Abstract: In order to find out the differences among Grifola frondosa with different cultivation methods，monitoring was conducted for 3 years in 10 growers. Both heavy metals and pesticide residues in spring Grifola frondosa， autumn Grifola frondosa with overlying soil， autumn Grifola frondosa without overlying soil， and their corresponding culture media were determined. The results showed that Pb content in autumn Grifola frondosa with overlying soil was 2.81 mg·kg-1， exceeding the maximum limit （1.0 mg·kg-1）set in GB 2762—2012， which may be caused by the high concentration of Pb in overlying soil. The cultivation method of autumn Grifola frondosa without overlying soil could control the enrichment of Pb. The result also showed that Pb concentration in autumn Grifola frondosa without overlying soil was 0.120 mg·kg-1，which was within the maximum limit. The organophosphorus， organochlorine， pyrethroid and carbamate pesticide residues in spring Grifola frondosa，autumn Grifola frondosa with overlying soil， autumn Grifola frondosa without overlying soil were not detected. Se contents in autumn Grifola frondosa with overlying soil， spring Grifola frondosa，autumn Grifola frondosa without overlying soil were 0.200，0.080， 0.133 mg·kg-1， respectively. The cultivation method of autumn Grifola frondosa without overlying soil could block the enrichment of Pb through the culture medium. The Pb， Cd， carbendazim， carbofuran， deltamethrin， bifenthrin， fenpropathrin， beta cypermethrin in Grifola frondosa without overlying soil was within the national standard. Grifola frondosa cultivated without overlying soil had the same yield as with overlying soil， but the income was doubled. In conclusion， concentrations of heavy metals， pesticide residues and Se in Grifola frondosa with different culture medium were determined in the present stuty，and the results may provide technical support for quality and safety control of Grifola frondosa cultivation.

Key words: Grifola frondosa, pesticide residue, harmful heavy metals, Se-rich