis of the endogenous gene lectin of soybean， six primers were designed and characteristic waterfall\|like bands by LAMP were acquired. Specificity tests showed that the primers were specific for soybean detection. Sensitivity test showed that LAMP was 10 times higher sensitive than fluorescence PCR. According to the optimized temperature， concentration of betaine， Mg2+， and dNTP， the entire testing cycle was about 1 h. Besides， it was convenient to observe the results by using SYBR green I. Thus， the established LAMP method could be useful for soybean component detection from tea samples in the near future.

Key words: tea, soybean, loop\, mediated isothermal amplification （LAMP）, detection